Cargando…
Isolation of a Novel Phage and Targeting Biofilms of Drug-Resistant Oral Enterococci
INTRODUCTION: Enterococci are now recognized as the second most cause of nosocomial infections worldwide. The emergence of multidrug-resistant strains in the organism has given rise to alternative strategies such as phage therapy. In this study, an Enterococcus faecalis infecting phage was isolated...
Autores principales: | , , , |
---|---|
Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Wolters Kluwer - Medknow
2020
|
Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7045759/ https://www.ncbi.nlm.nih.gov/pubmed/32165796 http://dx.doi.org/10.4103/jgid.jgid_110_19 |
_version_ | 1783501840199974912 |
---|---|
author | Bhardwaj, Sonia Bhonchal Mehta, Manjula Sood, Shaveta Sharma, Jyoti |
author_facet | Bhardwaj, Sonia Bhonchal Mehta, Manjula Sood, Shaveta Sharma, Jyoti |
author_sort | Bhardwaj, Sonia Bhonchal |
collection | PubMed |
description | INTRODUCTION: Enterococci are now recognized as the second most cause of nosocomial infections worldwide. The emergence of multidrug-resistant strains in the organism has given rise to alternative strategies such as phage therapy. In this study, an Enterococcus faecalis infecting phage was isolated and its efficiency against biofilms formed by drug-resistant enterococci obtained from chronic periodontitis was evaluated. MATERIALS AND METHODS: Bacteriophage against E. faecalis was isolated from sewage sample. The phage was propagated and identified using transmission electron microscope (TEM). In vitro biofilm formation was assessed. RESULTS: TEM microscopy showed that the phage belonged to Siphoviridae family. In the presence of the novel phage, the metabolic activity of enterococci biofilm was reduced at 48 h of contact. A difference of at least 5 log CFU/ml was seen in the live cells of the control biofilm, and the phage treated biofilm of enterococci isolates. CONCLUSION: The study shows that the novel phage inhibits biofilm production in oral enterococci isolates from periodontitis patients but has a narrow host range. The role of bacteriophages as strong biotechnological and natural therapeutic agents for E. faecalis in chronic periodontitis can be considered. |
format | Online Article Text |
id | pubmed-7045759 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2020 |
publisher | Wolters Kluwer - Medknow |
record_format | MEDLINE/PubMed |
spelling | pubmed-70457592020-03-12 Isolation of a Novel Phage and Targeting Biofilms of Drug-Resistant Oral Enterococci Bhardwaj, Sonia Bhonchal Mehta, Manjula Sood, Shaveta Sharma, Jyoti J Glob Infect Dis Original Article INTRODUCTION: Enterococci are now recognized as the second most cause of nosocomial infections worldwide. The emergence of multidrug-resistant strains in the organism has given rise to alternative strategies such as phage therapy. In this study, an Enterococcus faecalis infecting phage was isolated and its efficiency against biofilms formed by drug-resistant enterococci obtained from chronic periodontitis was evaluated. MATERIALS AND METHODS: Bacteriophage against E. faecalis was isolated from sewage sample. The phage was propagated and identified using transmission electron microscope (TEM). In vitro biofilm formation was assessed. RESULTS: TEM microscopy showed that the phage belonged to Siphoviridae family. In the presence of the novel phage, the metabolic activity of enterococci biofilm was reduced at 48 h of contact. A difference of at least 5 log CFU/ml was seen in the live cells of the control biofilm, and the phage treated biofilm of enterococci isolates. CONCLUSION: The study shows that the novel phage inhibits biofilm production in oral enterococci isolates from periodontitis patients but has a narrow host range. The role of bacteriophages as strong biotechnological and natural therapeutic agents for E. faecalis in chronic periodontitis can be considered. Wolters Kluwer - Medknow 2020-02-19 /pmc/articles/PMC7045759/ /pubmed/32165796 http://dx.doi.org/10.4103/jgid.jgid_110_19 Text en Copyright: © 2020 Journal of Global Infectious Diseases http://creativecommons.org/licenses/by-nc-sa/4.0 This is an open access journal, and articles are distributed under the terms of the Creative Commons Attribution-NonCommercial-ShareAlike 4.0 License, which allows others to remix, tweak, and build upon the work non-commercially, as long as appropriate credit is given and the new creations are licensed under the identical terms. |
spellingShingle | Original Article Bhardwaj, Sonia Bhonchal Mehta, Manjula Sood, Shaveta Sharma, Jyoti Isolation of a Novel Phage and Targeting Biofilms of Drug-Resistant Oral Enterococci |
title | Isolation of a Novel Phage and Targeting Biofilms of Drug-Resistant Oral Enterococci |
title_full | Isolation of a Novel Phage and Targeting Biofilms of Drug-Resistant Oral Enterococci |
title_fullStr | Isolation of a Novel Phage and Targeting Biofilms of Drug-Resistant Oral Enterococci |
title_full_unstemmed | Isolation of a Novel Phage and Targeting Biofilms of Drug-Resistant Oral Enterococci |
title_short | Isolation of a Novel Phage and Targeting Biofilms of Drug-Resistant Oral Enterococci |
title_sort | isolation of a novel phage and targeting biofilms of drug-resistant oral enterococci |
topic | Original Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7045759/ https://www.ncbi.nlm.nih.gov/pubmed/32165796 http://dx.doi.org/10.4103/jgid.jgid_110_19 |
work_keys_str_mv | AT bhardwajsoniabhonchal isolationofanovelphageandtargetingbiofilmsofdrugresistantoralenterococci AT mehtamanjula isolationofanovelphageandtargetingbiofilmsofdrugresistantoralenterococci AT soodshaveta isolationofanovelphageandtargetingbiofilmsofdrugresistantoralenterococci AT sharmajyoti isolationofanovelphageandtargetingbiofilmsofdrugresistantoralenterococci |