Generation of Rat Monoclonal Antibodies Specific for Human Stromal Cell-Derived Factor-2

Stromal cell-derived factor-2 (SDF-2) is reportedly involved in multiple endoplasmic reticulum (ER) functions, including the misfolded protein catabolic process, protein glycosylation, and ER protein quality control. However, the precise molecular and cellular functions of SDF-2 remain unknown. Prev...

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Autores principales: Tanaka, Masako, Shiota, Masayuki, Koyama, Masaru, Nakayama, Jun, Yashiro, Masakazu, Semba, Kentaro, Goda, Nobuhito
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Mary Ann Liebert, Inc., publishers 2020
Materias:
Short Communication
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7044778/
https://www.ncbi.nlm.nih.gov/pubmed/31916900
http://dx.doi.org/10.1089/mab.2019.0043
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author Tanaka, Masako
Shiota, Masayuki
Koyama, Masaru
Nakayama, Jun
Yashiro, Masakazu
Semba, Kentaro
Goda, Nobuhito
author_facet Tanaka, Masako
Shiota, Masayuki
Koyama, Masaru
Nakayama, Jun
Yashiro, Masakazu
Semba, Kentaro
Goda, Nobuhito
author_sort Tanaka, Masako
collection PubMed
description Stromal cell-derived factor-2 (SDF-2) is reportedly involved in multiple endoplasmic reticulum (ER) functions, including the misfolded protein catabolic process, protein glycosylation, and ER protein quality control. However, the precise molecular and cellular functions of SDF-2 remain unknown. Previously, we discovered that SDF-2 mediates acquired resistance to oxaliplatin in human gastric cancer cells. In this study, we have generated SDF-2-specific monoclonal antibodies (mAbs), using the rat medial iliac lymph node method, as a tool to explore novel mechanisms of oxaliplatin resistance. The antibodies detected endogenous human SDF-2 in immunoblotting analyses. In addition, immunoprecipitation analyses revealed the availability of these antibodies for human SDF-2. Thus, these mAbs will be available to elucidate molecular and cellular functions of SDF-2 in cancer cells.
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spelling pubmed-70447782020-02-27 Generation of Rat Monoclonal Antibodies Specific for Human Stromal Cell-Derived Factor-2 Tanaka, Masako Shiota, Masayuki Koyama, Masaru Nakayama, Jun Yashiro, Masakazu Semba, Kentaro Goda, Nobuhito Monoclon Antib Immunodiagn Immunother Short Communication Stromal cell-derived factor-2 (SDF-2) is reportedly involved in multiple endoplasmic reticulum (ER) functions, including the misfolded protein catabolic process, protein glycosylation, and ER protein quality control. However, the precise molecular and cellular functions of SDF-2 remain unknown. Previously, we discovered that SDF-2 mediates acquired resistance to oxaliplatin in human gastric cancer cells. In this study, we have generated SDF-2-specific monoclonal antibodies (mAbs), using the rat medial iliac lymph node method, as a tool to explore novel mechanisms of oxaliplatin resistance. The antibodies detected endogenous human SDF-2 in immunoblotting analyses. In addition, immunoprecipitation analyses revealed the availability of these antibodies for human SDF-2. Thus, these mAbs will be available to elucidate molecular and cellular functions of SDF-2 in cancer cells. Mary Ann Liebert, Inc., publishers 2020-02-01 2020-02-14 /pmc/articles/PMC7044778/ /pubmed/31916900 http://dx.doi.org/10.1089/mab.2019.0043 Text en © Masako Tanaka et al. 2020; Published by Mary Ann Liebert, Inc. This Open Access article is distributed under the terms of the Creative Commons Attribution Noncommercial License (http://creativecommons.org/licenses/by-nc/4.0/) which permits any noncommercial use, distribution, and reproduction in any medium, provided the original author(s) and the source are cited.
spellingShingle Short Communication
Tanaka, Masako
Shiota, Masayuki
Koyama, Masaru
Nakayama, Jun
Yashiro, Masakazu
Semba, Kentaro
Goda, Nobuhito
Generation of Rat Monoclonal Antibodies Specific for Human Stromal Cell-Derived Factor-2
title Generation of Rat Monoclonal Antibodies Specific for Human Stromal Cell-Derived Factor-2
title_full Generation of Rat Monoclonal Antibodies Specific for Human Stromal Cell-Derived Factor-2
title_fullStr Generation of Rat Monoclonal Antibodies Specific for Human Stromal Cell-Derived Factor-2
title_full_unstemmed Generation of Rat Monoclonal Antibodies Specific for Human Stromal Cell-Derived Factor-2
title_short Generation of Rat Monoclonal Antibodies Specific for Human Stromal Cell-Derived Factor-2
title_sort generation of rat monoclonal antibodies specific for human stromal cell-derived factor-2
topic Short Communication
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7044778/
https://www.ncbi.nlm.nih.gov/pubmed/31916900
http://dx.doi.org/10.1089/mab.2019.0043
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