Cargando…

Comparative Proteomics of Extended-Spectrum Cephalosporin-Resistant Neisseria gonorrhoeae Isolates Demonstrates Altered Protein Synthesis, Metabolism, Substance Transport, and Membrane Permeability

Neisseria gonorrhoeae isolates exhibit resistance to extended-spectrum cephalosporins (ESCs), the last remaining option for first-line empirical monotherapy. Here, we investigated the proteomic profiles of N. gonorrhoeae clinical isolates with ESC-resistance to support exploration of the antimicrobi...

Descripción completa

Detalles Bibliográficos
Autores principales: Diao, Nannan, Yan, Guoquan, Yang, Yang, Dong, Yuan, Wang, Ying, Gu, Weiming
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Frontiers Media S.A. 2020
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7042406/
https://www.ncbi.nlm.nih.gov/pubmed/32140142
http://dx.doi.org/10.3389/fmicb.2020.00169
_version_ 1783501304800215040
author Diao, Nannan
Yan, Guoquan
Yang, Yang
Dong, Yuan
Wang, Ying
Gu, Weiming
author_facet Diao, Nannan
Yan, Guoquan
Yang, Yang
Dong, Yuan
Wang, Ying
Gu, Weiming
author_sort Diao, Nannan
collection PubMed
description Neisseria gonorrhoeae isolates exhibit resistance to extended-spectrum cephalosporins (ESCs), the last remaining option for first-line empirical monotherapy. Here, we investigated the proteomic profiles of N. gonorrhoeae clinical isolates with ESC-resistance to support exploration of the antimicrobial resistance mechanisms for N. gonorrhoeae. We used comparative iTRAQ quantitative proteomics to investigate differential protein expression of three ESC-resistant N. gonorrhoeae clinical isolates using N. gonorrhoeae ATCC49226 as a reference strain. The expression of 40 proteins was downregulated and expression of 56 proteins was upregulated in all three ESC-resistant N. gonorrhoeae isolates. Proteins with predicted function of translation, ribosomal structure and biogenesis, as well as components of the Type IV secretory systems, were significantly upregulated. Two differentially expressed proteins of ABC transporters were also reported by other teams in proteomics studies of N. gonorrhoeae isolates under antimicrobial stress conditions. Differentially expressed proteins are involved in energy production and metabolism of carbohydrates and amino acids. Our results indicated that amino acid and carbohydrate metabolism, cell membrane structure, interbacterial DNA transfer, and ribosome components might be involved in mediating ESC-resistance in N. gonorrhoeae. These findings facilitate a better understanding of the mechanisms of ESC-resistance in N. gonorrhoeae and provide useful information for identifying novel targets in the development of antimicrobials against N. gonorrhoeae.
format Online
Article
Text
id pubmed-7042406
institution National Center for Biotechnology Information
language English
publishDate 2020
publisher Frontiers Media S.A.
record_format MEDLINE/PubMed
spelling pubmed-70424062020-03-05 Comparative Proteomics of Extended-Spectrum Cephalosporin-Resistant Neisseria gonorrhoeae Isolates Demonstrates Altered Protein Synthesis, Metabolism, Substance Transport, and Membrane Permeability Diao, Nannan Yan, Guoquan Yang, Yang Dong, Yuan Wang, Ying Gu, Weiming Front Microbiol Microbiology Neisseria gonorrhoeae isolates exhibit resistance to extended-spectrum cephalosporins (ESCs), the last remaining option for first-line empirical monotherapy. Here, we investigated the proteomic profiles of N. gonorrhoeae clinical isolates with ESC-resistance to support exploration of the antimicrobial resistance mechanisms for N. gonorrhoeae. We used comparative iTRAQ quantitative proteomics to investigate differential protein expression of three ESC-resistant N. gonorrhoeae clinical isolates using N. gonorrhoeae ATCC49226 as a reference strain. The expression of 40 proteins was downregulated and expression of 56 proteins was upregulated in all three ESC-resistant N. gonorrhoeae isolates. Proteins with predicted function of translation, ribosomal structure and biogenesis, as well as components of the Type IV secretory systems, were significantly upregulated. Two differentially expressed proteins of ABC transporters were also reported by other teams in proteomics studies of N. gonorrhoeae isolates under antimicrobial stress conditions. Differentially expressed proteins are involved in energy production and metabolism of carbohydrates and amino acids. Our results indicated that amino acid and carbohydrate metabolism, cell membrane structure, interbacterial DNA transfer, and ribosome components might be involved in mediating ESC-resistance in N. gonorrhoeae. These findings facilitate a better understanding of the mechanisms of ESC-resistance in N. gonorrhoeae and provide useful information for identifying novel targets in the development of antimicrobials against N. gonorrhoeae. Frontiers Media S.A. 2020-02-19 /pmc/articles/PMC7042406/ /pubmed/32140142 http://dx.doi.org/10.3389/fmicb.2020.00169 Text en Copyright © 2020 Diao, Yan, Yang, Dong, Wang and Gu. http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
spellingShingle Microbiology
Diao, Nannan
Yan, Guoquan
Yang, Yang
Dong, Yuan
Wang, Ying
Gu, Weiming
Comparative Proteomics of Extended-Spectrum Cephalosporin-Resistant Neisseria gonorrhoeae Isolates Demonstrates Altered Protein Synthesis, Metabolism, Substance Transport, and Membrane Permeability
title Comparative Proteomics of Extended-Spectrum Cephalosporin-Resistant Neisseria gonorrhoeae Isolates Demonstrates Altered Protein Synthesis, Metabolism, Substance Transport, and Membrane Permeability
title_full Comparative Proteomics of Extended-Spectrum Cephalosporin-Resistant Neisseria gonorrhoeae Isolates Demonstrates Altered Protein Synthesis, Metabolism, Substance Transport, and Membrane Permeability
title_fullStr Comparative Proteomics of Extended-Spectrum Cephalosporin-Resistant Neisseria gonorrhoeae Isolates Demonstrates Altered Protein Synthesis, Metabolism, Substance Transport, and Membrane Permeability
title_full_unstemmed Comparative Proteomics of Extended-Spectrum Cephalosporin-Resistant Neisseria gonorrhoeae Isolates Demonstrates Altered Protein Synthesis, Metabolism, Substance Transport, and Membrane Permeability
title_short Comparative Proteomics of Extended-Spectrum Cephalosporin-Resistant Neisseria gonorrhoeae Isolates Demonstrates Altered Protein Synthesis, Metabolism, Substance Transport, and Membrane Permeability
title_sort comparative proteomics of extended-spectrum cephalosporin-resistant neisseria gonorrhoeae isolates demonstrates altered protein synthesis, metabolism, substance transport, and membrane permeability
topic Microbiology
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7042406/
https://www.ncbi.nlm.nih.gov/pubmed/32140142
http://dx.doi.org/10.3389/fmicb.2020.00169
work_keys_str_mv AT diaonannan comparativeproteomicsofextendedspectrumcephalosporinresistantneisseriagonorrhoeaeisolatesdemonstratesalteredproteinsynthesismetabolismsubstancetransportandmembranepermeability
AT yanguoquan comparativeproteomicsofextendedspectrumcephalosporinresistantneisseriagonorrhoeaeisolatesdemonstratesalteredproteinsynthesismetabolismsubstancetransportandmembranepermeability
AT yangyang comparativeproteomicsofextendedspectrumcephalosporinresistantneisseriagonorrhoeaeisolatesdemonstratesalteredproteinsynthesismetabolismsubstancetransportandmembranepermeability
AT dongyuan comparativeproteomicsofextendedspectrumcephalosporinresistantneisseriagonorrhoeaeisolatesdemonstratesalteredproteinsynthesismetabolismsubstancetransportandmembranepermeability
AT wangying comparativeproteomicsofextendedspectrumcephalosporinresistantneisseriagonorrhoeaeisolatesdemonstratesalteredproteinsynthesismetabolismsubstancetransportandmembranepermeability
AT guweiming comparativeproteomicsofextendedspectrumcephalosporinresistantneisseriagonorrhoeaeisolatesdemonstratesalteredproteinsynthesismetabolismsubstancetransportandmembranepermeability