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Exploration of hypoglycemic effect of an extract from leaves of a plant from Tunisian pharmacopeia: Artemisia campestris (Asteraceae)
BACKGROUND AND OBJECTIVES: A lot of research has been directed towards medicinal plants which are considered as a source of multiple phytotherapic substances endowed with hypoglycemic activities that could be used to treat diabetes and its complications. Our study was carried out in Wistar rats to i...
Autores principales: | , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Makerere Medical School
2019
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7040308/ https://www.ncbi.nlm.nih.gov/pubmed/32127860 http://dx.doi.org/10.4314/ahs.v19i4.6 |
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author | Belgacem, Amel Gdara, Neyla Ben Khemiri, Ikram Bitri, Lotfi |
author_facet | Belgacem, Amel Gdara, Neyla Ben Khemiri, Ikram Bitri, Lotfi |
author_sort | Belgacem, Amel |
collection | PubMed |
description | BACKGROUND AND OBJECTIVES: A lot of research has been directed towards medicinal plants which are considered as a source of multiple phytotherapic substances endowed with hypoglycemic activities that could be used to treat diabetes and its complications. Our study was carried out in Wistar rats to investigate the hypoglycemic effect of n-Butanol Fraction from Artemisia campestris leaf Methanolic Extract (BFACME). METHODS: Two experimental models were used in rats: orally induced hyperglycemia (OGTT) and isolated perfused liver (IPRL). RESULTS: BFACME at 550 mg/kg BW dose significantly reduced fasting glucose level in normal rats as compared to controls. The decrease of glycaemia was 12.6% more significant than that obtained with the standard drug glibenclamide (10 mg/kg BW), an oral antidiabetic preparation belonging to sulfonylurea class. In OGTT model, BFACME at the highest doses of 550 and 400 mg/kg BW significantly reduced the postprandial hyperglycemic peak compared to controls. In the IPRL model, treatment with BFACME significantly decreased glucose concentrations after 30 min of perfusion with 30 mM glucose solely when insulin was present. The higher doses of BFACME lead to glucose concentration at basal level as early as 90 min, while the lowest dose does not restore this concentration even to t = 120min. The best initial glucose concentration retrieval was obtained with 0.7 mg BFACME/mL/g liver. At this dose, BFACME improves the decrease of glucose level caused by only insulin by about 18%. CONCLUSION: The BFACME appears to exert a hypoglycemic activity by potentiating the insulin action. |
format | Online Article Text |
id | pubmed-7040308 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2019 |
publisher | Makerere Medical School |
record_format | MEDLINE/PubMed |
spelling | pubmed-70403082020-03-03 Exploration of hypoglycemic effect of an extract from leaves of a plant from Tunisian pharmacopeia: Artemisia campestris (Asteraceae) Belgacem, Amel Gdara, Neyla Ben Khemiri, Ikram Bitri, Lotfi Afr Health Sci Articles BACKGROUND AND OBJECTIVES: A lot of research has been directed towards medicinal plants which are considered as a source of multiple phytotherapic substances endowed with hypoglycemic activities that could be used to treat diabetes and its complications. Our study was carried out in Wistar rats to investigate the hypoglycemic effect of n-Butanol Fraction from Artemisia campestris leaf Methanolic Extract (BFACME). METHODS: Two experimental models were used in rats: orally induced hyperglycemia (OGTT) and isolated perfused liver (IPRL). RESULTS: BFACME at 550 mg/kg BW dose significantly reduced fasting glucose level in normal rats as compared to controls. The decrease of glycaemia was 12.6% more significant than that obtained with the standard drug glibenclamide (10 mg/kg BW), an oral antidiabetic preparation belonging to sulfonylurea class. In OGTT model, BFACME at the highest doses of 550 and 400 mg/kg BW significantly reduced the postprandial hyperglycemic peak compared to controls. In the IPRL model, treatment with BFACME significantly decreased glucose concentrations after 30 min of perfusion with 30 mM glucose solely when insulin was present. The higher doses of BFACME lead to glucose concentration at basal level as early as 90 min, while the lowest dose does not restore this concentration even to t = 120min. The best initial glucose concentration retrieval was obtained with 0.7 mg BFACME/mL/g liver. At this dose, BFACME improves the decrease of glucose level caused by only insulin by about 18%. CONCLUSION: The BFACME appears to exert a hypoglycemic activity by potentiating the insulin action. Makerere Medical School 2019-12 /pmc/articles/PMC7040308/ /pubmed/32127860 http://dx.doi.org/10.4314/ahs.v19i4.6 Text en © 2019 Belgacem et al. Licensee African Health Sciences. This is an Open Access article distributed under the terms of the Creative commons Attribution License (https://creativecommons.org/licenses/BY/4.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. |
spellingShingle | Articles Belgacem, Amel Gdara, Neyla Ben Khemiri, Ikram Bitri, Lotfi Exploration of hypoglycemic effect of an extract from leaves of a plant from Tunisian pharmacopeia: Artemisia campestris (Asteraceae) |
title | Exploration of hypoglycemic effect of an extract from leaves of a plant from Tunisian pharmacopeia: Artemisia campestris (Asteraceae) |
title_full | Exploration of hypoglycemic effect of an extract from leaves of a plant from Tunisian pharmacopeia: Artemisia campestris (Asteraceae) |
title_fullStr | Exploration of hypoglycemic effect of an extract from leaves of a plant from Tunisian pharmacopeia: Artemisia campestris (Asteraceae) |
title_full_unstemmed | Exploration of hypoglycemic effect of an extract from leaves of a plant from Tunisian pharmacopeia: Artemisia campestris (Asteraceae) |
title_short | Exploration of hypoglycemic effect of an extract from leaves of a plant from Tunisian pharmacopeia: Artemisia campestris (Asteraceae) |
title_sort | exploration of hypoglycemic effect of an extract from leaves of a plant from tunisian pharmacopeia: artemisia campestris (asteraceae) |
topic | Articles |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7040308/ https://www.ncbi.nlm.nih.gov/pubmed/32127860 http://dx.doi.org/10.4314/ahs.v19i4.6 |
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