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Isolation of a Novel Phage and Targeting Biofilms of Drug-Resistant Oral Enterococci

INTRODUCTION: Enterococci are now recognized as the second most cause of nosocomial infections worldwide. The emergence of multidrug-resistant strains in the organism has given rise to alternative strategies such as phage therapy. In this study, an Enterococcus faecalis infecting phage was isolated...

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Autores principales: Bhardwaj, Sonia Bhonchal, Mehta, Manjula, Sood, Shaveta, Sharma, Jyoti
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Wolters Kluwer - Medknow 2020
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7045759/
https://www.ncbi.nlm.nih.gov/pubmed/32165796
http://dx.doi.org/10.4103/jgid.jgid_110_19
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author Bhardwaj, Sonia Bhonchal
Mehta, Manjula
Sood, Shaveta
Sharma, Jyoti
author_facet Bhardwaj, Sonia Bhonchal
Mehta, Manjula
Sood, Shaveta
Sharma, Jyoti
author_sort Bhardwaj, Sonia Bhonchal
collection PubMed
description INTRODUCTION: Enterococci are now recognized as the second most cause of nosocomial infections worldwide. The emergence of multidrug-resistant strains in the organism has given rise to alternative strategies such as phage therapy. In this study, an Enterococcus faecalis infecting phage was isolated and its efficiency against biofilms formed by drug-resistant enterococci obtained from chronic periodontitis was evaluated. MATERIALS AND METHODS: Bacteriophage against E. faecalis was isolated from sewage sample. The phage was propagated and identified using transmission electron microscope (TEM). In vitro biofilm formation was assessed. RESULTS: TEM microscopy showed that the phage belonged to Siphoviridae family. In the presence of the novel phage, the metabolic activity of enterococci biofilm was reduced at 48 h of contact. A difference of at least 5 log CFU/ml was seen in the live cells of the control biofilm, and the phage treated biofilm of enterococci isolates. CONCLUSION: The study shows that the novel phage inhibits biofilm production in oral enterococci isolates from periodontitis patients but has a narrow host range. The role of bacteriophages as strong biotechnological and natural therapeutic agents for E. faecalis in chronic periodontitis can be considered.
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spelling pubmed-70457592020-03-12 Isolation of a Novel Phage and Targeting Biofilms of Drug-Resistant Oral Enterococci Bhardwaj, Sonia Bhonchal Mehta, Manjula Sood, Shaveta Sharma, Jyoti J Glob Infect Dis Original Article INTRODUCTION: Enterococci are now recognized as the second most cause of nosocomial infections worldwide. The emergence of multidrug-resistant strains in the organism has given rise to alternative strategies such as phage therapy. In this study, an Enterococcus faecalis infecting phage was isolated and its efficiency against biofilms formed by drug-resistant enterococci obtained from chronic periodontitis was evaluated. MATERIALS AND METHODS: Bacteriophage against E. faecalis was isolated from sewage sample. The phage was propagated and identified using transmission electron microscope (TEM). In vitro biofilm formation was assessed. RESULTS: TEM microscopy showed that the phage belonged to Siphoviridae family. In the presence of the novel phage, the metabolic activity of enterococci biofilm was reduced at 48 h of contact. A difference of at least 5 log CFU/ml was seen in the live cells of the control biofilm, and the phage treated biofilm of enterococci isolates. CONCLUSION: The study shows that the novel phage inhibits biofilm production in oral enterococci isolates from periodontitis patients but has a narrow host range. The role of bacteriophages as strong biotechnological and natural therapeutic agents for E. faecalis in chronic periodontitis can be considered. Wolters Kluwer - Medknow 2020-02-19 /pmc/articles/PMC7045759/ /pubmed/32165796 http://dx.doi.org/10.4103/jgid.jgid_110_19 Text en Copyright: © 2020 Journal of Global Infectious Diseases http://creativecommons.org/licenses/by-nc-sa/4.0 This is an open access journal, and articles are distributed under the terms of the Creative Commons Attribution-NonCommercial-ShareAlike 4.0 License, which allows others to remix, tweak, and build upon the work non-commercially, as long as appropriate credit is given and the new creations are licensed under the identical terms.
spellingShingle Original Article
Bhardwaj, Sonia Bhonchal
Mehta, Manjula
Sood, Shaveta
Sharma, Jyoti
Isolation of a Novel Phage and Targeting Biofilms of Drug-Resistant Oral Enterococci
title Isolation of a Novel Phage and Targeting Biofilms of Drug-Resistant Oral Enterococci
title_full Isolation of a Novel Phage and Targeting Biofilms of Drug-Resistant Oral Enterococci
title_fullStr Isolation of a Novel Phage and Targeting Biofilms of Drug-Resistant Oral Enterococci
title_full_unstemmed Isolation of a Novel Phage and Targeting Biofilms of Drug-Resistant Oral Enterococci
title_short Isolation of a Novel Phage and Targeting Biofilms of Drug-Resistant Oral Enterococci
title_sort isolation of a novel phage and targeting biofilms of drug-resistant oral enterococci
topic Original Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7045759/
https://www.ncbi.nlm.nih.gov/pubmed/32165796
http://dx.doi.org/10.4103/jgid.jgid_110_19
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