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Evaluation of the Use of an Inorganic Bone Matrix in the Repair of Bone Defects in Rats Submitted to Experimental Alcoholism

To assess the effects of chronic alcoholism on the repair of bone defects associated with xenograft. Forty male rats were distributed in: control group (CG, n = 20) and experimental group (EG, n = 20), which received 25% ethanol ad libitum after a period of adaptation. After 90 days of liquid diet,...

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Autores principales: Santos German, Iris Jasmin, Pomini, Karina Torres, Bighetti, Ana Carolina Cestari, Andreo, Jesus Carlos, Reis, Carlos Henrique Bertoni, Shinohara, André Luis, Rosa Júnior, Geraldo Marco, Teixeira, Daniel de Bortoli, Rosso, Marcelie Priscila de Oliveira, Buchaim, Daniela Vieira, Buchaim, Rogério Leone
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2020
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7040897/
https://www.ncbi.nlm.nih.gov/pubmed/32033088
http://dx.doi.org/10.3390/ma13030695
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author Santos German, Iris Jasmin
Pomini, Karina Torres
Bighetti, Ana Carolina Cestari
Andreo, Jesus Carlos
Reis, Carlos Henrique Bertoni
Shinohara, André Luis
Rosa Júnior, Geraldo Marco
Teixeira, Daniel de Bortoli
Rosso, Marcelie Priscila de Oliveira
Buchaim, Daniela Vieira
Buchaim, Rogério Leone
author_facet Santos German, Iris Jasmin
Pomini, Karina Torres
Bighetti, Ana Carolina Cestari
Andreo, Jesus Carlos
Reis, Carlos Henrique Bertoni
Shinohara, André Luis
Rosa Júnior, Geraldo Marco
Teixeira, Daniel de Bortoli
Rosso, Marcelie Priscila de Oliveira
Buchaim, Daniela Vieira
Buchaim, Rogério Leone
author_sort Santos German, Iris Jasmin
collection PubMed
description To assess the effects of chronic alcoholism on the repair of bone defects associated with xenograft. Forty male rats were distributed in: control group (CG, n = 20) and experimental group (EG, n = 20), which received 25% ethanol ad libitum after a period of adaptation. After 90 days of liquid diet, the rats were submitted to 5.0-mm bilateral craniotomy on the parietal bones, subdividing into groups: CCG (control group that received only water with liquid diet and the defect was filled with blood clot), BCG (control group that received only water with liquid diet and the defect was filled with biomaterial), CEG (alcoholic group that received only ethanol solution 25% v/v with liquid diet and the defect was filled with blood clot), and BEG (alcoholic group that received only ethanol solution 25% v/v with liquid diet and the defect was filled with biomaterial). In the analysis of body mass, the drunk animals presented the lowest averages in relation to non-drunk animals during the experimental period. Histomorphologically all groups presented bone formation restricted to the defect margins at 60 days, with bone islets adjacent to the BCG biomaterial particles. CEG showed significant difference compared to BEG only at 40 days (17.42 ± 2.78 vs. 9.59 ± 4.59, respectively). In the birefringence analysis, in early periods all groups showed red-orange birefringence turning greenish-yellow at the end of the experiment. The results provided that, regardless of clinical condition, i.e., alcoholic or non-alcoholic, in the final period of the experiment, the process of bone defect recomposition was similar with the use of xenograft or only clot.
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spelling pubmed-70408972020-03-09 Evaluation of the Use of an Inorganic Bone Matrix in the Repair of Bone Defects in Rats Submitted to Experimental Alcoholism Santos German, Iris Jasmin Pomini, Karina Torres Bighetti, Ana Carolina Cestari Andreo, Jesus Carlos Reis, Carlos Henrique Bertoni Shinohara, André Luis Rosa Júnior, Geraldo Marco Teixeira, Daniel de Bortoli Rosso, Marcelie Priscila de Oliveira Buchaim, Daniela Vieira Buchaim, Rogério Leone Materials (Basel) Article To assess the effects of chronic alcoholism on the repair of bone defects associated with xenograft. Forty male rats were distributed in: control group (CG, n = 20) and experimental group (EG, n = 20), which received 25% ethanol ad libitum after a period of adaptation. After 90 days of liquid diet, the rats were submitted to 5.0-mm bilateral craniotomy on the parietal bones, subdividing into groups: CCG (control group that received only water with liquid diet and the defect was filled with blood clot), BCG (control group that received only water with liquid diet and the defect was filled with biomaterial), CEG (alcoholic group that received only ethanol solution 25% v/v with liquid diet and the defect was filled with blood clot), and BEG (alcoholic group that received only ethanol solution 25% v/v with liquid diet and the defect was filled with biomaterial). In the analysis of body mass, the drunk animals presented the lowest averages in relation to non-drunk animals during the experimental period. Histomorphologically all groups presented bone formation restricted to the defect margins at 60 days, with bone islets adjacent to the BCG biomaterial particles. CEG showed significant difference compared to BEG only at 40 days (17.42 ± 2.78 vs. 9.59 ± 4.59, respectively). In the birefringence analysis, in early periods all groups showed red-orange birefringence turning greenish-yellow at the end of the experiment. The results provided that, regardless of clinical condition, i.e., alcoholic or non-alcoholic, in the final period of the experiment, the process of bone defect recomposition was similar with the use of xenograft or only clot. MDPI 2020-02-04 /pmc/articles/PMC7040897/ /pubmed/32033088 http://dx.doi.org/10.3390/ma13030695 Text en © 2020 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Santos German, Iris Jasmin
Pomini, Karina Torres
Bighetti, Ana Carolina Cestari
Andreo, Jesus Carlos
Reis, Carlos Henrique Bertoni
Shinohara, André Luis
Rosa Júnior, Geraldo Marco
Teixeira, Daniel de Bortoli
Rosso, Marcelie Priscila de Oliveira
Buchaim, Daniela Vieira
Buchaim, Rogério Leone
Evaluation of the Use of an Inorganic Bone Matrix in the Repair of Bone Defects in Rats Submitted to Experimental Alcoholism
title Evaluation of the Use of an Inorganic Bone Matrix in the Repair of Bone Defects in Rats Submitted to Experimental Alcoholism
title_full Evaluation of the Use of an Inorganic Bone Matrix in the Repair of Bone Defects in Rats Submitted to Experimental Alcoholism
title_fullStr Evaluation of the Use of an Inorganic Bone Matrix in the Repair of Bone Defects in Rats Submitted to Experimental Alcoholism
title_full_unstemmed Evaluation of the Use of an Inorganic Bone Matrix in the Repair of Bone Defects in Rats Submitted to Experimental Alcoholism
title_short Evaluation of the Use of an Inorganic Bone Matrix in the Repair of Bone Defects in Rats Submitted to Experimental Alcoholism
title_sort evaluation of the use of an inorganic bone matrix in the repair of bone defects in rats submitted to experimental alcoholism
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7040897/
https://www.ncbi.nlm.nih.gov/pubmed/32033088
http://dx.doi.org/10.3390/ma13030695
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