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Er,Cr:YSGG Laser Performance Improves Biological Response on Titanium Surfaces

Porphyromonas gingivalis infection is one of the causes of implant failures, which can lead to peri-implantitis. Implant surface roughness is reportedly related strongly to P. gingivalis adhesion, which can lead to peri-implantitis and, later, cell adhesion. Our aim was to evaluate the effects of Er...

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Autores principales: Yao, Wan-Ling, Lin, Jerry Chin Yi, Salamanca, Eisner, Pan, Yu-Hwa, Tsai, Pei-Yo, Leu, Sy-Jye, Yang, Kai-Chiang, Huang, Haw-Ming, Huang, Huei-Yu, Chang, Wei-Jen
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2020
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7040680/
https://www.ncbi.nlm.nih.gov/pubmed/32046015
http://dx.doi.org/10.3390/ma13030756
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author Yao, Wan-Ling
Lin, Jerry Chin Yi
Salamanca, Eisner
Pan, Yu-Hwa
Tsai, Pei-Yo
Leu, Sy-Jye
Yang, Kai-Chiang
Huang, Haw-Ming
Huang, Huei-Yu
Chang, Wei-Jen
author_facet Yao, Wan-Ling
Lin, Jerry Chin Yi
Salamanca, Eisner
Pan, Yu-Hwa
Tsai, Pei-Yo
Leu, Sy-Jye
Yang, Kai-Chiang
Huang, Haw-Ming
Huang, Huei-Yu
Chang, Wei-Jen
author_sort Yao, Wan-Ling
collection PubMed
description Porphyromonas gingivalis infection is one of the causes of implant failures, which can lead to peri-implantitis. Implant surface roughness is reportedly related strongly to P. gingivalis adhesion, which can lead to peri-implantitis and, later, cell adhesion. Our aim was to evaluate the effects of Er,Cr:YSGG laser on titanium (Ti) disc surfaces and its interaction with bacterial adhesion and fibroblast viability. Ti discs underwent two treatments: autoclaving (control) and erbium, chromium-doped yttrium scandium gallium garnet (Er,Cr:YSGG) laser treatment (test). Ti disc surfaces were examined with scanning electronic microscope (SEM), Energy-dispersive spectrometry (EDX), X-ray photoelectron spectroscopy (XPS). The surface roughness same as wettability were also investigated. Fibroblast viability was assessed with the water-soluble tetrazolium 1 (WST-1) test, and osteoblast differentiation was assessed with the alkaline phosphatase (ALP) assay. Bacterial structure and colony formation were detected with scanning electron microscopy and Gram stain. In comparison to control discs, the test discs showed smoother surfaces, with 0.25-µm decrease in surface roughness (p < 0.05); lower P. gingivalis adhesion (p < 0.01); less P. gingivalis colonization (p < 0.05); and increased fibroblast viability and osteoblast differentiation (p < 0.05). Er,Cr:YSGG laser treatment improved disc surfaces by making them slightly smoother, which reduced P. gingivalis adhesion and increased fibroblast viability and osteoblast differentiation. Er,Cr:YSGG laser treatment can be considered a good option for managing peri-implantitis. Further investigations of laser-assisted therapy are necessary for better guidelines in the treatment of peri-implantitis.
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spelling pubmed-70406802020-03-09 Er,Cr:YSGG Laser Performance Improves Biological Response on Titanium Surfaces Yao, Wan-Ling Lin, Jerry Chin Yi Salamanca, Eisner Pan, Yu-Hwa Tsai, Pei-Yo Leu, Sy-Jye Yang, Kai-Chiang Huang, Haw-Ming Huang, Huei-Yu Chang, Wei-Jen Materials (Basel) Article Porphyromonas gingivalis infection is one of the causes of implant failures, which can lead to peri-implantitis. Implant surface roughness is reportedly related strongly to P. gingivalis adhesion, which can lead to peri-implantitis and, later, cell adhesion. Our aim was to evaluate the effects of Er,Cr:YSGG laser on titanium (Ti) disc surfaces and its interaction with bacterial adhesion and fibroblast viability. Ti discs underwent two treatments: autoclaving (control) and erbium, chromium-doped yttrium scandium gallium garnet (Er,Cr:YSGG) laser treatment (test). Ti disc surfaces were examined with scanning electronic microscope (SEM), Energy-dispersive spectrometry (EDX), X-ray photoelectron spectroscopy (XPS). The surface roughness same as wettability were also investigated. Fibroblast viability was assessed with the water-soluble tetrazolium 1 (WST-1) test, and osteoblast differentiation was assessed with the alkaline phosphatase (ALP) assay. Bacterial structure and colony formation were detected with scanning electron microscopy and Gram stain. In comparison to control discs, the test discs showed smoother surfaces, with 0.25-µm decrease in surface roughness (p < 0.05); lower P. gingivalis adhesion (p < 0.01); less P. gingivalis colonization (p < 0.05); and increased fibroblast viability and osteoblast differentiation (p < 0.05). Er,Cr:YSGG laser treatment improved disc surfaces by making them slightly smoother, which reduced P. gingivalis adhesion and increased fibroblast viability and osteoblast differentiation. Er,Cr:YSGG laser treatment can be considered a good option for managing peri-implantitis. Further investigations of laser-assisted therapy are necessary for better guidelines in the treatment of peri-implantitis. MDPI 2020-02-07 /pmc/articles/PMC7040680/ /pubmed/32046015 http://dx.doi.org/10.3390/ma13030756 Text en © 2020 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Yao, Wan-Ling
Lin, Jerry Chin Yi
Salamanca, Eisner
Pan, Yu-Hwa
Tsai, Pei-Yo
Leu, Sy-Jye
Yang, Kai-Chiang
Huang, Haw-Ming
Huang, Huei-Yu
Chang, Wei-Jen
Er,Cr:YSGG Laser Performance Improves Biological Response on Titanium Surfaces
title Er,Cr:YSGG Laser Performance Improves Biological Response on Titanium Surfaces
title_full Er,Cr:YSGG Laser Performance Improves Biological Response on Titanium Surfaces
title_fullStr Er,Cr:YSGG Laser Performance Improves Biological Response on Titanium Surfaces
title_full_unstemmed Er,Cr:YSGG Laser Performance Improves Biological Response on Titanium Surfaces
title_short Er,Cr:YSGG Laser Performance Improves Biological Response on Titanium Surfaces
title_sort er,cr:ysgg laser performance improves biological response on titanium surfaces
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7040680/
https://www.ncbi.nlm.nih.gov/pubmed/32046015
http://dx.doi.org/10.3390/ma13030756
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