Cargando…
Circular RNA expression in isoproterenol hydrochloride-induced cardiac hypertrophy
Circular RNA (circRNA) is a novel class of noncoding RNAs, and the roles of circRNAs in the development of cardiac hypertrophy remain to be explored. Here, we investigate the potential roles of circRNAs in cardiac hypertrophy. By circRNA sequencing in left ventricular specimens collected from 8-week...
Autores principales: | , , , , , , , , , , |
---|---|
Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Impact Journals
2020
|
Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7041747/ https://www.ncbi.nlm.nih.gov/pubmed/32023551 http://dx.doi.org/10.18632/aging.102761 |
_version_ | 1783501201170497536 |
---|---|
author | Yang, Ming-Hui Wang, Hao Han, Sheng-Na Jia, Xin Zhang, Si Dai, Fei-Fei Zhou, Meng-Jiao Yin, Zhongnan Wang, Tian-Qi Zang, Ming-Xi Xue, Li-Xiang |
author_facet | Yang, Ming-Hui Wang, Hao Han, Sheng-Na Jia, Xin Zhang, Si Dai, Fei-Fei Zhou, Meng-Jiao Yin, Zhongnan Wang, Tian-Qi Zang, Ming-Xi Xue, Li-Xiang |
author_sort | Yang, Ming-Hui |
collection | PubMed |
description | Circular RNA (circRNA) is a novel class of noncoding RNAs, and the roles of circRNAs in the development of cardiac hypertrophy remain to be explored. Here, we investigate the potential roles of circRNAs in cardiac hypertrophy. By circRNA sequencing in left ventricular specimens collected from 8-week-old mice with isoproterenol hydrochloride-induced cardiac hypertrophy, we found 401 out of 3323 total circRNAs were dysregulated in the hypertrophic hearts compared with the controls. Of these, 303 circRNAs were upregulated and 98 were downregulated. Moreover, the GO and KEGG analyses revealed that the majority of parental gene of differentially expressed circRNAs were not only related to biological process such as metabolic process and response to stimulus, but also related to pathway such as circulatory system and cardiovascular diseases. On the other hand, total 1974 miRNAs were predicted to binding to these differentially expressed circRNAs, and the possible target mRNAs of those miRNAs were also predicted and analyzed in terms of functional annotation. Finally, we identified that ANF and miR-23a are downstream targets of circRNA wwp1, suggesting that circRNA wwp1 exerts inhibitory roles of cardiac hypertrophy via down-regulation of ANF and miR-23a, which underlying the potential mechanisms whereby circRNA regulates cardiac hypertrophy. |
format | Online Article Text |
id | pubmed-7041747 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2020 |
publisher | Impact Journals |
record_format | MEDLINE/PubMed |
spelling | pubmed-70417472020-03-04 Circular RNA expression in isoproterenol hydrochloride-induced cardiac hypertrophy Yang, Ming-Hui Wang, Hao Han, Sheng-Na Jia, Xin Zhang, Si Dai, Fei-Fei Zhou, Meng-Jiao Yin, Zhongnan Wang, Tian-Qi Zang, Ming-Xi Xue, Li-Xiang Aging (Albany NY) Research Paper Circular RNA (circRNA) is a novel class of noncoding RNAs, and the roles of circRNAs in the development of cardiac hypertrophy remain to be explored. Here, we investigate the potential roles of circRNAs in cardiac hypertrophy. By circRNA sequencing in left ventricular specimens collected from 8-week-old mice with isoproterenol hydrochloride-induced cardiac hypertrophy, we found 401 out of 3323 total circRNAs were dysregulated in the hypertrophic hearts compared with the controls. Of these, 303 circRNAs were upregulated and 98 were downregulated. Moreover, the GO and KEGG analyses revealed that the majority of parental gene of differentially expressed circRNAs were not only related to biological process such as metabolic process and response to stimulus, but also related to pathway such as circulatory system and cardiovascular diseases. On the other hand, total 1974 miRNAs were predicted to binding to these differentially expressed circRNAs, and the possible target mRNAs of those miRNAs were also predicted and analyzed in terms of functional annotation. Finally, we identified that ANF and miR-23a are downstream targets of circRNA wwp1, suggesting that circRNA wwp1 exerts inhibitory roles of cardiac hypertrophy via down-regulation of ANF and miR-23a, which underlying the potential mechanisms whereby circRNA regulates cardiac hypertrophy. Impact Journals 2020-02-05 /pmc/articles/PMC7041747/ /pubmed/32023551 http://dx.doi.org/10.18632/aging.102761 Text en Copyright © 2020 Yang et al. http://creativecommons.org/licenses/by/3.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY 3.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited. |
spellingShingle | Research Paper Yang, Ming-Hui Wang, Hao Han, Sheng-Na Jia, Xin Zhang, Si Dai, Fei-Fei Zhou, Meng-Jiao Yin, Zhongnan Wang, Tian-Qi Zang, Ming-Xi Xue, Li-Xiang Circular RNA expression in isoproterenol hydrochloride-induced cardiac hypertrophy |
title | Circular RNA expression in isoproterenol hydrochloride-induced cardiac hypertrophy |
title_full | Circular RNA expression in isoproterenol hydrochloride-induced cardiac hypertrophy |
title_fullStr | Circular RNA expression in isoproterenol hydrochloride-induced cardiac hypertrophy |
title_full_unstemmed | Circular RNA expression in isoproterenol hydrochloride-induced cardiac hypertrophy |
title_short | Circular RNA expression in isoproterenol hydrochloride-induced cardiac hypertrophy |
title_sort | circular rna expression in isoproterenol hydrochloride-induced cardiac hypertrophy |
topic | Research Paper |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7041747/ https://www.ncbi.nlm.nih.gov/pubmed/32023551 http://dx.doi.org/10.18632/aging.102761 |
work_keys_str_mv | AT yangminghui circularrnaexpressioninisoproterenolhydrochlorideinducedcardiachypertrophy AT wanghao circularrnaexpressioninisoproterenolhydrochlorideinducedcardiachypertrophy AT hanshengna circularrnaexpressioninisoproterenolhydrochlorideinducedcardiachypertrophy AT jiaxin circularrnaexpressioninisoproterenolhydrochlorideinducedcardiachypertrophy AT zhangsi circularrnaexpressioninisoproterenolhydrochlorideinducedcardiachypertrophy AT daifeifei circularrnaexpressioninisoproterenolhydrochlorideinducedcardiachypertrophy AT zhoumengjiao circularrnaexpressioninisoproterenolhydrochlorideinducedcardiachypertrophy AT yinzhongnan circularrnaexpressioninisoproterenolhydrochlorideinducedcardiachypertrophy AT wangtianqi circularrnaexpressioninisoproterenolhydrochlorideinducedcardiachypertrophy AT zangmingxi circularrnaexpressioninisoproterenolhydrochlorideinducedcardiachypertrophy AT xuelixiang circularrnaexpressioninisoproterenolhydrochlorideinducedcardiachypertrophy |