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Anti-inflammatory effect of adipose-derived stem cells in the treatment of pelvic organ prolapse

OBJECTIVE: This study investigated the effect of recombinant human connective tissue growth factor (hCTGF) on rat adipose-derived stem cells (ADSCs) and explored the feasibility of using ADSCs to treat pelvic organ prolapse. METHODS: ADSCs were isolated from rat inguinal adipose tissue and character...

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Autores principales: Wang, Su, Gao, Jian, Wang, Maohuai, Chen, Liquan, Zhang, Xiaowei, Wang, Xiaoyu
Formato: Online Artículo Texto
Lenguaje:English
Publicado: SAGE Publications 2019
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7045653/
https://www.ncbi.nlm.nih.gov/pubmed/31741397
http://dx.doi.org/10.1177/0300060519873472
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author Wang, Su
Gao, Jian
Wang, Maohuai
Chen, Liquan
Zhang, Xiaowei
Wang, Xiaoyu
author_facet Wang, Su
Gao, Jian
Wang, Maohuai
Chen, Liquan
Zhang, Xiaowei
Wang, Xiaoyu
author_sort Wang, Su
collection PubMed
description OBJECTIVE: This study investigated the effect of recombinant human connective tissue growth factor (hCTGF) on rat adipose-derived stem cells (ADSCs) and explored the feasibility of using ADSCs to treat pelvic organ prolapse. METHODS: ADSCs were isolated from rat inguinal adipose tissue and characterized by flow cytometry and for osteogenic and adipogenic differentiation. ADSCs were treated with recombinant hCTGF and qRT-PCR was performed to detect collagen I and III expression on post-treatment days 7, 14, and 28. Osteogenic and adipogenic differentiation of ADSCs was performed to evaluate the effect of hCTGF. ADSCs were seeded in biological grafting materials, acellular porcine pericardium (APP) and acellular bovine pericardium (ABP), then implanted in the rat vagina. Histology was performed to observe inflammation among different groups. RESULTS: Collagen I and III expression in ADSCs was significantly increased, and the ability to differentiate into osteogenic and adipogenic lineages was diminished after hCTGF treatment. APP and ABP seeded with ADSCs significantly decreased inflammation and protected from degradation in vivo compared with APP and ABP only; ABP seeded with ADSCs had the lowest inflammation. CONCLUSION: hCTGF regulates collagen I and III expression and induces ADSC differentiation in vitro. ADSCs decrease inflammation associated with APP and ABP in vivo.
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spelling pubmed-70456532020-03-09 Anti-inflammatory effect of adipose-derived stem cells in the treatment of pelvic organ prolapse Wang, Su Gao, Jian Wang, Maohuai Chen, Liquan Zhang, Xiaowei Wang, Xiaoyu J Int Med Res Pre-Clinical Research Reports OBJECTIVE: This study investigated the effect of recombinant human connective tissue growth factor (hCTGF) on rat adipose-derived stem cells (ADSCs) and explored the feasibility of using ADSCs to treat pelvic organ prolapse. METHODS: ADSCs were isolated from rat inguinal adipose tissue and characterized by flow cytometry and for osteogenic and adipogenic differentiation. ADSCs were treated with recombinant hCTGF and qRT-PCR was performed to detect collagen I and III expression on post-treatment days 7, 14, and 28. Osteogenic and adipogenic differentiation of ADSCs was performed to evaluate the effect of hCTGF. ADSCs were seeded in biological grafting materials, acellular porcine pericardium (APP) and acellular bovine pericardium (ABP), then implanted in the rat vagina. Histology was performed to observe inflammation among different groups. RESULTS: Collagen I and III expression in ADSCs was significantly increased, and the ability to differentiate into osteogenic and adipogenic lineages was diminished after hCTGF treatment. APP and ABP seeded with ADSCs significantly decreased inflammation and protected from degradation in vivo compared with APP and ABP only; ABP seeded with ADSCs had the lowest inflammation. CONCLUSION: hCTGF regulates collagen I and III expression and induces ADSC differentiation in vitro. ADSCs decrease inflammation associated with APP and ABP in vivo. SAGE Publications 2019-11-19 2019-12 /pmc/articles/PMC7045653/ /pubmed/31741397 http://dx.doi.org/10.1177/0300060519873472 Text en © The Author(s) 2019 http://creativecommons.org/licenses/by-nc/4.0/ Creative Commons Non Commercial CC BY-NC: This article is distributed under the terms of the Creative Commons Attribution-NonCommercial 4.0 License (http://www.creativecommons.org/licenses/by-nc/4.0/) which permits non-commercial use, reproduction and distribution of the work without further permission provided the original work is attributed as specified on the SAGE and Open Access pages (https://us.sagepub.com/en-us/nam/open-access-at-sage).
spellingShingle Pre-Clinical Research Reports
Wang, Su
Gao, Jian
Wang, Maohuai
Chen, Liquan
Zhang, Xiaowei
Wang, Xiaoyu
Anti-inflammatory effect of adipose-derived stem cells in the treatment of pelvic organ prolapse
title Anti-inflammatory effect of adipose-derived stem cells in the treatment of pelvic organ prolapse
title_full Anti-inflammatory effect of adipose-derived stem cells in the treatment of pelvic organ prolapse
title_fullStr Anti-inflammatory effect of adipose-derived stem cells in the treatment of pelvic organ prolapse
title_full_unstemmed Anti-inflammatory effect of adipose-derived stem cells in the treatment of pelvic organ prolapse
title_short Anti-inflammatory effect of adipose-derived stem cells in the treatment of pelvic organ prolapse
title_sort anti-inflammatory effect of adipose-derived stem cells in the treatment of pelvic organ prolapse
topic Pre-Clinical Research Reports
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7045653/
https://www.ncbi.nlm.nih.gov/pubmed/31741397
http://dx.doi.org/10.1177/0300060519873472
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