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Effect of autophagy induction and cathepsin B inhibition on developmental competence of poor quality bovine oocytes

The present study investigated the effect of autophagy induction and cathepsin B (CTSB) inhibition on developmental competence of poor quality oocytes. Bovine cumulus oocyte complexes (COCs) were classified as good or poor according to their morphology. Autophagy activity was detected in good and po...

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Autores principales: LI, Jianye, BALBOULA, Ahmed Zaky, ABOELENAIN, Mansour, FUJII, Takashi, MORIYASU, Satoru, BAI, Hanako, KAWAHARA, Manabu, TAKAHASHI, Masashi
Formato: Online Artículo Texto
Lenguaje:English
Publicado: The Society for Reproduction and Development 2019
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7040212/
https://www.ncbi.nlm.nih.gov/pubmed/31875588
http://dx.doi.org/10.1262/jrd.2019-123
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author LI, Jianye
BALBOULA, Ahmed Zaky
ABOELENAIN, Mansour
FUJII, Takashi
MORIYASU, Satoru
BAI, Hanako
KAWAHARA, Manabu
TAKAHASHI, Masashi
author_facet LI, Jianye
BALBOULA, Ahmed Zaky
ABOELENAIN, Mansour
FUJII, Takashi
MORIYASU, Satoru
BAI, Hanako
KAWAHARA, Manabu
TAKAHASHI, Masashi
author_sort LI, Jianye
collection PubMed
description The present study investigated the effect of autophagy induction and cathepsin B (CTSB) inhibition on developmental competence of poor quality oocytes. Bovine cumulus oocyte complexes (COCs) were classified as good or poor according to their morphology. Autophagy activity was detected in good and poor germinal vesicle (GV) oocytes. Then E-64, a CTSB inhibitor, rapamycin (Rapa), an autophagy inducer, and combined administration was achieved during in vitro maturation (IVM) of poor quality COCs followed by detection of autophagy activity. In the next experiment, E-64, Rapa, and E64 + Rapa, were added during IVM to good and poor quality COCs followed by in vitro fertilization and culture for 8 days to investigate whether inhibition of CTSB and/or induction of autophagy improve embryonic development and quality. Autophagy activity was significantly lower in poor quality GV oocytes than in good quality ones. E-64, Rapa and E-64 + Rapa treatment during IVM significantly increased autophagy activity in poor quality oocytes. Addition of Rapa in good quality COCs did not increase the blastocyst rate, whereas E-64 increased the blastocyst rate and total cell number (TCN) with decreasing TUNEL-positive cells. In contrast, Rapa treatment in poor quality COCs significantly increased the blastocyst rate and TCN with decreasing TUNEL-positive cells. These results indicate oocyte quality has different responses to intracellular autophagy induction and CTSB activity control by potential autophagy and catabolic status, however, synergetic effect of autophagy induction and CTSB inhibition can increase developmental competence of both good and poor quality COCs, especially rescue effect in poor quality COCs.
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spelling pubmed-70402122020-03-02 Effect of autophagy induction and cathepsin B inhibition on developmental competence of poor quality bovine oocytes LI, Jianye BALBOULA, Ahmed Zaky ABOELENAIN, Mansour FUJII, Takashi MORIYASU, Satoru BAI, Hanako KAWAHARA, Manabu TAKAHASHI, Masashi J Reprod Dev Original Article The present study investigated the effect of autophagy induction and cathepsin B (CTSB) inhibition on developmental competence of poor quality oocytes. Bovine cumulus oocyte complexes (COCs) were classified as good or poor according to their morphology. Autophagy activity was detected in good and poor germinal vesicle (GV) oocytes. Then E-64, a CTSB inhibitor, rapamycin (Rapa), an autophagy inducer, and combined administration was achieved during in vitro maturation (IVM) of poor quality COCs followed by detection of autophagy activity. In the next experiment, E-64, Rapa, and E64 + Rapa, were added during IVM to good and poor quality COCs followed by in vitro fertilization and culture for 8 days to investigate whether inhibition of CTSB and/or induction of autophagy improve embryonic development and quality. Autophagy activity was significantly lower in poor quality GV oocytes than in good quality ones. E-64, Rapa and E-64 + Rapa treatment during IVM significantly increased autophagy activity in poor quality oocytes. Addition of Rapa in good quality COCs did not increase the blastocyst rate, whereas E-64 increased the blastocyst rate and total cell number (TCN) with decreasing TUNEL-positive cells. In contrast, Rapa treatment in poor quality COCs significantly increased the blastocyst rate and TCN with decreasing TUNEL-positive cells. These results indicate oocyte quality has different responses to intracellular autophagy induction and CTSB activity control by potential autophagy and catabolic status, however, synergetic effect of autophagy induction and CTSB inhibition can increase developmental competence of both good and poor quality COCs, especially rescue effect in poor quality COCs. The Society for Reproduction and Development 2019-12-25 2020-02 /pmc/articles/PMC7040212/ /pubmed/31875588 http://dx.doi.org/10.1262/jrd.2019-123 Text en ©2020 Society for Reproduction and Development This is an open-access article distributed under the terms of the Creative Commons Attribution Non-Commercial No Derivatives (by-nc-nd) License. (CC-BY-NC-ND 4.0: https://creativecommons.org/licenses/by-nc-nd/4.0/)
spellingShingle Original Article
LI, Jianye
BALBOULA, Ahmed Zaky
ABOELENAIN, Mansour
FUJII, Takashi
MORIYASU, Satoru
BAI, Hanako
KAWAHARA, Manabu
TAKAHASHI, Masashi
Effect of autophagy induction and cathepsin B inhibition on developmental competence of poor quality bovine oocytes
title Effect of autophagy induction and cathepsin B inhibition on developmental competence of poor quality bovine oocytes
title_full Effect of autophagy induction and cathepsin B inhibition on developmental competence of poor quality bovine oocytes
title_fullStr Effect of autophagy induction and cathepsin B inhibition on developmental competence of poor quality bovine oocytes
title_full_unstemmed Effect of autophagy induction and cathepsin B inhibition on developmental competence of poor quality bovine oocytes
title_short Effect of autophagy induction and cathepsin B inhibition on developmental competence of poor quality bovine oocytes
title_sort effect of autophagy induction and cathepsin b inhibition on developmental competence of poor quality bovine oocytes
topic Original Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7040212/
https://www.ncbi.nlm.nih.gov/pubmed/31875588
http://dx.doi.org/10.1262/jrd.2019-123
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