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Dorsal Hippocampus ERK2 Signaling Mediates Anxiolytic-Related Behavior in Male Rats

BACKGROUND: Anxiety disorders are the most common neuropathologies worldwide, but the precise neuronal mechanisms that underlie these disorders remain unknown. The hippocampus plays a role in mediating anxiety-related responses, which can be modeled in rodents using behavioral assays, such as the el...

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Autores principales: Sierra-Fonseca, Jorge A., Parise, Lyonna F., Flores-Ramirez, Francisco J., Robles, Eden H., Garcia-Carachure, Israel, Iñiguez, Sergio D.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: SAGE Publications 2019
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7039622/
https://www.ncbi.nlm.nih.gov/pubmed/32095734
http://dx.doi.org/10.1177/2470547019897030
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author Sierra-Fonseca, Jorge A.
Parise, Lyonna F.
Flores-Ramirez, Francisco J.
Robles, Eden H.
Garcia-Carachure, Israel
Iñiguez, Sergio D.
author_facet Sierra-Fonseca, Jorge A.
Parise, Lyonna F.
Flores-Ramirez, Francisco J.
Robles, Eden H.
Garcia-Carachure, Israel
Iñiguez, Sergio D.
author_sort Sierra-Fonseca, Jorge A.
collection PubMed
description BACKGROUND: Anxiety disorders are the most common neuropathologies worldwide, but the precise neuronal mechanisms that underlie these disorders remain unknown. The hippocampus plays a role in mediating anxiety-related responses, which can be modeled in rodents using behavioral assays, such as the elevated plus maze. Yet, the molecular markers that underlie affect-related behavior on the elevated plus maze are not well understood. METHODS: We used herpes simplex virus vector delivery to overexpress extracellular signal-regulated kinase-2, a signaling molecule known to be involved in depression and anxiety, within the dorsal hippocampus of adult Sprague-Dawley male rats. Three days post virus delivery, we assessed anxiety-like responses on the elevated plus maze or general locomotor activity on the open field test. RESULTS: When compared to controls, rats overexpressing extracellular signal-regulated kinase-2 in the dorsal hippocampus displayed an anxiolytic-like phenotype, per increases in time spent in the open arms, and less time in the closed arms, of the elevated plus maze. Furthermore, no changes in locomotor activity as a function of virus infusion were observed on the open field test between the experimental groups. CONCLUSION: This investigation demonstrates that virus-mediated increases of extracellular signal-regulated kinase-2 signaling, within the hippocampus, plays a critical role in decreasing anxiogenic responses on the rat elevated plus maze. As such, our data provide construct validity, at least in part, to the molecular mechanisms that mediate anxiolytic-like behavior in rodent models for the study of anxiety.
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spelling pubmed-70396222020-02-24 Dorsal Hippocampus ERK2 Signaling Mediates Anxiolytic-Related Behavior in Male Rats Sierra-Fonseca, Jorge A. Parise, Lyonna F. Flores-Ramirez, Francisco J. Robles, Eden H. Garcia-Carachure, Israel Iñiguez, Sergio D. Chronic Stress (Thousand Oaks) Original Article BACKGROUND: Anxiety disorders are the most common neuropathologies worldwide, but the precise neuronal mechanisms that underlie these disorders remain unknown. The hippocampus plays a role in mediating anxiety-related responses, which can be modeled in rodents using behavioral assays, such as the elevated plus maze. Yet, the molecular markers that underlie affect-related behavior on the elevated plus maze are not well understood. METHODS: We used herpes simplex virus vector delivery to overexpress extracellular signal-regulated kinase-2, a signaling molecule known to be involved in depression and anxiety, within the dorsal hippocampus of adult Sprague-Dawley male rats. Three days post virus delivery, we assessed anxiety-like responses on the elevated plus maze or general locomotor activity on the open field test. RESULTS: When compared to controls, rats overexpressing extracellular signal-regulated kinase-2 in the dorsal hippocampus displayed an anxiolytic-like phenotype, per increases in time spent in the open arms, and less time in the closed arms, of the elevated plus maze. Furthermore, no changes in locomotor activity as a function of virus infusion were observed on the open field test between the experimental groups. CONCLUSION: This investigation demonstrates that virus-mediated increases of extracellular signal-regulated kinase-2 signaling, within the hippocampus, plays a critical role in decreasing anxiogenic responses on the rat elevated plus maze. As such, our data provide construct validity, at least in part, to the molecular mechanisms that mediate anxiolytic-like behavior in rodent models for the study of anxiety. SAGE Publications 2019-12-24 /pmc/articles/PMC7039622/ /pubmed/32095734 http://dx.doi.org/10.1177/2470547019897030 Text en © The Author(s) 2019 https://creativecommons.org/licenses/by-nc/4.0/ Creative Commons Non Commercial CC BY-NC: This article is distributed under the terms of the Creative Commons Attribution-NonCommercial 4.0 License (https://creativecommons.org/licenses/by-nc/4.0/) which permits non-commercial use, reproduction and distribution of the work without further permission provided the original work is attributed as specified on the SAGE and Open Access pages (https://us.sagepub.com/en-us/nam/open-access-at-sage).
spellingShingle Original Article
Sierra-Fonseca, Jorge A.
Parise, Lyonna F.
Flores-Ramirez, Francisco J.
Robles, Eden H.
Garcia-Carachure, Israel
Iñiguez, Sergio D.
Dorsal Hippocampus ERK2 Signaling Mediates Anxiolytic-Related Behavior in Male Rats
title Dorsal Hippocampus ERK2 Signaling Mediates Anxiolytic-Related Behavior in Male Rats
title_full Dorsal Hippocampus ERK2 Signaling Mediates Anxiolytic-Related Behavior in Male Rats
title_fullStr Dorsal Hippocampus ERK2 Signaling Mediates Anxiolytic-Related Behavior in Male Rats
title_full_unstemmed Dorsal Hippocampus ERK2 Signaling Mediates Anxiolytic-Related Behavior in Male Rats
title_short Dorsal Hippocampus ERK2 Signaling Mediates Anxiolytic-Related Behavior in Male Rats
title_sort dorsal hippocampus erk2 signaling mediates anxiolytic-related behavior in male rats
topic Original Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7039622/
https://www.ncbi.nlm.nih.gov/pubmed/32095734
http://dx.doi.org/10.1177/2470547019897030
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