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Generation of human androgenetic induced pluripotent stem cells
In humans, parthenogenesis and androgenesis occur naturally in mature cystic ovarian teratomas and androgenetic complete hydatidiform moles (CHM), respectively. Our previous study has reported human parthenogenetic induced pluripotent stem cells from ovarian teratoma–derived fibroblasts and screenin...
Autores principales: | , , , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Nature Publishing Group UK
2020
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7046633/ https://www.ncbi.nlm.nih.gov/pubmed/32109236 http://dx.doi.org/10.1038/s41598-020-60363-1 |
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author | Choi, Na Young Bang, Jin Seok Park, Yo Seph Lee, Minseong Hwang, Han Sung Ko, Kisung Myung, Soon Chul Tapia, Natalia Schöler, Hans R. Kim, Gwang Jun Ko, Kinarm |
author_facet | Choi, Na Young Bang, Jin Seok Park, Yo Seph Lee, Minseong Hwang, Han Sung Ko, Kisung Myung, Soon Chul Tapia, Natalia Schöler, Hans R. Kim, Gwang Jun Ko, Kinarm |
author_sort | Choi, Na Young |
collection | PubMed |
description | In humans, parthenogenesis and androgenesis occur naturally in mature cystic ovarian teratomas and androgenetic complete hydatidiform moles (CHM), respectively. Our previous study has reported human parthenogenetic induced pluripotent stem cells from ovarian teratoma–derived fibroblasts and screening of imprinted genes using genome-wide DNA methylation analysis. However, due to the lack of the counterparts of uniparental cells, identification of new imprinted differentially methylated regions has been limited. CHM are inherited from only the paternal genome. In this study, we generated human androgenetic induced pluripotent stem cells (AgHiPSCs) from primary androgenetic fibroblasts derived from CHM. To investigate the pluripotency state of AgHiPSCs, we analyzed their cellular and molecular characteristics. We tested the DNA methylation status of imprinted genes using bisulfite sequencing and demonstrated the androgenetic identity of AgHiPSCs. AgHiPSCs might be an attractive alternative source of human androgenetic embryonic stem cells. Furthermore, AgHiPSCs can be used in regenerative medicine, for analysis of genomic imprinting, to study imprinting-related development, and for disease modeling in humans. |
format | Online Article Text |
id | pubmed-7046633 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2020 |
publisher | Nature Publishing Group UK |
record_format | MEDLINE/PubMed |
spelling | pubmed-70466332020-03-04 Generation of human androgenetic induced pluripotent stem cells Choi, Na Young Bang, Jin Seok Park, Yo Seph Lee, Minseong Hwang, Han Sung Ko, Kisung Myung, Soon Chul Tapia, Natalia Schöler, Hans R. Kim, Gwang Jun Ko, Kinarm Sci Rep Article In humans, parthenogenesis and androgenesis occur naturally in mature cystic ovarian teratomas and androgenetic complete hydatidiform moles (CHM), respectively. Our previous study has reported human parthenogenetic induced pluripotent stem cells from ovarian teratoma–derived fibroblasts and screening of imprinted genes using genome-wide DNA methylation analysis. However, due to the lack of the counterparts of uniparental cells, identification of new imprinted differentially methylated regions has been limited. CHM are inherited from only the paternal genome. In this study, we generated human androgenetic induced pluripotent stem cells (AgHiPSCs) from primary androgenetic fibroblasts derived from CHM. To investigate the pluripotency state of AgHiPSCs, we analyzed their cellular and molecular characteristics. We tested the DNA methylation status of imprinted genes using bisulfite sequencing and demonstrated the androgenetic identity of AgHiPSCs. AgHiPSCs might be an attractive alternative source of human androgenetic embryonic stem cells. Furthermore, AgHiPSCs can be used in regenerative medicine, for analysis of genomic imprinting, to study imprinting-related development, and for disease modeling in humans. Nature Publishing Group UK 2020-02-27 /pmc/articles/PMC7046633/ /pubmed/32109236 http://dx.doi.org/10.1038/s41598-020-60363-1 Text en © The Author(s) 2020 Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/. |
spellingShingle | Article Choi, Na Young Bang, Jin Seok Park, Yo Seph Lee, Minseong Hwang, Han Sung Ko, Kisung Myung, Soon Chul Tapia, Natalia Schöler, Hans R. Kim, Gwang Jun Ko, Kinarm Generation of human androgenetic induced pluripotent stem cells |
title | Generation of human androgenetic induced pluripotent stem cells |
title_full | Generation of human androgenetic induced pluripotent stem cells |
title_fullStr | Generation of human androgenetic induced pluripotent stem cells |
title_full_unstemmed | Generation of human androgenetic induced pluripotent stem cells |
title_short | Generation of human androgenetic induced pluripotent stem cells |
title_sort | generation of human androgenetic induced pluripotent stem cells |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7046633/ https://www.ncbi.nlm.nih.gov/pubmed/32109236 http://dx.doi.org/10.1038/s41598-020-60363-1 |
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