Nitrogen isotope ratios trace high-pH conditions in a terrestrial Mars analog site

High-pH alkaline lakes are among the most productive ecosystems on Earth and prime targets in the search for life on Mars; however, a robust proxy for such settings does not yet exist. Nitrogen isotope fractionation resulting from NH(3) volatilization at high pH has the potential to fill this gap. To validate this idea, we analyzed samples from the Nördlinger Ries, a Miocene impact crater lake that displayed pH values up to 9.8 as inferred from mineralogy and aqueous modeling. Our data show a peak in δ(15)N of +17‰ in the most alkaline facies, followed by a gradual decline to around +5‰, concurrent with the proposed decline in pH, highlighting the utility of nitrogen isotopes as a proxy for high-pH conditions. In combination with independent mineralogical indicators for high alkalinity, nitrogen isotopes can provide much-needed quantitative constraints on ancient atmospheric Pco(2) (partial pressure of CO(2)) and thus climatic controls on early Earth and Mars.