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Genome sequencing of Aspergillus glaucus ‘CCHA’ provides insights into salt-stress adaptation

Aspergillus, as a genus of filamentous fungi, has members that display a variety of different behavioural strategies, which are affected by various environmental factors. The decoded genomic sequences of many species vary greatly in their evolutionary similarities, encouraging studies on the functio...

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Autores principales: Qiu, Wenmin, Li, Jingen, Wei, Yi, Fan, Feiyu, Jiang, Jing, Liu, Mingying, Han, Xiaojiao, Tian, Chaoguang, Zhang, Shihong, Zhuo, Renying
Formato: Online Artículo Texto
Lenguaje:English
Publicado: PeerJ Inc. 2020
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7045888/
https://www.ncbi.nlm.nih.gov/pubmed/32140304
http://dx.doi.org/10.7717/peerj.8609
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author Qiu, Wenmin
Li, Jingen
Wei, Yi
Fan, Feiyu
Jiang, Jing
Liu, Mingying
Han, Xiaojiao
Tian, Chaoguang
Zhang, Shihong
Zhuo, Renying
author_facet Qiu, Wenmin
Li, Jingen
Wei, Yi
Fan, Feiyu
Jiang, Jing
Liu, Mingying
Han, Xiaojiao
Tian, Chaoguang
Zhang, Shihong
Zhuo, Renying
author_sort Qiu, Wenmin
collection PubMed
description Aspergillus, as a genus of filamentous fungi, has members that display a variety of different behavioural strategies, which are affected by various environmental factors. The decoded genomic sequences of many species vary greatly in their evolutionary similarities, encouraging studies on the functions and evolution of the Aspergillus genome in complex natural environments. Here, we present the 26 Mb de novo assembled high-quality reference genome of Aspergillus glaucus ‘China Changchun halophilic Aspergillus’ (CCHA), which was isolated from the surface of plants growing near a salt mine in Jilin, China, based on data from whole-genome shotgun sequencing using Illumina Solexa technology. The sequence, coupled with data from comprehensive transcriptomic survey analyses, indicated that the redox state and transmembrane transport might be critical molecular mechanisms for the adaptation of A. glaucus ‘CCHA’ to the high-salt environment of the saltern. The isolation of salt tolerance-related genes, such as CCHA-2114, and their overexpression in Escherichia coli demonstrated that A. glucus ‘CCHA’ is an excellent organism for the isolation and identification of salt tolerant-related genes. These data expand our understanding of the evolution and functions of fungal and microbial genomes, and offer multiple target genes for crop salt-tolerance improvement through genetic engineering.
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spelling pubmed-70458882020-03-05 Genome sequencing of Aspergillus glaucus ‘CCHA’ provides insights into salt-stress adaptation Qiu, Wenmin Li, Jingen Wei, Yi Fan, Feiyu Jiang, Jing Liu, Mingying Han, Xiaojiao Tian, Chaoguang Zhang, Shihong Zhuo, Renying PeerJ Biotechnology Aspergillus, as a genus of filamentous fungi, has members that display a variety of different behavioural strategies, which are affected by various environmental factors. The decoded genomic sequences of many species vary greatly in their evolutionary similarities, encouraging studies on the functions and evolution of the Aspergillus genome in complex natural environments. Here, we present the 26 Mb de novo assembled high-quality reference genome of Aspergillus glaucus ‘China Changchun halophilic Aspergillus’ (CCHA), which was isolated from the surface of plants growing near a salt mine in Jilin, China, based on data from whole-genome shotgun sequencing using Illumina Solexa technology. The sequence, coupled with data from comprehensive transcriptomic survey analyses, indicated that the redox state and transmembrane transport might be critical molecular mechanisms for the adaptation of A. glaucus ‘CCHA’ to the high-salt environment of the saltern. The isolation of salt tolerance-related genes, such as CCHA-2114, and their overexpression in Escherichia coli demonstrated that A. glucus ‘CCHA’ is an excellent organism for the isolation and identification of salt tolerant-related genes. These data expand our understanding of the evolution and functions of fungal and microbial genomes, and offer multiple target genes for crop salt-tolerance improvement through genetic engineering. PeerJ Inc. 2020-02-24 /pmc/articles/PMC7045888/ /pubmed/32140304 http://dx.doi.org/10.7717/peerj.8609 Text en © 2020 Qiu et al. https://creativecommons.org/licenses/by/4.0/ This is an open access article distributed under the terms of the Creative Commons Attribution License (https://creativecommons.org/licenses/by/4.0/) , which permits unrestricted use, distribution, reproduction and adaptation in any medium and for any purpose provided that it is properly attributed. For attribution, the original author(s), title, publication source (PeerJ) and either DOI or URL of the article must be cited.
spellingShingle Biotechnology
Qiu, Wenmin
Li, Jingen
Wei, Yi
Fan, Feiyu
Jiang, Jing
Liu, Mingying
Han, Xiaojiao
Tian, Chaoguang
Zhang, Shihong
Zhuo, Renying
Genome sequencing of Aspergillus glaucus ‘CCHA’ provides insights into salt-stress adaptation
title Genome sequencing of Aspergillus glaucus ‘CCHA’ provides insights into salt-stress adaptation
title_full Genome sequencing of Aspergillus glaucus ‘CCHA’ provides insights into salt-stress adaptation
title_fullStr Genome sequencing of Aspergillus glaucus ‘CCHA’ provides insights into salt-stress adaptation
title_full_unstemmed Genome sequencing of Aspergillus glaucus ‘CCHA’ provides insights into salt-stress adaptation
title_short Genome sequencing of Aspergillus glaucus ‘CCHA’ provides insights into salt-stress adaptation
title_sort genome sequencing of aspergillus glaucus ‘ccha’ provides insights into salt-stress adaptation
topic Biotechnology
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7045888/
https://www.ncbi.nlm.nih.gov/pubmed/32140304
http://dx.doi.org/10.7717/peerj.8609
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