Cargando…
The absence of SOX2 in the anterior foregut alters the esophagus into trachea and bronchi in both epithelial and mesenchymal components
In the anterior foregut (AFG) of mouse embryos, the transcription factor SOX2 is expressed in the epithelia of the esophagus and proximal branches of respiratory organs comprising the trachea and bronchi, whereas NKX2.1 is expressed only in the epithelia of respiratory organs. Previous studies using...
Autores principales: | , , , , , , , |
---|---|
Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
The Company of Biologists Ltd
2020
|
Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7044460/ https://www.ncbi.nlm.nih.gov/pubmed/31988094 http://dx.doi.org/10.1242/bio.048728 |
_version_ | 1783501574717308928 |
---|---|
author | Teramoto, Machiko Sugawara, Ryo Minegishi, Katsura Uchikawa, Masanori Takemoto, Tatsuya Kuroiwa, Atsushi Ishii, Yasuo Kondoh, Hisato |
author_facet | Teramoto, Machiko Sugawara, Ryo Minegishi, Katsura Uchikawa, Masanori Takemoto, Tatsuya Kuroiwa, Atsushi Ishii, Yasuo Kondoh, Hisato |
author_sort | Teramoto, Machiko |
collection | PubMed |
description | In the anterior foregut (AFG) of mouse embryos, the transcription factor SOX2 is expressed in the epithelia of the esophagus and proximal branches of respiratory organs comprising the trachea and bronchi, whereas NKX2.1 is expressed only in the epithelia of respiratory organs. Previous studies using hypomorphic Sox2 alleles have indicated that reduced SOX2 expression causes the esophageal epithelium to display some respiratory organ characteristics. In the present study, we produced mouse embryos with AFG-specific SOX2 deficiency. In the absence of SOX2 expression, a single NKX2.1-expressing epithelial tube connected the pharynx and the stomach, and a pair of bronchi developed in the middle of the tube. Expression patterns of NKX2.1 and SOX9 revealed that the anterior and posterior halves of SOX2-deficient AFG epithelial tubes assumed the characteristics of the trachea and bronchus, respectively. In addition, we found that mesenchymal tissues surrounding the SOX2-deficient NKX2.1-expressing epithelial tube changed to those surrounding the trachea and bronchi in the anterior and posterior halves, as indicated by the arrangement of smooth muscle cells and SOX9-expressing cells and by the expression of Wnt4 (esophagus specific), Tbx4 (respiratory organ specific), and Hoxb6 (distal bronchus specific). The impact of mesenchyme-derived signaling on the early stage of AFG epithelial specification has been indicated. Our study demonstrated an opposite trend where epithelial tissue specification causes concordant changes in mesenchymal tissues, indicating a reciprocity of epithelial-mesenchymal interactions. |
format | Online Article Text |
id | pubmed-7044460 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2020 |
publisher | The Company of Biologists Ltd |
record_format | MEDLINE/PubMed |
spelling | pubmed-70444602020-02-27 The absence of SOX2 in the anterior foregut alters the esophagus into trachea and bronchi in both epithelial and mesenchymal components Teramoto, Machiko Sugawara, Ryo Minegishi, Katsura Uchikawa, Masanori Takemoto, Tatsuya Kuroiwa, Atsushi Ishii, Yasuo Kondoh, Hisato Biol Open Research Article In the anterior foregut (AFG) of mouse embryos, the transcription factor SOX2 is expressed in the epithelia of the esophagus and proximal branches of respiratory organs comprising the trachea and bronchi, whereas NKX2.1 is expressed only in the epithelia of respiratory organs. Previous studies using hypomorphic Sox2 alleles have indicated that reduced SOX2 expression causes the esophageal epithelium to display some respiratory organ characteristics. In the present study, we produced mouse embryos with AFG-specific SOX2 deficiency. In the absence of SOX2 expression, a single NKX2.1-expressing epithelial tube connected the pharynx and the stomach, and a pair of bronchi developed in the middle of the tube. Expression patterns of NKX2.1 and SOX9 revealed that the anterior and posterior halves of SOX2-deficient AFG epithelial tubes assumed the characteristics of the trachea and bronchus, respectively. In addition, we found that mesenchymal tissues surrounding the SOX2-deficient NKX2.1-expressing epithelial tube changed to those surrounding the trachea and bronchi in the anterior and posterior halves, as indicated by the arrangement of smooth muscle cells and SOX9-expressing cells and by the expression of Wnt4 (esophagus specific), Tbx4 (respiratory organ specific), and Hoxb6 (distal bronchus specific). The impact of mesenchyme-derived signaling on the early stage of AFG epithelial specification has been indicated. Our study demonstrated an opposite trend where epithelial tissue specification causes concordant changes in mesenchymal tissues, indicating a reciprocity of epithelial-mesenchymal interactions. The Company of Biologists Ltd 2020-02-07 /pmc/articles/PMC7044460/ /pubmed/31988094 http://dx.doi.org/10.1242/bio.048728 Text en © 2020. Published by The Company of Biologists Ltd http://creativecommons.org/licenses/by/4.0This is an Open Access article distributed under the terms of the Creative Commons Attribution License (https://creativecommons.org/licenses/by/4.0), which permits unrestricted use, distribution and reproduction in any medium provided that the original work is properly attributed. |
spellingShingle | Research Article Teramoto, Machiko Sugawara, Ryo Minegishi, Katsura Uchikawa, Masanori Takemoto, Tatsuya Kuroiwa, Atsushi Ishii, Yasuo Kondoh, Hisato The absence of SOX2 in the anterior foregut alters the esophagus into trachea and bronchi in both epithelial and mesenchymal components |
title | The absence of SOX2 in the anterior foregut alters the esophagus into trachea and bronchi in both epithelial and mesenchymal components |
title_full | The absence of SOX2 in the anterior foregut alters the esophagus into trachea and bronchi in both epithelial and mesenchymal components |
title_fullStr | The absence of SOX2 in the anterior foregut alters the esophagus into trachea and bronchi in both epithelial and mesenchymal components |
title_full_unstemmed | The absence of SOX2 in the anterior foregut alters the esophagus into trachea and bronchi in both epithelial and mesenchymal components |
title_short | The absence of SOX2 in the anterior foregut alters the esophagus into trachea and bronchi in both epithelial and mesenchymal components |
title_sort | absence of sox2 in the anterior foregut alters the esophagus into trachea and bronchi in both epithelial and mesenchymal components |
topic | Research Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7044460/ https://www.ncbi.nlm.nih.gov/pubmed/31988094 http://dx.doi.org/10.1242/bio.048728 |
work_keys_str_mv | AT teramotomachiko theabsenceofsox2intheanteriorforegutalterstheesophagusintotracheaandbronchiinbothepithelialandmesenchymalcomponents AT sugawararyo theabsenceofsox2intheanteriorforegutalterstheesophagusintotracheaandbronchiinbothepithelialandmesenchymalcomponents AT minegishikatsura theabsenceofsox2intheanteriorforegutalterstheesophagusintotracheaandbronchiinbothepithelialandmesenchymalcomponents AT uchikawamasanori theabsenceofsox2intheanteriorforegutalterstheesophagusintotracheaandbronchiinbothepithelialandmesenchymalcomponents AT takemototatsuya theabsenceofsox2intheanteriorforegutalterstheesophagusintotracheaandbronchiinbothepithelialandmesenchymalcomponents AT kuroiwaatsushi theabsenceofsox2intheanteriorforegutalterstheesophagusintotracheaandbronchiinbothepithelialandmesenchymalcomponents AT ishiiyasuo theabsenceofsox2intheanteriorforegutalterstheesophagusintotracheaandbronchiinbothepithelialandmesenchymalcomponents AT kondohhisato theabsenceofsox2intheanteriorforegutalterstheesophagusintotracheaandbronchiinbothepithelialandmesenchymalcomponents AT teramotomachiko absenceofsox2intheanteriorforegutalterstheesophagusintotracheaandbronchiinbothepithelialandmesenchymalcomponents AT sugawararyo absenceofsox2intheanteriorforegutalterstheesophagusintotracheaandbronchiinbothepithelialandmesenchymalcomponents AT minegishikatsura absenceofsox2intheanteriorforegutalterstheesophagusintotracheaandbronchiinbothepithelialandmesenchymalcomponents AT uchikawamasanori absenceofsox2intheanteriorforegutalterstheesophagusintotracheaandbronchiinbothepithelialandmesenchymalcomponents AT takemototatsuya absenceofsox2intheanteriorforegutalterstheesophagusintotracheaandbronchiinbothepithelialandmesenchymalcomponents AT kuroiwaatsushi absenceofsox2intheanteriorforegutalterstheesophagusintotracheaandbronchiinbothepithelialandmesenchymalcomponents AT ishiiyasuo absenceofsox2intheanteriorforegutalterstheesophagusintotracheaandbronchiinbothepithelialandmesenchymalcomponents AT kondohhisato absenceofsox2intheanteriorforegutalterstheesophagusintotracheaandbronchiinbothepithelialandmesenchymalcomponents |