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Using the polymeric circulating tumor cell chip to capture circulating tumor cells in blood samples of patients with colorectal cancer

The current study clarified the accuracy of a circulating tumor cell (CTC) detection system to diagnose colorectal cancer using blood samples. The system uses the ‘polymeric CTC-chip,’ (CTC-chip), which is a microfluidic device that is used for CTC isolation. CTCs are considered sensitive diagnostic...

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Autores principales: Kure, Kazumasa, Hosoya, Masaki, Ueyama, Takae, Fukaya, Midori, Sugimoto, Kiichi, Tomiki, Yuichi, Ohnaga, Takashi, Sakamoto, Kazuhiro, Komiyama, Hiromitsu
Formato: Online Artículo Texto
Lenguaje:English
Publicado: D.A. Spandidos 2020
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7041365/
https://www.ncbi.nlm.nih.gov/pubmed/32194728
http://dx.doi.org/10.3892/ol.2020.11335
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author Kure, Kazumasa
Hosoya, Masaki
Ueyama, Takae
Fukaya, Midori
Sugimoto, Kiichi
Tomiki, Yuichi
Ohnaga, Takashi
Sakamoto, Kazuhiro
Komiyama, Hiromitsu
author_facet Kure, Kazumasa
Hosoya, Masaki
Ueyama, Takae
Fukaya, Midori
Sugimoto, Kiichi
Tomiki, Yuichi
Ohnaga, Takashi
Sakamoto, Kazuhiro
Komiyama, Hiromitsu
author_sort Kure, Kazumasa
collection PubMed
description The current study clarified the accuracy of a circulating tumor cell (CTC) detection system to diagnose colorectal cancer using blood samples. The system uses the ‘polymeric CTC-chip,’ (CTC-chip), which is a microfluidic device that is used for CTC isolation. CTCs are considered sensitive diagnostic biomarkers. However, their concentration in the peripheral blood is low and requires highly sensitive and specific capturing techniques. The capture efficiency of the polymeric CTC-chip was first assessed using cell suspensions of the colorectal cancer cell line HCT-116, which was reported as 90.9% in a phosphate-buffered saline suspension and 65.0% in the blood. The CTC-chip was then used to detect CTCs in blood samples obtained from 13 patients with stage II–IV colorectal cancer. On average, the CTCs/ml was lower in patients with stages II and III colorectal cancer (3.3±2.3) than in those with stage IV (7.0±6.2). In patients with stages II–IV, 92% had ≥1 CTC per ml, which was significantly higher than the positive rate (15%) detected using the carbohydrate antigen 19-9 test (CA19-9). Furthermore, CTCs were detected in all patients with stage II and III colorectal cancer, including a number of patients with negative results for the carcinoembryonic antigen (CEA) and CA19-9 tests. With the polymeric CTC-chip detection system, CTCs can be effective cancer markers, particularly for patients with stage II and III colorectal cancer who often exhibit negative conventional serum marker test results. The CTC-chip system may also facilitate the detection of cancer progression based on CTC concentration.
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spelling pubmed-70413652020-03-19 Using the polymeric circulating tumor cell chip to capture circulating tumor cells in blood samples of patients with colorectal cancer Kure, Kazumasa Hosoya, Masaki Ueyama, Takae Fukaya, Midori Sugimoto, Kiichi Tomiki, Yuichi Ohnaga, Takashi Sakamoto, Kazuhiro Komiyama, Hiromitsu Oncol Lett Articles The current study clarified the accuracy of a circulating tumor cell (CTC) detection system to diagnose colorectal cancer using blood samples. The system uses the ‘polymeric CTC-chip,’ (CTC-chip), which is a microfluidic device that is used for CTC isolation. CTCs are considered sensitive diagnostic biomarkers. However, their concentration in the peripheral blood is low and requires highly sensitive and specific capturing techniques. The capture efficiency of the polymeric CTC-chip was first assessed using cell suspensions of the colorectal cancer cell line HCT-116, which was reported as 90.9% in a phosphate-buffered saline suspension and 65.0% in the blood. The CTC-chip was then used to detect CTCs in blood samples obtained from 13 patients with stage II–IV colorectal cancer. On average, the CTCs/ml was lower in patients with stages II and III colorectal cancer (3.3±2.3) than in those with stage IV (7.0±6.2). In patients with stages II–IV, 92% had ≥1 CTC per ml, which was significantly higher than the positive rate (15%) detected using the carbohydrate antigen 19-9 test (CA19-9). Furthermore, CTCs were detected in all patients with stage II and III colorectal cancer, including a number of patients with negative results for the carcinoembryonic antigen (CEA) and CA19-9 tests. With the polymeric CTC-chip detection system, CTCs can be effective cancer markers, particularly for patients with stage II and III colorectal cancer who often exhibit negative conventional serum marker test results. The CTC-chip system may also facilitate the detection of cancer progression based on CTC concentration. D.A. Spandidos 2020-03 2020-01-23 /pmc/articles/PMC7041365/ /pubmed/32194728 http://dx.doi.org/10.3892/ol.2020.11335 Text en Copyright: © Kure et al. This is an open access article distributed under the terms of the Creative Commons Attribution-NonCommercial-NoDerivs License (https://creativecommons.org/licenses/by-nc-nd/4.0/) , which permits use and distribution in any medium, provided the original work is properly cited, the use is non-commercial and no modifications or adaptations are made.
spellingShingle Articles
Kure, Kazumasa
Hosoya, Masaki
Ueyama, Takae
Fukaya, Midori
Sugimoto, Kiichi
Tomiki, Yuichi
Ohnaga, Takashi
Sakamoto, Kazuhiro
Komiyama, Hiromitsu
Using the polymeric circulating tumor cell chip to capture circulating tumor cells in blood samples of patients with colorectal cancer
title Using the polymeric circulating tumor cell chip to capture circulating tumor cells in blood samples of patients with colorectal cancer
title_full Using the polymeric circulating tumor cell chip to capture circulating tumor cells in blood samples of patients with colorectal cancer
title_fullStr Using the polymeric circulating tumor cell chip to capture circulating tumor cells in blood samples of patients with colorectal cancer
title_full_unstemmed Using the polymeric circulating tumor cell chip to capture circulating tumor cells in blood samples of patients with colorectal cancer
title_short Using the polymeric circulating tumor cell chip to capture circulating tumor cells in blood samples of patients with colorectal cancer
title_sort using the polymeric circulating tumor cell chip to capture circulating tumor cells in blood samples of patients with colorectal cancer
topic Articles
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7041365/
https://www.ncbi.nlm.nih.gov/pubmed/32194728
http://dx.doi.org/10.3892/ol.2020.11335
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