Preparation and purification of mono-ubiquitinated proteins using Avi-tagged ubiquitin

Site-specific conjugation of ubiquitin onto a range of DNA repair proteins regulates their critical functions in the DNA damage response. Biochemical and structural characterization of these functions are limited by an absence of tools for the purification of DNA repair proteins in purely the ubiqui...

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Detalles Bibliográficos
Autores principales: Tan, Winnie, Murphy, Vincent J., Charron, Aude, van Twest, Sylvie, Sharp, Michael, Constantinou, Angelos, Parker, Michael W., Crismani, Wayne, Bythell-Douglas, Rohan, Deans, Andrew J.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Public Library of Science 2020
Materias:
Research Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7039436/
https://www.ncbi.nlm.nih.gov/pubmed/32092106
http://dx.doi.org/10.1371/journal.pone.0229000

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