Generation of two tdTomato reporter induced pluripotent stem cell lines (NHLBIi003-A-1 and NHLBIi003-A-2) by AAVS1 safe harbor gene-editing

Human induced pluripotent stem cells (iPSCs) that express stable and robust fluorescent proteins have proven to be indispensable in basic and translational research. These reporter iPSC lines can greatly facilitate cell imaging, sorting, and tracking in vitro and in vivo studies. Here, we document t...

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Detalles Bibliográficos
Autores principales: Patterson, Kira, Linask, Kaari L., Beers, Jeanette, Zou, Jizhong
Formato: Online Artículo Texto
Lenguaje:English
Publicado: 2019
Materias:
Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7046126/
https://www.ncbi.nlm.nih.gov/pubmed/31869686
http://dx.doi.org/10.1016/j.scr.2019.101673
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author Patterson, Kira
Linask, Kaari L.
Beers, Jeanette
Zou, Jizhong
author_facet Patterson, Kira
Linask, Kaari L.
Beers, Jeanette
Zou, Jizhong
author_sort Patterson, Kira
collection PubMed
description Human induced pluripotent stem cells (iPSCs) that express stable and robust fluorescent proteins have proven to be indispensable in basic and translational research. These reporter iPSC lines can greatly facilitate cell imaging, sorting, and tracking in vitro and in vivo studies. Here, we document two reporter human iPSC lines generated by gene-editing technologies that precisely integrated one-copy of a tdTomato transgene driven by strong CAG promoter into the AAVS1 human safe harbor locus.
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spelling pubmed-70461262020-02-27 Generation of two tdTomato reporter induced pluripotent stem cell lines (NHLBIi003-A-1 and NHLBIi003-A-2) by AAVS1 safe harbor gene-editing Patterson, Kira Linask, Kaari L. Beers, Jeanette Zou, Jizhong Stem Cell Res Article Human induced pluripotent stem cells (iPSCs) that express stable and robust fluorescent proteins have proven to be indispensable in basic and translational research. These reporter iPSC lines can greatly facilitate cell imaging, sorting, and tracking in vitro and in vivo studies. Here, we document two reporter human iPSC lines generated by gene-editing technologies that precisely integrated one-copy of a tdTomato transgene driven by strong CAG promoter into the AAVS1 human safe harbor locus. 2019-12-06 2020-01 /pmc/articles/PMC7046126/ /pubmed/31869686 http://dx.doi.org/10.1016/j.scr.2019.101673 Text en https://creativecommons.org/licenses/by-nc-nd/4.0/This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/BY-NC-ND/4.0/ (https://creativecommons.org/licenses/by-nc-nd/4.0/) ).
spellingShingle Article
Patterson, Kira
Linask, Kaari L.
Beers, Jeanette
Zou, Jizhong
Generation of two tdTomato reporter induced pluripotent stem cell lines (NHLBIi003-A-1 and NHLBIi003-A-2) by AAVS1 safe harbor gene-editing
title Generation of two tdTomato reporter induced pluripotent stem cell lines (NHLBIi003-A-1 and NHLBIi003-A-2) by AAVS1 safe harbor gene-editing
title_full Generation of two tdTomato reporter induced pluripotent stem cell lines (NHLBIi003-A-1 and NHLBIi003-A-2) by AAVS1 safe harbor gene-editing
title_fullStr Generation of two tdTomato reporter induced pluripotent stem cell lines (NHLBIi003-A-1 and NHLBIi003-A-2) by AAVS1 safe harbor gene-editing
title_full_unstemmed Generation of two tdTomato reporter induced pluripotent stem cell lines (NHLBIi003-A-1 and NHLBIi003-A-2) by AAVS1 safe harbor gene-editing
title_short Generation of two tdTomato reporter induced pluripotent stem cell lines (NHLBIi003-A-1 and NHLBIi003-A-2) by AAVS1 safe harbor gene-editing
title_sort generation of two tdtomato reporter induced pluripotent stem cell lines (nhlbii003-a-1 and nhlbii003-a-2) by aavs1 safe harbor gene-editing
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7046126/
https://www.ncbi.nlm.nih.gov/pubmed/31869686
http://dx.doi.org/10.1016/j.scr.2019.101673
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