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Suppression of mosaic mutation by co-delivery of CRISPR associated protein 9 and three-prime repair exonuclease 2 into porcine zygotes via electroporation
Gene-modified animals, including pigs, can be generated efficiently by introducing CRISPR associated protein 9 (CRISPR/Cas9) into zygotes. However, in many cases, these zygotes tend to become mosaic mutants with various different mutant cell types, making it difficult to analyze the phenotype of gen...
Autores principales: | YAMASHITA, Shiro, KOGASAKA, Yuhei, HIRADATE, Yuuki, TANEMURA, Kentaro, SENDAI, Yutaka |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
The Society for Reproduction and Development
2019
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7040215/ https://www.ncbi.nlm.nih.gov/pubmed/31761839 http://dx.doi.org/10.1262/jrd.2019-088 |
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