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Cultivation of Bacteria From Aplysina aerophoba: Effects of Oxygen and Nutrient Gradients
Sponge-associated bacteria possess biotechnologically interesting properties but as yet have largely evaded cultivation. Thus, “omics”-based information on the ecology and functional potential of sponge symbionts is awaiting its integration into the design of innovative cultivation approaches. To cu...
Autores principales: | , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
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Frontiers Media S.A.
2020
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Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7042410/ https://www.ncbi.nlm.nih.gov/pubmed/32140143 http://dx.doi.org/10.3389/fmicb.2020.00175 |
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author | Gutleben, Johanna Loureiro, Catarina Ramírez Romero, Laura Adriana Shetty, Sudarshan Wijffels, René H. Smidt, Hauke Sipkema, Detmer |
author_facet | Gutleben, Johanna Loureiro, Catarina Ramírez Romero, Laura Adriana Shetty, Sudarshan Wijffels, René H. Smidt, Hauke Sipkema, Detmer |
author_sort | Gutleben, Johanna |
collection | PubMed |
description | Sponge-associated bacteria possess biotechnologically interesting properties but as yet have largely evaded cultivation. Thus, “omics”-based information on the ecology and functional potential of sponge symbionts is awaiting its integration into the design of innovative cultivation approaches. To cultivate bacteria derived from the marine sponge Aplysina aerophoba, nine novel media formulations were created based on the predicted genomic potential of the prevalent sponge symbiont lineage Poribacteria. In addition, to maintain potential microbial metabolic interactions in vitro, a Liquid-Solid cultivation approach and a Winogradsky-column approach were applied. The vast majority of microorganisms in the inoculum appeared viable after cryopreservation of sponge specimen as determined by selective propidium monoazide DNA modification of membrane-compromised cells, however, only 2% of the initial prokaryotic diversity could be recovered through cultivation. In total, 256 OTUs encompassing seven prokaryotic phyla were cultivated. The diversity of the cultivated community was influenced by the addition of the antibiotic aeroplysinin-1 as well as by medium dilution, rather than carbon source. Furthermore, the Winogradsky-column approach reproducibly enriched distinct communities at different column depths, amongst which were numerous Clostridia and OTUs that could not be assigned to a known phylum. While some bacterial taxa such as Pseudovibrio and Ruegeria were recovered from nearly all applied cultivation conditions, others such as Bacteroidetes were specific to certain medium types. Predominant sponge-associated prokaryotic taxa remained uncultured, nonetheless, alternative cultivation approaches applied here enriched for previously uncultivated microbes. |
format | Online Article Text |
id | pubmed-7042410 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2020 |
publisher | Frontiers Media S.A. |
record_format | MEDLINE/PubMed |
spelling | pubmed-70424102020-03-05 Cultivation of Bacteria From Aplysina aerophoba: Effects of Oxygen and Nutrient Gradients Gutleben, Johanna Loureiro, Catarina Ramírez Romero, Laura Adriana Shetty, Sudarshan Wijffels, René H. Smidt, Hauke Sipkema, Detmer Front Microbiol Microbiology Sponge-associated bacteria possess biotechnologically interesting properties but as yet have largely evaded cultivation. Thus, “omics”-based information on the ecology and functional potential of sponge symbionts is awaiting its integration into the design of innovative cultivation approaches. To cultivate bacteria derived from the marine sponge Aplysina aerophoba, nine novel media formulations were created based on the predicted genomic potential of the prevalent sponge symbiont lineage Poribacteria. In addition, to maintain potential microbial metabolic interactions in vitro, a Liquid-Solid cultivation approach and a Winogradsky-column approach were applied. The vast majority of microorganisms in the inoculum appeared viable after cryopreservation of sponge specimen as determined by selective propidium monoazide DNA modification of membrane-compromised cells, however, only 2% of the initial prokaryotic diversity could be recovered through cultivation. In total, 256 OTUs encompassing seven prokaryotic phyla were cultivated. The diversity of the cultivated community was influenced by the addition of the antibiotic aeroplysinin-1 as well as by medium dilution, rather than carbon source. Furthermore, the Winogradsky-column approach reproducibly enriched distinct communities at different column depths, amongst which were numerous Clostridia and OTUs that could not be assigned to a known phylum. While some bacterial taxa such as Pseudovibrio and Ruegeria were recovered from nearly all applied cultivation conditions, others such as Bacteroidetes were specific to certain medium types. Predominant sponge-associated prokaryotic taxa remained uncultured, nonetheless, alternative cultivation approaches applied here enriched for previously uncultivated microbes. Frontiers Media S.A. 2020-02-19 /pmc/articles/PMC7042410/ /pubmed/32140143 http://dx.doi.org/10.3389/fmicb.2020.00175 Text en Copyright © 2020 Gutleben, Loureiro, Ramírez Romero, Shetty, Wijffels, Smidt and Sipkema. http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms. |
spellingShingle | Microbiology Gutleben, Johanna Loureiro, Catarina Ramírez Romero, Laura Adriana Shetty, Sudarshan Wijffels, René H. Smidt, Hauke Sipkema, Detmer Cultivation of Bacteria From Aplysina aerophoba: Effects of Oxygen and Nutrient Gradients |
title | Cultivation of Bacteria From Aplysina aerophoba: Effects of Oxygen and Nutrient Gradients |
title_full | Cultivation of Bacteria From Aplysina aerophoba: Effects of Oxygen and Nutrient Gradients |
title_fullStr | Cultivation of Bacteria From Aplysina aerophoba: Effects of Oxygen and Nutrient Gradients |
title_full_unstemmed | Cultivation of Bacteria From Aplysina aerophoba: Effects of Oxygen and Nutrient Gradients |
title_short | Cultivation of Bacteria From Aplysina aerophoba: Effects of Oxygen and Nutrient Gradients |
title_sort | cultivation of bacteria from aplysina aerophoba: effects of oxygen and nutrient gradients |
topic | Microbiology |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7042410/ https://www.ncbi.nlm.nih.gov/pubmed/32140143 http://dx.doi.org/10.3389/fmicb.2020.00175 |
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