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Rho-associated protein kinase inhibitor induced morphological changes in type VI collagen in the human trabecular meshwork

AIMS: To investigate morphological changes in type VI collagen in the human trabecular meshwork associated with the rho kinase inhibitor ripasudil. METHODS: This cross-sectional study evaluated the effects of ripasudil eye drop administration (RA) or no ripasudil eye drop administration (NRA) in pat...

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Autores principales: Okamoto, Mizu, Nagahara, Miyuki, Tajiri, Takuma, Nakamura, Naoya, Fukunishi, Nahoko, Nagahara, Kentaro
Formato: Online Artículo Texto
Lenguaje:English
Publicado: BMJ Publishing Group 2020
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Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7041502/
https://www.ncbi.nlm.nih.gov/pubmed/31201167
http://dx.doi.org/10.1136/bjophthalmol-2018-312991
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author Okamoto, Mizu
Nagahara, Miyuki
Tajiri, Takuma
Nakamura, Naoya
Fukunishi, Nahoko
Nagahara, Kentaro
author_facet Okamoto, Mizu
Nagahara, Miyuki
Tajiri, Takuma
Nakamura, Naoya
Fukunishi, Nahoko
Nagahara, Kentaro
author_sort Okamoto, Mizu
collection PubMed
description AIMS: To investigate morphological changes in type VI collagen in the human trabecular meshwork associated with the rho kinase inhibitor ripasudil. METHODS: This cross-sectional study evaluated the effects of ripasudil eye drop administration (RA) or no ripasudil eye drop administration (NRA) in patients with primary open-angle glaucoma (POAG; age range 60–80 years) who underwent conventional outflow reconstruction between December 2015 and September 2016 at Tokai University Hachioji Hospital. The juxtacanalicular tissue was removed and imaged using transmission electron microscopy. Type VI collagen comprises cross-banded aggregates with transverse bands 30 nm apart repeating every 105 nm. The transverse bands are called the outer rod-like region (ORR) and the intervals are called the inner rod-like region (IRR). The waveform intensity in the type VI collagen was analysed in electron micrographs using Fourier transformation to detect the IRR and ORR borders. RESULTS: Ten eyes of 10 patients were included (n=5/group). The baseline characteristics did not differ significantly between groups. ORR width was significantly smaller in the RA group (37.85±3.43 nm) than in the NRA group (50.62±5.23 nm, p<0.05), whereas IRR width was significantly greater in the RA group (70.68±10.84 nm) than in the NRA group (58.19±5.34 nm, p<0.05). Morphological changes in the type VI collagen total width tended to correlate with the duration of ripasudil administration (r=0.9, p=0.08). CONCLUSIONS: Ripasudil administration in patients with POAG induced morphological changes in type VI collagen. Patients with POAG administered RA had a significantly smaller ORR width and a significantly greater IRR width than patients with POAG not administered RA.
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spelling pubmed-70415022020-03-03 Rho-associated protein kinase inhibitor induced morphological changes in type VI collagen in the human trabecular meshwork Okamoto, Mizu Nagahara, Miyuki Tajiri, Takuma Nakamura, Naoya Fukunishi, Nahoko Nagahara, Kentaro Br J Ophthalmol Clinical Science AIMS: To investigate morphological changes in type VI collagen in the human trabecular meshwork associated with the rho kinase inhibitor ripasudil. METHODS: This cross-sectional study evaluated the effects of ripasudil eye drop administration (RA) or no ripasudil eye drop administration (NRA) in patients with primary open-angle glaucoma (POAG; age range 60–80 years) who underwent conventional outflow reconstruction between December 2015 and September 2016 at Tokai University Hachioji Hospital. The juxtacanalicular tissue was removed and imaged using transmission electron microscopy. Type VI collagen comprises cross-banded aggregates with transverse bands 30 nm apart repeating every 105 nm. The transverse bands are called the outer rod-like region (ORR) and the intervals are called the inner rod-like region (IRR). The waveform intensity in the type VI collagen was analysed in electron micrographs using Fourier transformation to detect the IRR and ORR borders. RESULTS: Ten eyes of 10 patients were included (n=5/group). The baseline characteristics did not differ significantly between groups. ORR width was significantly smaller in the RA group (37.85±3.43 nm) than in the NRA group (50.62±5.23 nm, p<0.05), whereas IRR width was significantly greater in the RA group (70.68±10.84 nm) than in the NRA group (58.19±5.34 nm, p<0.05). Morphological changes in the type VI collagen total width tended to correlate with the duration of ripasudil administration (r=0.9, p=0.08). CONCLUSIONS: Ripasudil administration in patients with POAG induced morphological changes in type VI collagen. Patients with POAG administered RA had a significantly smaller ORR width and a significantly greater IRR width than patients with POAG not administered RA. BMJ Publishing Group 2020-03 2019-06-14 /pmc/articles/PMC7041502/ /pubmed/31201167 http://dx.doi.org/10.1136/bjophthalmol-2018-312991 Text en © Author(s) (or their employer(s)) 2020. Re-use permitted under CC BY-NC. No commercial re-use. See rights and permissions. Published by BMJ. http://creativecommons.org/licenses/by-nc/4.0/This is an open access article distributed in accordance with the Creative Commons Attribution Non Commercial (CC BY-NC 4.0) license, which permits others to distribute, remix, adapt, build upon this work non-commercially, and license their derivative works on different terms, provided the original work is properly cited, appropriate credit is given, any changes made indicated, and the use is non-commercial. See: http://creativecommons.org/licenses/by-nc/4.0/.
spellingShingle Clinical Science
Okamoto, Mizu
Nagahara, Miyuki
Tajiri, Takuma
Nakamura, Naoya
Fukunishi, Nahoko
Nagahara, Kentaro
Rho-associated protein kinase inhibitor induced morphological changes in type VI collagen in the human trabecular meshwork
title Rho-associated protein kinase inhibitor induced morphological changes in type VI collagen in the human trabecular meshwork
title_full Rho-associated protein kinase inhibitor induced morphological changes in type VI collagen in the human trabecular meshwork
title_fullStr Rho-associated protein kinase inhibitor induced morphological changes in type VI collagen in the human trabecular meshwork
title_full_unstemmed Rho-associated protein kinase inhibitor induced morphological changes in type VI collagen in the human trabecular meshwork
title_short Rho-associated protein kinase inhibitor induced morphological changes in type VI collagen in the human trabecular meshwork
title_sort rho-associated protein kinase inhibitor induced morphological changes in type vi collagen in the human trabecular meshwork
topic Clinical Science
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7041502/
https://www.ncbi.nlm.nih.gov/pubmed/31201167
http://dx.doi.org/10.1136/bjophthalmol-2018-312991
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