Cargando…
Identification of novel influenza A virus exposures by an improved high‐throughput multiplex MAGPIX platform and serum adsorption
BACKGROUND: The development of serologic assays that can rapidly assess human exposure to novel influenza viruses remains a public health need. Previously, we developed an 11‐plex magnetic fluorescence microsphere immunoassay (MAGPIX) by using globular head domain recombinant hemagglutinins (rHAs) w...
Autores principales: | , , , , , , , , , , , , |
---|---|
Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
John Wiley and Sons Inc.
2019
|
Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7040970/ https://www.ncbi.nlm.nih.gov/pubmed/31701647 http://dx.doi.org/10.1111/irv.12695 |
_version_ | 1783501097659269120 |
---|---|
author | Li, Zhu‐Nan Cheng, Emily Poirot, Eugenie Weber, Kimberly M. Carney, Paul Chang, Jessie Liu, Feng Gross, F. Liaini Holiday, Crystal Fry, Alicia Stevens, James Tumpey, Terrence Levine, Min Z. |
author_facet | Li, Zhu‐Nan Cheng, Emily Poirot, Eugenie Weber, Kimberly M. Carney, Paul Chang, Jessie Liu, Feng Gross, F. Liaini Holiday, Crystal Fry, Alicia Stevens, James Tumpey, Terrence Levine, Min Z. |
author_sort | Li, Zhu‐Nan |
collection | PubMed |
description | BACKGROUND: The development of serologic assays that can rapidly assess human exposure to novel influenza viruses remains a public health need. Previously, we developed an 11‐plex magnetic fluorescence microsphere immunoassay (MAGPIX) by using globular head domain recombinant hemagglutinins (rHAs) with serum adsorption using two ectodomain rHAs. METHODS: We compared sera collected from two cohorts with novel influenza exposures: animal shelter staff during an A(H7N2) outbreak in New York City in 2016‐2017 (n = 119 single sera) and poultry workers from a live bird market in Bangladesh in 2012‐2014 (n = 29 pairs). Sera were analyzed by microneutralization (MN) assay and a 20‐plex MAGPIX assay with rHAs from 19 influenza strains (11 subtypes) combined with serum adsorption using 8 rHAs from A(H1N1) and A(H3N2) viruses. Antibody responses were analyzed to determine the novel influenza virus exposure. RESULTS: Among persons with novel influenza virus exposures, the median fluorescence intensity (MFI) against the novel rHA from exposed influenza virus had the highest correlation with MN titers to the same viruses and could be confirmed by removal of cross‐reactivity from seasonal H1/H3 rHAs following serum adsorption. Interestingly, in persons with exposures to novel influenza viruses, age and MFIs against exposed novel HA were negatively correlated, whereas in persons without exposure to novel influenza viruses, age and MFI against novel HAs were positively correlated. CONCLUSIONS: This 20‐plex high‐throughput assay with serum adsorption will be a useful tool to detect novel influenza virus infections during influenza outbreak investigations and surveillance, especially when well‐paired serum samples are not available. |
format | Online Article Text |
id | pubmed-7040970 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2019 |
publisher | John Wiley and Sons Inc. |
record_format | MEDLINE/PubMed |
spelling | pubmed-70409702020-03-01 Identification of novel influenza A virus exposures by an improved high‐throughput multiplex MAGPIX platform and serum adsorption Li, Zhu‐Nan Cheng, Emily Poirot, Eugenie Weber, Kimberly M. Carney, Paul Chang, Jessie Liu, Feng Gross, F. Liaini Holiday, Crystal Fry, Alicia Stevens, James Tumpey, Terrence Levine, Min Z. Influenza Other Respir Viruses Original Articles BACKGROUND: The development of serologic assays that can rapidly assess human exposure to novel influenza viruses remains a public health need. Previously, we developed an 11‐plex magnetic fluorescence microsphere immunoassay (MAGPIX) by using globular head domain recombinant hemagglutinins (rHAs) with serum adsorption using two ectodomain rHAs. METHODS: We compared sera collected from two cohorts with novel influenza exposures: animal shelter staff during an A(H7N2) outbreak in New York City in 2016‐2017 (n = 119 single sera) and poultry workers from a live bird market in Bangladesh in 2012‐2014 (n = 29 pairs). Sera were analyzed by microneutralization (MN) assay and a 20‐plex MAGPIX assay with rHAs from 19 influenza strains (11 subtypes) combined with serum adsorption using 8 rHAs from A(H1N1) and A(H3N2) viruses. Antibody responses were analyzed to determine the novel influenza virus exposure. RESULTS: Among persons with novel influenza virus exposures, the median fluorescence intensity (MFI) against the novel rHA from exposed influenza virus had the highest correlation with MN titers to the same viruses and could be confirmed by removal of cross‐reactivity from seasonal H1/H3 rHAs following serum adsorption. Interestingly, in persons with exposures to novel influenza viruses, age and MFIs against exposed novel HA were negatively correlated, whereas in persons without exposure to novel influenza viruses, age and MFI against novel HAs were positively correlated. CONCLUSIONS: This 20‐plex high‐throughput assay with serum adsorption will be a useful tool to detect novel influenza virus infections during influenza outbreak investigations and surveillance, especially when well‐paired serum samples are not available. John Wiley and Sons Inc. 2019-11-08 2020-03 /pmc/articles/PMC7040970/ /pubmed/31701647 http://dx.doi.org/10.1111/irv.12695 Text en © 2019 The Authors. Influenza and Other Respiratory Viruses Published by John Wiley & Sons Ltd. This is an open access article under the terms of the http://creativecommons.org/licenses/by/4.0/ License, which permits use, distribution and reproduction in any medium, provided the original work is properly cited. |
spellingShingle | Original Articles Li, Zhu‐Nan Cheng, Emily Poirot, Eugenie Weber, Kimberly M. Carney, Paul Chang, Jessie Liu, Feng Gross, F. Liaini Holiday, Crystal Fry, Alicia Stevens, James Tumpey, Terrence Levine, Min Z. Identification of novel influenza A virus exposures by an improved high‐throughput multiplex MAGPIX platform and serum adsorption |
title | Identification of novel influenza A virus exposures by an improved high‐throughput multiplex MAGPIX platform and serum adsorption |
title_full | Identification of novel influenza A virus exposures by an improved high‐throughput multiplex MAGPIX platform and serum adsorption |
title_fullStr | Identification of novel influenza A virus exposures by an improved high‐throughput multiplex MAGPIX platform and serum adsorption |
title_full_unstemmed | Identification of novel influenza A virus exposures by an improved high‐throughput multiplex MAGPIX platform and serum adsorption |
title_short | Identification of novel influenza A virus exposures by an improved high‐throughput multiplex MAGPIX platform and serum adsorption |
title_sort | identification of novel influenza a virus exposures by an improved high‐throughput multiplex magpix platform and serum adsorption |
topic | Original Articles |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7040970/ https://www.ncbi.nlm.nih.gov/pubmed/31701647 http://dx.doi.org/10.1111/irv.12695 |
work_keys_str_mv | AT lizhunan identificationofnovelinfluenzaavirusexposuresbyanimprovedhighthroughputmultiplexmagpixplatformandserumadsorption AT chengemily identificationofnovelinfluenzaavirusexposuresbyanimprovedhighthroughputmultiplexmagpixplatformandserumadsorption AT poiroteugenie identificationofnovelinfluenzaavirusexposuresbyanimprovedhighthroughputmultiplexmagpixplatformandserumadsorption AT weberkimberlym identificationofnovelinfluenzaavirusexposuresbyanimprovedhighthroughputmultiplexmagpixplatformandserumadsorption AT carneypaul identificationofnovelinfluenzaavirusexposuresbyanimprovedhighthroughputmultiplexmagpixplatformandserumadsorption AT changjessie identificationofnovelinfluenzaavirusexposuresbyanimprovedhighthroughputmultiplexmagpixplatformandserumadsorption AT liufeng identificationofnovelinfluenzaavirusexposuresbyanimprovedhighthroughputmultiplexmagpixplatformandserumadsorption AT grossfliaini identificationofnovelinfluenzaavirusexposuresbyanimprovedhighthroughputmultiplexmagpixplatformandserumadsorption AT holidaycrystal identificationofnovelinfluenzaavirusexposuresbyanimprovedhighthroughputmultiplexmagpixplatformandserumadsorption AT fryalicia identificationofnovelinfluenzaavirusexposuresbyanimprovedhighthroughputmultiplexmagpixplatformandserumadsorption AT stevensjames identificationofnovelinfluenzaavirusexposuresbyanimprovedhighthroughputmultiplexmagpixplatformandserumadsorption AT tumpeyterrence identificationofnovelinfluenzaavirusexposuresbyanimprovedhighthroughputmultiplexmagpixplatformandserumadsorption AT levineminz identificationofnovelinfluenzaavirusexposuresbyanimprovedhighthroughputmultiplexmagpixplatformandserumadsorption |