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Methodology and validation of a new tandem mass spectrometer method for the quantification of inorganic and organic (18)O-phosphate species
Phosphorus (P) fertilizers are crucial to achieve peak productivity in agricultural systems. However, the fate of P fertilizers via microorganism incorporation and the exchange processes between soil pools is not well understood. (18)Oxygen-labelled phosphate ((18)O- P(i)) can be tracked as it cycle...
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Public Library of Science
2020
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7039501/ https://www.ncbi.nlm.nih.gov/pubmed/32092104 http://dx.doi.org/10.1371/journal.pone.0229172 |
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author | Schryer, Aimée Bradshaw, Kris Siciliano, Steven D. |
author_facet | Schryer, Aimée Bradshaw, Kris Siciliano, Steven D. |
author_sort | Schryer, Aimée |
collection | PubMed |
description | Phosphorus (P) fertilizers are crucial to achieve peak productivity in agricultural systems. However, the fate of P fertilizers via microorganism incorporation and the exchange processes between soil pools is not well understood. (18)Oxygen-labelled phosphate ((18)O- P(i)) can be tracked as it cycles through soil systems. Our study describes biological and geochemical P dynamics using a tandem mass spectrometry (MS/MS) method for the absolute quantification of (18)O- P(i). Soil microcosms underwent three treatments: (i) (18)O- P(i), (ii) unlabelled phosphate ((16)O- P(i)) or (iii) Milli-Q control, dissolved in a bio-stimulatory solution. During a 6-week series the microcosms were sampled to measure P by Hedley sequential fractionation and DNA extraction samples digested to 3′-deoxynucleoside 5′-monophosphates (dNMP). A MS/MS attached to a HPLC analyzed each P-species through collision-induced dissociation. The resin-extractable and bicarbonate (18)O- P(i) and (16)O- P(i) fractions displayed similar precipitation and adsorption-desorption trends. Biotic activity measured in the NaOH and dNMP fractions rapidly delabelled (18)O- P(i); however, the MS/MS measured some (18)O that remained between the P backbone and deoxyribose sugars. After 6 weeks, the (18)O- P(i) had not reached the HCl soil pool, highlighting the long-term nature of P movement. Our methodology improves on previous isotopic tracking methods as endogenous P does not dilute the system, unlike (32)P techniques, and measured total P is not a ratio, dissimilar from natural abundance techniques. Measuring (18)O- P(i) using MS/MS provides information to enhance land sustainability and stewardship practices regardless of soil type by understanding both the inorganic movement of P fertilizers and the dynamic P pool in microbial DNA. |
format | Online Article Text |
id | pubmed-7039501 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2020 |
publisher | Public Library of Science |
record_format | MEDLINE/PubMed |
spelling | pubmed-70395012020-03-06 Methodology and validation of a new tandem mass spectrometer method for the quantification of inorganic and organic (18)O-phosphate species Schryer, Aimée Bradshaw, Kris Siciliano, Steven D. PLoS One Research Article Phosphorus (P) fertilizers are crucial to achieve peak productivity in agricultural systems. However, the fate of P fertilizers via microorganism incorporation and the exchange processes between soil pools is not well understood. (18)Oxygen-labelled phosphate ((18)O- P(i)) can be tracked as it cycles through soil systems. Our study describes biological and geochemical P dynamics using a tandem mass spectrometry (MS/MS) method for the absolute quantification of (18)O- P(i). Soil microcosms underwent three treatments: (i) (18)O- P(i), (ii) unlabelled phosphate ((16)O- P(i)) or (iii) Milli-Q control, dissolved in a bio-stimulatory solution. During a 6-week series the microcosms were sampled to measure P by Hedley sequential fractionation and DNA extraction samples digested to 3′-deoxynucleoside 5′-monophosphates (dNMP). A MS/MS attached to a HPLC analyzed each P-species through collision-induced dissociation. The resin-extractable and bicarbonate (18)O- P(i) and (16)O- P(i) fractions displayed similar precipitation and adsorption-desorption trends. Biotic activity measured in the NaOH and dNMP fractions rapidly delabelled (18)O- P(i); however, the MS/MS measured some (18)O that remained between the P backbone and deoxyribose sugars. After 6 weeks, the (18)O- P(i) had not reached the HCl soil pool, highlighting the long-term nature of P movement. Our methodology improves on previous isotopic tracking methods as endogenous P does not dilute the system, unlike (32)P techniques, and measured total P is not a ratio, dissimilar from natural abundance techniques. Measuring (18)O- P(i) using MS/MS provides information to enhance land sustainability and stewardship practices regardless of soil type by understanding both the inorganic movement of P fertilizers and the dynamic P pool in microbial DNA. Public Library of Science 2020-02-24 /pmc/articles/PMC7039501/ /pubmed/32092104 http://dx.doi.org/10.1371/journal.pone.0229172 Text en © 2020 Schryer et al http://creativecommons.org/licenses/by/4.0/ This is an open access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/) , which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited. |
spellingShingle | Research Article Schryer, Aimée Bradshaw, Kris Siciliano, Steven D. Methodology and validation of a new tandem mass spectrometer method for the quantification of inorganic and organic (18)O-phosphate species |
title | Methodology and validation of a new tandem mass spectrometer method for the quantification of inorganic and organic (18)O-phosphate species |
title_full | Methodology and validation of a new tandem mass spectrometer method for the quantification of inorganic and organic (18)O-phosphate species |
title_fullStr | Methodology and validation of a new tandem mass spectrometer method for the quantification of inorganic and organic (18)O-phosphate species |
title_full_unstemmed | Methodology and validation of a new tandem mass spectrometer method for the quantification of inorganic and organic (18)O-phosphate species |
title_short | Methodology and validation of a new tandem mass spectrometer method for the quantification of inorganic and organic (18)O-phosphate species |
title_sort | methodology and validation of a new tandem mass spectrometer method for the quantification of inorganic and organic (18)o-phosphate species |
topic | Research Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7039501/ https://www.ncbi.nlm.nih.gov/pubmed/32092104 http://dx.doi.org/10.1371/journal.pone.0229172 |
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