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Determination of Chitin Content in Insects: An Alternate Method Based on Calcofluor Staining
Chitin is an aminopolysaccharide present in yeast cells and arthropod cuticle and is one of the most abundant biopolymers. The conventional methods for the quantitation of chitin content in biological samples are based on its hydrolysis (acid or enzymatic), and the assessment of the byproduct, gluco...
Autores principales: | , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Frontiers Media S.A.
2020
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7040371/ https://www.ncbi.nlm.nih.gov/pubmed/32132935 http://dx.doi.org/10.3389/fphys.2020.00117 |