FTA-Sodium hydroxide-based polymerase chain reaction (PCR): An efficient and cheaper option for Theileria parva detection in dairy cattle in Mbarara, Uganda

East Coast fever is caused by Theileria parva, and poses serious concerns for dairy farmers owing to massive economic losses. In the current study, we compared three methods (DNA extraction kits, FTA-NaOH and FTA-TENT) of DNA extraction to identify the most economical and reliable method. A survey f...

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Autores principales: UCHIDA, Leo, BYARUHANGA, Joseph, OKAMURA, Ikuo, MIYAMA, Takeshi, MURAMATSU, Yasukazu, VUDRIKO, Patrick, MAKITA, Kohei
Formato: Online Artículo Texto
Lenguaje:English
Publicado: The Japanese Society of Veterinary Science 2019
Materias:
Parasitology
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7041997/
https://www.ncbi.nlm.nih.gov/pubmed/31875599
http://dx.doi.org/10.1292/jvms.19-0521
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author UCHIDA, Leo
BYARUHANGA, Joseph
OKAMURA, Ikuo
MIYAMA, Takeshi
MURAMATSU, Yasukazu
VUDRIKO, Patrick
MAKITA, Kohei
author_facet UCHIDA, Leo
BYARUHANGA, Joseph
OKAMURA, Ikuo
MIYAMA, Takeshi
MURAMATSU, Yasukazu
VUDRIKO, Patrick
MAKITA, Kohei
author_sort UCHIDA, Leo
collection PubMed
description East Coast fever is caused by Theileria parva, and poses serious concerns for dairy farmers owing to massive economic losses. In the current study, we compared three methods (DNA extraction kits, FTA-NaOH and FTA-TENT) of DNA extraction to identify the most economical and reliable method. A survey for T. parva prevalence was conducted in dairy cattle in Mbarara, Uganda. Cytochrome C oxidase subunit I (COI) and T. parva-p104 genes were amplified to compare the methods. FTA-NaOH-based polymerase chain reaction (PCR) yielded the best detection rate for both COI gene and p104 gene. Prevalence of T. parva was 45.0% and 83.3% at animal and farm-level, respectively. FTA-NaOH based-PCR is simple, highly sensitive and cost-effective tool for T. parva diagnosis in resource constrained settings.
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spelling pubmed-70419972020-03-02 FTA-Sodium hydroxide-based polymerase chain reaction (PCR): An efficient and cheaper option for Theileria parva detection in dairy cattle in Mbarara, Uganda UCHIDA, Leo BYARUHANGA, Joseph OKAMURA, Ikuo MIYAMA, Takeshi MURAMATSU, Yasukazu VUDRIKO, Patrick MAKITA, Kohei J Vet Med Sci Parasitology East Coast fever is caused by Theileria parva, and poses serious concerns for dairy farmers owing to massive economic losses. In the current study, we compared three methods (DNA extraction kits, FTA-NaOH and FTA-TENT) of DNA extraction to identify the most economical and reliable method. A survey for T. parva prevalence was conducted in dairy cattle in Mbarara, Uganda. Cytochrome C oxidase subunit I (COI) and T. parva-p104 genes were amplified to compare the methods. FTA-NaOH-based polymerase chain reaction (PCR) yielded the best detection rate for both COI gene and p104 gene. Prevalence of T. parva was 45.0% and 83.3% at animal and farm-level, respectively. FTA-NaOH based-PCR is simple, highly sensitive and cost-effective tool for T. parva diagnosis in resource constrained settings. The Japanese Society of Veterinary Science 2019-12-25 2020-02 /pmc/articles/PMC7041997/ /pubmed/31875599 http://dx.doi.org/10.1292/jvms.19-0521 Text en ©2020 The Japanese Society of Veterinary Science This is an open-access article distributed under the terms of the Creative Commons Attribution Non-Commercial No Derivatives (by-nc-nd) License. (CC-BY-NC-ND 4.0: https://creativecommons.org/licenses/by-nc-nd/4.0/)
spellingShingle Parasitology
UCHIDA, Leo
BYARUHANGA, Joseph
OKAMURA, Ikuo
MIYAMA, Takeshi
MURAMATSU, Yasukazu
VUDRIKO, Patrick
MAKITA, Kohei
FTA-Sodium hydroxide-based polymerase chain reaction (PCR): An efficient and cheaper option for Theileria parva detection in dairy cattle in Mbarara, Uganda
title FTA-Sodium hydroxide-based polymerase chain reaction (PCR): An efficient and cheaper option for Theileria parva detection in dairy cattle in Mbarara, Uganda
title_full FTA-Sodium hydroxide-based polymerase chain reaction (PCR): An efficient and cheaper option for Theileria parva detection in dairy cattle in Mbarara, Uganda
title_fullStr FTA-Sodium hydroxide-based polymerase chain reaction (PCR): An efficient and cheaper option for Theileria parva detection in dairy cattle in Mbarara, Uganda
title_full_unstemmed FTA-Sodium hydroxide-based polymerase chain reaction (PCR): An efficient and cheaper option for Theileria parva detection in dairy cattle in Mbarara, Uganda
title_short FTA-Sodium hydroxide-based polymerase chain reaction (PCR): An efficient and cheaper option for Theileria parva detection in dairy cattle in Mbarara, Uganda
title_sort fta-sodium hydroxide-based polymerase chain reaction (pcr): an efficient and cheaper option for theileria parva detection in dairy cattle in mbarara, uganda
topic Parasitology
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7041997/
https://www.ncbi.nlm.nih.gov/pubmed/31875599
http://dx.doi.org/10.1292/jvms.19-0521
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