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The necessity for standardization of glioma stem cell culture: a systematic review

BACKGROUND: The cancer stem cell hypothesis is an old idea which has been revived in recent years for many cancers, including gliomas. However, this concept has become controversial due to a series of studies with conflicting results. METHODS: A systematic literature search was conducted in PubMed a...

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Autores principales: Zhang, Lei, Yu, Hongwei, Yuan, Yuhui, Yu, John S., Lou, Zhenkun, Xue, Yixue, Liu, Yunhui
Formato: Online Artículo Texto
Lenguaje:English
Publicado: BioMed Central 2020
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7045630/
https://www.ncbi.nlm.nih.gov/pubmed/32102678
http://dx.doi.org/10.1186/s13287-020-01589-8
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author Zhang, Lei
Yu, Hongwei
Yuan, Yuhui
Yu, John S.
Lou, Zhenkun
Xue, Yixue
Liu, Yunhui
author_facet Zhang, Lei
Yu, Hongwei
Yuan, Yuhui
Yu, John S.
Lou, Zhenkun
Xue, Yixue
Liu, Yunhui
author_sort Zhang, Lei
collection PubMed
description BACKGROUND: The cancer stem cell hypothesis is an old idea which has been revived in recent years for many cancers, including gliomas. However, this concept has become controversial due to a series of studies with conflicting results. METHODS: A systematic literature search was conducted in PubMed and the Web of Science database to analyze studies using serum-free medium and its components in glioma stem cells, glioma stem-like cells, glioma-initiating cells, or glioma neurosphere cultures. All the studies reviewed were published between 1970 and 2019. We found that no standardized culture method was used, and the data were incomparable due to differing culture conditions and the use of media with different components. CONCLUSIONS: Here, we review the most commonly used serum-free media and added components for glioma stem cell culture while highlighting the function of each component used in the media. We emphasize the necessity for standardization of glioma stem cell culture and propose a standard culture medium to prevent bias in glioma stem cell research.
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spelling pubmed-70456302020-03-03 The necessity for standardization of glioma stem cell culture: a systematic review Zhang, Lei Yu, Hongwei Yuan, Yuhui Yu, John S. Lou, Zhenkun Xue, Yixue Liu, Yunhui Stem Cell Res Ther Review BACKGROUND: The cancer stem cell hypothesis is an old idea which has been revived in recent years for many cancers, including gliomas. However, this concept has become controversial due to a series of studies with conflicting results. METHODS: A systematic literature search was conducted in PubMed and the Web of Science database to analyze studies using serum-free medium and its components in glioma stem cells, glioma stem-like cells, glioma-initiating cells, or glioma neurosphere cultures. All the studies reviewed were published between 1970 and 2019. We found that no standardized culture method was used, and the data were incomparable due to differing culture conditions and the use of media with different components. CONCLUSIONS: Here, we review the most commonly used serum-free media and added components for glioma stem cell culture while highlighting the function of each component used in the media. We emphasize the necessity for standardization of glioma stem cell culture and propose a standard culture medium to prevent bias in glioma stem cell research. BioMed Central 2020-02-26 /pmc/articles/PMC7045630/ /pubmed/32102678 http://dx.doi.org/10.1186/s13287-020-01589-8 Text en © The Author(s) 2020 Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.
spellingShingle Review
Zhang, Lei
Yu, Hongwei
Yuan, Yuhui
Yu, John S.
Lou, Zhenkun
Xue, Yixue
Liu, Yunhui
The necessity for standardization of glioma stem cell culture: a systematic review
title The necessity for standardization of glioma stem cell culture: a systematic review
title_full The necessity for standardization of glioma stem cell culture: a systematic review
title_fullStr The necessity for standardization of glioma stem cell culture: a systematic review
title_full_unstemmed The necessity for standardization of glioma stem cell culture: a systematic review
title_short The necessity for standardization of glioma stem cell culture: a systematic review
title_sort necessity for standardization of glioma stem cell culture: a systematic review
topic Review
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7045630/
https://www.ncbi.nlm.nih.gov/pubmed/32102678
http://dx.doi.org/10.1186/s13287-020-01589-8
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