Stable Isotope Marking of Laboratory-Reared Aedes aegypti (Diptera: Culicidae)

The use of stable isotope enrichment to mark mosquitoes has provided a tool to study the biology of vector species. In this study, we evaluated isotopic marking of Aedes aegypti (L.) (Diptera: Culicidae) in a laboratory setting. We determined the optimal dosage for marking adult Ae. aegypti mosquito...

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Autores principales: Garcia-Luna, Selene M, Juarez, Jose G, Cabañas, Sofia, Tang, Wendy, Roark, E Brendan, Maupin, Christopher R, Badillo-Vargas, Ismael E, Hamer, Gabriel L
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Oxford University Press 2020
Materias:
Short Communications
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7044722/
https://www.ncbi.nlm.nih.gov/pubmed/31751467
http://dx.doi.org/10.1093/jme/tjz210
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author Garcia-Luna, Selene M
Juarez, Jose G
Cabañas, Sofia
Tang, Wendy
Roark, E Brendan
Maupin, Christopher R
Badillo-Vargas, Ismael E
Hamer, Gabriel L
author_facet Garcia-Luna, Selene M
Juarez, Jose G
Cabañas, Sofia
Tang, Wendy
Roark, E Brendan
Maupin, Christopher R
Badillo-Vargas, Ismael E
Hamer, Gabriel L
author_sort Garcia-Luna, Selene M
collection PubMed
description The use of stable isotope enrichment to mark mosquitoes has provided a tool to study the biology of vector species. In this study, we evaluated isotopic marking of Aedes aegypti (L.) (Diptera: Culicidae) in a laboratory setting. We determined the optimal dosage for marking adult Ae. aegypti mosquitoes with (13)C and (15)N. Additionally, Ae. aegypti mosquitoes were single and dually marked with (13)C and (15)N for up to 60 d postemergence without changes to adult body size or transgenerational marking. This report adds to the growing literature that explores the use of alternative marking methods for ecological and vector biology studies.
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spelling pubmed-70447222020-03-03 Stable Isotope Marking of Laboratory-Reared Aedes aegypti (Diptera: Culicidae) Garcia-Luna, Selene M Juarez, Jose G Cabañas, Sofia Tang, Wendy Roark, E Brendan Maupin, Christopher R Badillo-Vargas, Ismael E Hamer, Gabriel L J Med Entomol Short Communications The use of stable isotope enrichment to mark mosquitoes has provided a tool to study the biology of vector species. In this study, we evaluated isotopic marking of Aedes aegypti (L.) (Diptera: Culicidae) in a laboratory setting. We determined the optimal dosage for marking adult Ae. aegypti mosquitoes with (13)C and (15)N. Additionally, Ae. aegypti mosquitoes were single and dually marked with (13)C and (15)N for up to 60 d postemergence without changes to adult body size or transgenerational marking. This report adds to the growing literature that explores the use of alternative marking methods for ecological and vector biology studies. Oxford University Press 2020-02 2019-11-21 /pmc/articles/PMC7044722/ /pubmed/31751467 http://dx.doi.org/10.1093/jme/tjz210 Text en © The Author(s) 2019. Published by Oxford University Press on behalf of Entomological Society of America. http://creativecommons.org/licenses/by/4.0/ This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted reuse, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Short Communications
Garcia-Luna, Selene M
Juarez, Jose G
Cabañas, Sofia
Tang, Wendy
Roark, E Brendan
Maupin, Christopher R
Badillo-Vargas, Ismael E
Hamer, Gabriel L
Stable Isotope Marking of Laboratory-Reared Aedes aegypti (Diptera: Culicidae)
title Stable Isotope Marking of Laboratory-Reared Aedes aegypti (Diptera: Culicidae)
title_full Stable Isotope Marking of Laboratory-Reared Aedes aegypti (Diptera: Culicidae)
title_fullStr Stable Isotope Marking of Laboratory-Reared Aedes aegypti (Diptera: Culicidae)
title_full_unstemmed Stable Isotope Marking of Laboratory-Reared Aedes aegypti (Diptera: Culicidae)
title_short Stable Isotope Marking of Laboratory-Reared Aedes aegypti (Diptera: Culicidae)
title_sort stable isotope marking of laboratory-reared aedes aegypti (diptera: culicidae)
topic Short Communications
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7044722/
https://www.ncbi.nlm.nih.gov/pubmed/31751467
http://dx.doi.org/10.1093/jme/tjz210
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