Cargando…
Disclosing the Potential of the SARP-Type Regulator PapR2 for the Activation of Antibiotic Gene Clusters in Streptomycetes
Streptomyces antibiotic regulatory protein (SARP) family regulators are well-known activators of antibiotic biosynthesis in streptomycetes. The respective genes occur in various types of antibiotic gene clusters encoding, e.g., for polyketides, ribosomally and non-ribosomally synthesized peptides, o...
Autores principales: | , , , , |
---|---|
Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Frontiers Media S.A.
2020
|
Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7040171/ https://www.ncbi.nlm.nih.gov/pubmed/32132989 http://dx.doi.org/10.3389/fmicb.2020.00225 |
_version_ | 1783500927561367552 |
---|---|
author | Krause, Janina Handayani, Ira Blin, Kai Kulik, Andreas Mast, Yvonne |
author_facet | Krause, Janina Handayani, Ira Blin, Kai Kulik, Andreas Mast, Yvonne |
author_sort | Krause, Janina |
collection | PubMed |
description | Streptomyces antibiotic regulatory protein (SARP) family regulators are well-known activators of antibiotic biosynthesis in streptomycetes. The respective genes occur in various types of antibiotic gene clusters encoding, e.g., for polyketides, ribosomally and non-ribosomally synthesized peptides, or β-lactam antibiotics. We found that overexpression of the SARP-type regulator gene papR2 from Streptomyces pristinaespiralis in Streptomyces lividans leads to the activation of the silent undecylprodigiosin (Red) gene cluster. The activation happens upon the inducing function of PapR2, which takes over the regulatory role of RedD, the latter of which is the intrinsic SARP regulator of Red biosynthesis in S. lividans. Due to the broad abundance of SARP genes in different antibiotic gene clusters of various actinomycetes and the uniform activating principle of the encoded regulators, we suggest that this type of regulator is especially well suited to be used as an initiator of antibiotic biosynthesis in actinomycetes. Here, we report on a SARP-guided strategy to activate antibiotic gene clusters. As a proof of principle, we present the PapR2-driven activation of the amicetin/plicacetin gene cluster in the novel Indonesian strain isolate Streptomyces sp. SHP22-7. |
format | Online Article Text |
id | pubmed-7040171 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2020 |
publisher | Frontiers Media S.A. |
record_format | MEDLINE/PubMed |
spelling | pubmed-70401712020-03-04 Disclosing the Potential of the SARP-Type Regulator PapR2 for the Activation of Antibiotic Gene Clusters in Streptomycetes Krause, Janina Handayani, Ira Blin, Kai Kulik, Andreas Mast, Yvonne Front Microbiol Microbiology Streptomyces antibiotic regulatory protein (SARP) family regulators are well-known activators of antibiotic biosynthesis in streptomycetes. The respective genes occur in various types of antibiotic gene clusters encoding, e.g., for polyketides, ribosomally and non-ribosomally synthesized peptides, or β-lactam antibiotics. We found that overexpression of the SARP-type regulator gene papR2 from Streptomyces pristinaespiralis in Streptomyces lividans leads to the activation of the silent undecylprodigiosin (Red) gene cluster. The activation happens upon the inducing function of PapR2, which takes over the regulatory role of RedD, the latter of which is the intrinsic SARP regulator of Red biosynthesis in S. lividans. Due to the broad abundance of SARP genes in different antibiotic gene clusters of various actinomycetes and the uniform activating principle of the encoded regulators, we suggest that this type of regulator is especially well suited to be used as an initiator of antibiotic biosynthesis in actinomycetes. Here, we report on a SARP-guided strategy to activate antibiotic gene clusters. As a proof of principle, we present the PapR2-driven activation of the amicetin/plicacetin gene cluster in the novel Indonesian strain isolate Streptomyces sp. SHP22-7. Frontiers Media S.A. 2020-02-18 /pmc/articles/PMC7040171/ /pubmed/32132989 http://dx.doi.org/10.3389/fmicb.2020.00225 Text en Copyright © 2020 Krause, Handayani, Blin, Kulik and Mast. http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms. |
spellingShingle | Microbiology Krause, Janina Handayani, Ira Blin, Kai Kulik, Andreas Mast, Yvonne Disclosing the Potential of the SARP-Type Regulator PapR2 for the Activation of Antibiotic Gene Clusters in Streptomycetes |
title | Disclosing the Potential of the SARP-Type Regulator PapR2 for the Activation of Antibiotic Gene Clusters in Streptomycetes |
title_full | Disclosing the Potential of the SARP-Type Regulator PapR2 for the Activation of Antibiotic Gene Clusters in Streptomycetes |
title_fullStr | Disclosing the Potential of the SARP-Type Regulator PapR2 for the Activation of Antibiotic Gene Clusters in Streptomycetes |
title_full_unstemmed | Disclosing the Potential of the SARP-Type Regulator PapR2 for the Activation of Antibiotic Gene Clusters in Streptomycetes |
title_short | Disclosing the Potential of the SARP-Type Regulator PapR2 for the Activation of Antibiotic Gene Clusters in Streptomycetes |
title_sort | disclosing the potential of the sarp-type regulator papr2 for the activation of antibiotic gene clusters in streptomycetes |
topic | Microbiology |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7040171/ https://www.ncbi.nlm.nih.gov/pubmed/32132989 http://dx.doi.org/10.3389/fmicb.2020.00225 |
work_keys_str_mv | AT krausejanina disclosingthepotentialofthesarptyperegulatorpapr2fortheactivationofantibioticgeneclustersinstreptomycetes AT handayaniira disclosingthepotentialofthesarptyperegulatorpapr2fortheactivationofantibioticgeneclustersinstreptomycetes AT blinkai disclosingthepotentialofthesarptyperegulatorpapr2fortheactivationofantibioticgeneclustersinstreptomycetes AT kulikandreas disclosingthepotentialofthesarptyperegulatorpapr2fortheactivationofantibioticgeneclustersinstreptomycetes AT mastyvonne disclosingthepotentialofthesarptyperegulatorpapr2fortheactivationofantibioticgeneclustersinstreptomycetes |