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Dual Imaging Gold Nanoplatforms for Targeted Radiotheranostics

Gold nanoparticles (AuNPs) are interesting for the design of new cancer theranostic tools, mainly due to their biocompatibility, easy molecular vectorization, and good biological half-life. Herein, we report a gold nanoparticle platform as a bimodal imaging probe, capable of coordinating Gd(3+) for...

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Autores principales: Silva, Francisco, Paulo, António, Pallier, Agnès, Même, Sandra, Tóth, Éva, Gano, Lurdes, Marques, Fernanda, Geraldes, Carlos F.G.C., Castro, M. Margarida C.A., Cardoso, Ana M., Jurado, Amália S., López-Larrubia, Pilar, Lacerda, Sara, Cabral Campello, Maria Paula
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2020
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7040626/
https://www.ncbi.nlm.nih.gov/pubmed/31978954
http://dx.doi.org/10.3390/ma13030513
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author Silva, Francisco
Paulo, António
Pallier, Agnès
Même, Sandra
Tóth, Éva
Gano, Lurdes
Marques, Fernanda
Geraldes, Carlos F.G.C.
Castro, M. Margarida C.A.
Cardoso, Ana M.
Jurado, Amália S.
López-Larrubia, Pilar
Lacerda, Sara
Cabral Campello, Maria Paula
author_facet Silva, Francisco
Paulo, António
Pallier, Agnès
Même, Sandra
Tóth, Éva
Gano, Lurdes
Marques, Fernanda
Geraldes, Carlos F.G.C.
Castro, M. Margarida C.A.
Cardoso, Ana M.
Jurado, Amália S.
López-Larrubia, Pilar
Lacerda, Sara
Cabral Campello, Maria Paula
author_sort Silva, Francisco
collection PubMed
description Gold nanoparticles (AuNPs) are interesting for the design of new cancer theranostic tools, mainly due to their biocompatibility, easy molecular vectorization, and good biological half-life. Herein, we report a gold nanoparticle platform as a bimodal imaging probe, capable of coordinating Gd(3+) for Magnetic Resonance Imaging (MRI) and (67)Ga(3+) for Single Photon Emission Computed Tomography (SPECT) imaging. Our AuNPs carry a bombesin analogue with affinity towards the gastrin releasing peptide receptor (GRPr), overexpressed in a variety of human cancer cells, namely PC3 prostate cancer cells. The potential of these multimodal imaging nanoconstructs was thoroughly investigated by the assessment of their magnetic properties, in vitro cellular uptake, biodistribution, and radiosensitisation assays. The relaxometric properties predict a potential T(1)- and T(2)- MRI application. The promising in vitro cellular uptake of (67)Ga/Gd-based bombesin containing particles was confirmed through biodistribution studies in tumor bearing mice, indicating their integrity and ability to target the GRPr. Radiosensitization studies revealed the therapeutic potential of the nanoparticles. Moreover, the DOTA chelating unit moiety versatility gives a high theranostic potential through the coordination of other therapeutically interesting radiometals. Altogether, our nanoparticles are interesting nanomaterial for theranostic application and as bimodal T(1)- and T(2)- MRI / SPECT imaging probes.
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spelling pubmed-70406262020-03-09 Dual Imaging Gold Nanoplatforms for Targeted Radiotheranostics Silva, Francisco Paulo, António Pallier, Agnès Même, Sandra Tóth, Éva Gano, Lurdes Marques, Fernanda Geraldes, Carlos F.G.C. Castro, M. Margarida C.A. Cardoso, Ana M. Jurado, Amália S. López-Larrubia, Pilar Lacerda, Sara Cabral Campello, Maria Paula Materials (Basel) Article Gold nanoparticles (AuNPs) are interesting for the design of new cancer theranostic tools, mainly due to their biocompatibility, easy molecular vectorization, and good biological half-life. Herein, we report a gold nanoparticle platform as a bimodal imaging probe, capable of coordinating Gd(3+) for Magnetic Resonance Imaging (MRI) and (67)Ga(3+) for Single Photon Emission Computed Tomography (SPECT) imaging. Our AuNPs carry a bombesin analogue with affinity towards the gastrin releasing peptide receptor (GRPr), overexpressed in a variety of human cancer cells, namely PC3 prostate cancer cells. The potential of these multimodal imaging nanoconstructs was thoroughly investigated by the assessment of their magnetic properties, in vitro cellular uptake, biodistribution, and radiosensitisation assays. The relaxometric properties predict a potential T(1)- and T(2)- MRI application. The promising in vitro cellular uptake of (67)Ga/Gd-based bombesin containing particles was confirmed through biodistribution studies in tumor bearing mice, indicating their integrity and ability to target the GRPr. Radiosensitization studies revealed the therapeutic potential of the nanoparticles. Moreover, the DOTA chelating unit moiety versatility gives a high theranostic potential through the coordination of other therapeutically interesting radiometals. Altogether, our nanoparticles are interesting nanomaterial for theranostic application and as bimodal T(1)- and T(2)- MRI / SPECT imaging probes. MDPI 2020-01-22 /pmc/articles/PMC7040626/ /pubmed/31978954 http://dx.doi.org/10.3390/ma13030513 Text en © 2020 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Silva, Francisco
Paulo, António
Pallier, Agnès
Même, Sandra
Tóth, Éva
Gano, Lurdes
Marques, Fernanda
Geraldes, Carlos F.G.C.
Castro, M. Margarida C.A.
Cardoso, Ana M.
Jurado, Amália S.
López-Larrubia, Pilar
Lacerda, Sara
Cabral Campello, Maria Paula
Dual Imaging Gold Nanoplatforms for Targeted Radiotheranostics
title Dual Imaging Gold Nanoplatforms for Targeted Radiotheranostics
title_full Dual Imaging Gold Nanoplatforms for Targeted Radiotheranostics
title_fullStr Dual Imaging Gold Nanoplatforms for Targeted Radiotheranostics
title_full_unstemmed Dual Imaging Gold Nanoplatforms for Targeted Radiotheranostics
title_short Dual Imaging Gold Nanoplatforms for Targeted Radiotheranostics
title_sort dual imaging gold nanoplatforms for targeted radiotheranostics
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7040626/
https://www.ncbi.nlm.nih.gov/pubmed/31978954
http://dx.doi.org/10.3390/ma13030513
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