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Dual Imaging Gold Nanoplatforms for Targeted Radiotheranostics
Gold nanoparticles (AuNPs) are interesting for the design of new cancer theranostic tools, mainly due to their biocompatibility, easy molecular vectorization, and good biological half-life. Herein, we report a gold nanoparticle platform as a bimodal imaging probe, capable of coordinating Gd(3+) for...
Autores principales: | , , , , , , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
MDPI
2020
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7040626/ https://www.ncbi.nlm.nih.gov/pubmed/31978954 http://dx.doi.org/10.3390/ma13030513 |
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author | Silva, Francisco Paulo, António Pallier, Agnès Même, Sandra Tóth, Éva Gano, Lurdes Marques, Fernanda Geraldes, Carlos F.G.C. Castro, M. Margarida C.A. Cardoso, Ana M. Jurado, Amália S. López-Larrubia, Pilar Lacerda, Sara Cabral Campello, Maria Paula |
author_facet | Silva, Francisco Paulo, António Pallier, Agnès Même, Sandra Tóth, Éva Gano, Lurdes Marques, Fernanda Geraldes, Carlos F.G.C. Castro, M. Margarida C.A. Cardoso, Ana M. Jurado, Amália S. López-Larrubia, Pilar Lacerda, Sara Cabral Campello, Maria Paula |
author_sort | Silva, Francisco |
collection | PubMed |
description | Gold nanoparticles (AuNPs) are interesting for the design of new cancer theranostic tools, mainly due to their biocompatibility, easy molecular vectorization, and good biological half-life. Herein, we report a gold nanoparticle platform as a bimodal imaging probe, capable of coordinating Gd(3+) for Magnetic Resonance Imaging (MRI) and (67)Ga(3+) for Single Photon Emission Computed Tomography (SPECT) imaging. Our AuNPs carry a bombesin analogue with affinity towards the gastrin releasing peptide receptor (GRPr), overexpressed in a variety of human cancer cells, namely PC3 prostate cancer cells. The potential of these multimodal imaging nanoconstructs was thoroughly investigated by the assessment of their magnetic properties, in vitro cellular uptake, biodistribution, and radiosensitisation assays. The relaxometric properties predict a potential T(1)- and T(2)- MRI application. The promising in vitro cellular uptake of (67)Ga/Gd-based bombesin containing particles was confirmed through biodistribution studies in tumor bearing mice, indicating their integrity and ability to target the GRPr. Radiosensitization studies revealed the therapeutic potential of the nanoparticles. Moreover, the DOTA chelating unit moiety versatility gives a high theranostic potential through the coordination of other therapeutically interesting radiometals. Altogether, our nanoparticles are interesting nanomaterial for theranostic application and as bimodal T(1)- and T(2)- MRI / SPECT imaging probes. |
format | Online Article Text |
id | pubmed-7040626 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2020 |
publisher | MDPI |
record_format | MEDLINE/PubMed |
spelling | pubmed-70406262020-03-09 Dual Imaging Gold Nanoplatforms for Targeted Radiotheranostics Silva, Francisco Paulo, António Pallier, Agnès Même, Sandra Tóth, Éva Gano, Lurdes Marques, Fernanda Geraldes, Carlos F.G.C. Castro, M. Margarida C.A. Cardoso, Ana M. Jurado, Amália S. López-Larrubia, Pilar Lacerda, Sara Cabral Campello, Maria Paula Materials (Basel) Article Gold nanoparticles (AuNPs) are interesting for the design of new cancer theranostic tools, mainly due to their biocompatibility, easy molecular vectorization, and good biological half-life. Herein, we report a gold nanoparticle platform as a bimodal imaging probe, capable of coordinating Gd(3+) for Magnetic Resonance Imaging (MRI) and (67)Ga(3+) for Single Photon Emission Computed Tomography (SPECT) imaging. Our AuNPs carry a bombesin analogue with affinity towards the gastrin releasing peptide receptor (GRPr), overexpressed in a variety of human cancer cells, namely PC3 prostate cancer cells. The potential of these multimodal imaging nanoconstructs was thoroughly investigated by the assessment of their magnetic properties, in vitro cellular uptake, biodistribution, and radiosensitisation assays. The relaxometric properties predict a potential T(1)- and T(2)- MRI application. The promising in vitro cellular uptake of (67)Ga/Gd-based bombesin containing particles was confirmed through biodistribution studies in tumor bearing mice, indicating their integrity and ability to target the GRPr. Radiosensitization studies revealed the therapeutic potential of the nanoparticles. Moreover, the DOTA chelating unit moiety versatility gives a high theranostic potential through the coordination of other therapeutically interesting radiometals. Altogether, our nanoparticles are interesting nanomaterial for theranostic application and as bimodal T(1)- and T(2)- MRI / SPECT imaging probes. MDPI 2020-01-22 /pmc/articles/PMC7040626/ /pubmed/31978954 http://dx.doi.org/10.3390/ma13030513 Text en © 2020 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/). |
spellingShingle | Article Silva, Francisco Paulo, António Pallier, Agnès Même, Sandra Tóth, Éva Gano, Lurdes Marques, Fernanda Geraldes, Carlos F.G.C. Castro, M. Margarida C.A. Cardoso, Ana M. Jurado, Amália S. López-Larrubia, Pilar Lacerda, Sara Cabral Campello, Maria Paula Dual Imaging Gold Nanoplatforms for Targeted Radiotheranostics |
title | Dual Imaging Gold Nanoplatforms for Targeted Radiotheranostics |
title_full | Dual Imaging Gold Nanoplatforms for Targeted Radiotheranostics |
title_fullStr | Dual Imaging Gold Nanoplatforms for Targeted Radiotheranostics |
title_full_unstemmed | Dual Imaging Gold Nanoplatforms for Targeted Radiotheranostics |
title_short | Dual Imaging Gold Nanoplatforms for Targeted Radiotheranostics |
title_sort | dual imaging gold nanoplatforms for targeted radiotheranostics |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7040626/ https://www.ncbi.nlm.nih.gov/pubmed/31978954 http://dx.doi.org/10.3390/ma13030513 |
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