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Sensing Peroxynitrite in Different Organelles of Murine RAW264.7 Macrophages With Coumarin-Based Fluorescent Probes
The elucidation of biological processes involving reactive oxygen species (ROS) facilitates a better understanding of the underlying progression of non-communicable diseases. Fluorescent probes are a powerful tool to study various ROS and have the potential to become essential diagnostic tools. We h...
Autores principales: | , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Frontiers Media S.A.
2020
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7044669/ https://www.ncbi.nlm.nih.gov/pubmed/32154211 http://dx.doi.org/10.3389/fchem.2020.00039 |
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author | Weber, Maria Yamada, Namiko Tian, Xue Bull, Steven D. Minoshima, Masafumi Kikuchi, Kazuya Mackenzie, Amanda B. James, Tony D. |
author_facet | Weber, Maria Yamada, Namiko Tian, Xue Bull, Steven D. Minoshima, Masafumi Kikuchi, Kazuya Mackenzie, Amanda B. James, Tony D. |
author_sort | Weber, Maria |
collection | PubMed |
description | The elucidation of biological processes involving reactive oxygen species (ROS) facilitates a better understanding of the underlying progression of non-communicable diseases. Fluorescent probes are a powerful tool to study various ROS and have the potential to become essential diagnostic tools. We have developed a series of coumarin fluorescent probes for the selective and sensitive detection of peroxynitrite (ONOO(−)), a key ROS. Coumarin based probes exhibit good photostability, large Stokes shift and high quantum yields. The three ratiometric probes all contain a boronate ester motif for the detection of ONOO(−) and a distinctive organelle targeting group. The study of ONOO(−) generation in a particular organelle will allow more precise disease profiling. Hence, targeting groups for the mitochondria, lysosome and endoplasmic reticulum were introduced into a coumarin scaffold. The three ratiometric probes displayed sensitive and selective detection of ONOO(−) over other ROS species. All three coumarin probes were evaluated in murine RAW264.7 macrophages for detection of basal and stimulated ONOO(−) formation. |
format | Online Article Text |
id | pubmed-7044669 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2020 |
publisher | Frontiers Media S.A. |
record_format | MEDLINE/PubMed |
spelling | pubmed-70446692020-03-09 Sensing Peroxynitrite in Different Organelles of Murine RAW264.7 Macrophages With Coumarin-Based Fluorescent Probes Weber, Maria Yamada, Namiko Tian, Xue Bull, Steven D. Minoshima, Masafumi Kikuchi, Kazuya Mackenzie, Amanda B. James, Tony D. Front Chem Chemistry The elucidation of biological processes involving reactive oxygen species (ROS) facilitates a better understanding of the underlying progression of non-communicable diseases. Fluorescent probes are a powerful tool to study various ROS and have the potential to become essential diagnostic tools. We have developed a series of coumarin fluorescent probes for the selective and sensitive detection of peroxynitrite (ONOO(−)), a key ROS. Coumarin based probes exhibit good photostability, large Stokes shift and high quantum yields. The three ratiometric probes all contain a boronate ester motif for the detection of ONOO(−) and a distinctive organelle targeting group. The study of ONOO(−) generation in a particular organelle will allow more precise disease profiling. Hence, targeting groups for the mitochondria, lysosome and endoplasmic reticulum were introduced into a coumarin scaffold. The three ratiometric probes displayed sensitive and selective detection of ONOO(−) over other ROS species. All three coumarin probes were evaluated in murine RAW264.7 macrophages for detection of basal and stimulated ONOO(−) formation. Frontiers Media S.A. 2020-02-20 /pmc/articles/PMC7044669/ /pubmed/32154211 http://dx.doi.org/10.3389/fchem.2020.00039 Text en Copyright © 2020 Weber, Yamada, Tian, Bull, Minoshima, Kikuchi, Mackenzie and James. http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms. |
spellingShingle | Chemistry Weber, Maria Yamada, Namiko Tian, Xue Bull, Steven D. Minoshima, Masafumi Kikuchi, Kazuya Mackenzie, Amanda B. James, Tony D. Sensing Peroxynitrite in Different Organelles of Murine RAW264.7 Macrophages With Coumarin-Based Fluorescent Probes |
title | Sensing Peroxynitrite in Different Organelles of Murine RAW264.7 Macrophages With Coumarin-Based Fluorescent Probes |
title_full | Sensing Peroxynitrite in Different Organelles of Murine RAW264.7 Macrophages With Coumarin-Based Fluorescent Probes |
title_fullStr | Sensing Peroxynitrite in Different Organelles of Murine RAW264.7 Macrophages With Coumarin-Based Fluorescent Probes |
title_full_unstemmed | Sensing Peroxynitrite in Different Organelles of Murine RAW264.7 Macrophages With Coumarin-Based Fluorescent Probes |
title_short | Sensing Peroxynitrite in Different Organelles of Murine RAW264.7 Macrophages With Coumarin-Based Fluorescent Probes |
title_sort | sensing peroxynitrite in different organelles of murine raw264.7 macrophages with coumarin-based fluorescent probes |
topic | Chemistry |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7044669/ https://www.ncbi.nlm.nih.gov/pubmed/32154211 http://dx.doi.org/10.3389/fchem.2020.00039 |
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