Cell Membrane and Electron Transfer Engineering for Improved Synthesis of Menaquinone-7 in Bacillus subtilis

The formation of biofilm facilitates the synthesis of valuable natural product menaquinone-7 (MK-7) in static culture of Bacillus subtilis, whereas the essential role and mechanism of biofilm in MK-7 synthesis have not been revealed. Herein, comparative transcriptomics show that the formation of bio...

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Autores principales: Cui, Shixiu, Xia, Hongzhi, Chen, Taichi, Gu, Yang, Lv, Xueqin, Liu, Yanfeng, Li, Jianghua, Du, Guocheng, Liu, Long
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Elsevier 2020
Materias:
Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7044751/
https://www.ncbi.nlm.nih.gov/pubmed/32109677
http://dx.doi.org/10.1016/j.isci.2020.100918
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author Cui, Shixiu
Xia, Hongzhi
Chen, Taichi
Gu, Yang
Lv, Xueqin
Liu, Yanfeng
Li, Jianghua
Du, Guocheng
Liu, Long
author_facet Cui, Shixiu
Xia, Hongzhi
Chen, Taichi
Gu, Yang
Lv, Xueqin
Liu, Yanfeng
Li, Jianghua
Du, Guocheng
Liu, Long
author_sort Cui, Shixiu
collection PubMed
description The formation of biofilm facilitates the synthesis of valuable natural product menaquinone-7 (MK-7) in static culture of Bacillus subtilis, whereas the essential role and mechanism of biofilm in MK-7 synthesis have not been revealed. Herein, comparative transcriptomics show that the formation of biofilm affected MK-7 synthesis by changing the transcription levels of signal receptor (BSU02010), transmembrane transporter (BSU29340, BSU03070), and signal transduction (BSU02630). Moreover, we also found that oxalate decarboxylase OxdC has an important effect on electron generation and MK-7 synthesis, when the transcriptional level of NADH dehydrogenase decreases in static culture. Our results revealed that cell membrane and electron transfer are important factors in promoting MK-7 synthesis.
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spelling pubmed-70447512020-03-05 Cell Membrane and Electron Transfer Engineering for Improved Synthesis of Menaquinone-7 in Bacillus subtilis Cui, Shixiu Xia, Hongzhi Chen, Taichi Gu, Yang Lv, Xueqin Liu, Yanfeng Li, Jianghua Du, Guocheng Liu, Long iScience Article The formation of biofilm facilitates the synthesis of valuable natural product menaquinone-7 (MK-7) in static culture of Bacillus subtilis, whereas the essential role and mechanism of biofilm in MK-7 synthesis have not been revealed. Herein, comparative transcriptomics show that the formation of biofilm affected MK-7 synthesis by changing the transcription levels of signal receptor (BSU02010), transmembrane transporter (BSU29340, BSU03070), and signal transduction (BSU02630). Moreover, we also found that oxalate decarboxylase OxdC has an important effect on electron generation and MK-7 synthesis, when the transcriptional level of NADH dehydrogenase decreases in static culture. Our results revealed that cell membrane and electron transfer are important factors in promoting MK-7 synthesis. Elsevier 2020-02-14 /pmc/articles/PMC7044751/ /pubmed/32109677 http://dx.doi.org/10.1016/j.isci.2020.100918 Text en © 2020 The Author(s) http://creativecommons.org/licenses/by-nc-nd/4.0/ This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/).
spellingShingle Article
Cui, Shixiu
Xia, Hongzhi
Chen, Taichi
Gu, Yang
Lv, Xueqin
Liu, Yanfeng
Li, Jianghua
Du, Guocheng
Liu, Long
Cell Membrane and Electron Transfer Engineering for Improved Synthesis of Menaquinone-7 in Bacillus subtilis
title Cell Membrane and Electron Transfer Engineering for Improved Synthesis of Menaquinone-7 in Bacillus subtilis
title_full Cell Membrane and Electron Transfer Engineering for Improved Synthesis of Menaquinone-7 in Bacillus subtilis
title_fullStr Cell Membrane and Electron Transfer Engineering for Improved Synthesis of Menaquinone-7 in Bacillus subtilis
title_full_unstemmed Cell Membrane and Electron Transfer Engineering for Improved Synthesis of Menaquinone-7 in Bacillus subtilis
title_short Cell Membrane and Electron Transfer Engineering for Improved Synthesis of Menaquinone-7 in Bacillus subtilis
title_sort cell membrane and electron transfer engineering for improved synthesis of menaquinone-7 in bacillus subtilis
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7044751/
https://www.ncbi.nlm.nih.gov/pubmed/32109677
http://dx.doi.org/10.1016/j.isci.2020.100918
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