Biological control of yeast contamination of industrial foods by propolis

Bee glue (Propolis, PR), mixture of beeswax and resin is collected from honeybee (Apis mellifera) of different plant parts. The antimicrobial potential of PR against food borne yeast was reported. The experiment was designed to examine the way of antimicrobial impact of PR on food borne yeasts (Cryptococcus laurentii and Candida famata) and its usage use as biological strategy for the preservation of soft foods against microbial spoilage. The study also highlights, the ability of ethanol and water- PR extracts, discouraged growth of tested yeast. Antifungal properties were also determined using electron microscope while biochemical analysis was determined using free and proteinic amino acid technique and oxidative enzymes were determined using HPLC analysis. Antioxidant enzymes were determined using ELISA assay. The highest effect was recorded on C. laurentii however, the lowest effect shows on C. famata. The electron microscopic studies clearly disclosed the effect of water PR distillate on the external shape and internal organs of some tested yeast e.g. C. laurentii and C. famata. The result indicated some differences on concentrations of bio-chemical analyses for these tested yeasts treated with 70% water- PR extracts of different food materials. Moreover, biochemical analysis results also reported that the treated yeast indicated natural preservative to food products and considered as best alternative to the (chemical) preservatives currently employed.