Cargando…
Speckle-tracking echocardiography combined with imaging mass spectrometry assesses region-dependent alterations
Left ventricular (LV) contraction is characterized by shortening and thickening of longitudinal and circumferential fibres. To date, it is poorly understood how LV deformation is altered in the pathogenesis of streptozotocin (STZ)-induced type 1 diabetes mellitus-associated diabetic cardiomyopathy a...
Autores principales: | , , , , , , , , , , |
---|---|
Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Nature Publishing Group UK
2020
|
Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7046677/ https://www.ncbi.nlm.nih.gov/pubmed/32108156 http://dx.doi.org/10.1038/s41598-020-60594-2 |
_version_ | 1783501997763198976 |
---|---|
author | Pappritz, Kathleen Grune, Jana Klein, Oliver Hegemann, Niklas Dong, Fengquan El-Shafeey, Muhammad Lin, Jie Kuebler, Wolfgang M. Kintscher, Ulrich Tschöpe, Carsten Van Linthout, Sophie |
author_facet | Pappritz, Kathleen Grune, Jana Klein, Oliver Hegemann, Niklas Dong, Fengquan El-Shafeey, Muhammad Lin, Jie Kuebler, Wolfgang M. Kintscher, Ulrich Tschöpe, Carsten Van Linthout, Sophie |
author_sort | Pappritz, Kathleen |
collection | PubMed |
description | Left ventricular (LV) contraction is characterized by shortening and thickening of longitudinal and circumferential fibres. To date, it is poorly understood how LV deformation is altered in the pathogenesis of streptozotocin (STZ)-induced type 1 diabetes mellitus-associated diabetic cardiomyopathy and how this is associated with changes in cardiac structural composition. To gain further insights in these LV alterations, eight-week-old C57BL6/j mice were intraperitoneally injected with 50 mg/kg body weight STZ during 5 consecutive days. Six, 9, and 12 weeks (w) post injections, echocardiographic analysis was performed using a Vevo 3100 device coupled to a 30-MHz linear-frequency transducer. Speckle-tracking echocardiography (STE) demonstrated impaired global longitudinal peak strain (GLS) in STZ versus control mice at all time points. 9w STZ animals displayed an impaired global circumferential peak strain (GCS) versus 6w and 12w STZ mice. They further exhibited decreased myocardial deformation behaviour of the anterior and posterior base versus controls, which was paralleled with an elevated collagen I/III protein ratio. Additionally, hypothesis-free proteome analysis by imaging mass spectrometry (IMS) identified regional- and time-dependent changes of proteins affecting sarcomere mechanics between STZ and control mice. In conclusion, STZ-induced diabetic cardiomyopathy changes global cardiac deformation associated with alterations in cardiac sarcomere proteins. |
format | Online Article Text |
id | pubmed-7046677 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2020 |
publisher | Nature Publishing Group UK |
record_format | MEDLINE/PubMed |
spelling | pubmed-70466772020-03-05 Speckle-tracking echocardiography combined with imaging mass spectrometry assesses region-dependent alterations Pappritz, Kathleen Grune, Jana Klein, Oliver Hegemann, Niklas Dong, Fengquan El-Shafeey, Muhammad Lin, Jie Kuebler, Wolfgang M. Kintscher, Ulrich Tschöpe, Carsten Van Linthout, Sophie Sci Rep Article Left ventricular (LV) contraction is characterized by shortening and thickening of longitudinal and circumferential fibres. To date, it is poorly understood how LV deformation is altered in the pathogenesis of streptozotocin (STZ)-induced type 1 diabetes mellitus-associated diabetic cardiomyopathy and how this is associated with changes in cardiac structural composition. To gain further insights in these LV alterations, eight-week-old C57BL6/j mice were intraperitoneally injected with 50 mg/kg body weight STZ during 5 consecutive days. Six, 9, and 12 weeks (w) post injections, echocardiographic analysis was performed using a Vevo 3100 device coupled to a 30-MHz linear-frequency transducer. Speckle-tracking echocardiography (STE) demonstrated impaired global longitudinal peak strain (GLS) in STZ versus control mice at all time points. 9w STZ animals displayed an impaired global circumferential peak strain (GCS) versus 6w and 12w STZ mice. They further exhibited decreased myocardial deformation behaviour of the anterior and posterior base versus controls, which was paralleled with an elevated collagen I/III protein ratio. Additionally, hypothesis-free proteome analysis by imaging mass spectrometry (IMS) identified regional- and time-dependent changes of proteins affecting sarcomere mechanics between STZ and control mice. In conclusion, STZ-induced diabetic cardiomyopathy changes global cardiac deformation associated with alterations in cardiac sarcomere proteins. Nature Publishing Group UK 2020-02-27 /pmc/articles/PMC7046677/ /pubmed/32108156 http://dx.doi.org/10.1038/s41598-020-60594-2 Text en © The Author(s) 2020 Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/. |
spellingShingle | Article Pappritz, Kathleen Grune, Jana Klein, Oliver Hegemann, Niklas Dong, Fengquan El-Shafeey, Muhammad Lin, Jie Kuebler, Wolfgang M. Kintscher, Ulrich Tschöpe, Carsten Van Linthout, Sophie Speckle-tracking echocardiography combined with imaging mass spectrometry assesses region-dependent alterations |
title | Speckle-tracking echocardiography combined with imaging mass spectrometry assesses region-dependent alterations |
title_full | Speckle-tracking echocardiography combined with imaging mass spectrometry assesses region-dependent alterations |
title_fullStr | Speckle-tracking echocardiography combined with imaging mass spectrometry assesses region-dependent alterations |
title_full_unstemmed | Speckle-tracking echocardiography combined with imaging mass spectrometry assesses region-dependent alterations |
title_short | Speckle-tracking echocardiography combined with imaging mass spectrometry assesses region-dependent alterations |
title_sort | speckle-tracking echocardiography combined with imaging mass spectrometry assesses region-dependent alterations |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7046677/ https://www.ncbi.nlm.nih.gov/pubmed/32108156 http://dx.doi.org/10.1038/s41598-020-60594-2 |
work_keys_str_mv | AT pappritzkathleen speckletrackingechocardiographycombinedwithimagingmassspectrometryassessesregiondependentalterations AT grunejana speckletrackingechocardiographycombinedwithimagingmassspectrometryassessesregiondependentalterations AT kleinoliver speckletrackingechocardiographycombinedwithimagingmassspectrometryassessesregiondependentalterations AT hegemannniklas speckletrackingechocardiographycombinedwithimagingmassspectrometryassessesregiondependentalterations AT dongfengquan speckletrackingechocardiographycombinedwithimagingmassspectrometryassessesregiondependentalterations AT elshafeeymuhammad speckletrackingechocardiographycombinedwithimagingmassspectrometryassessesregiondependentalterations AT linjie speckletrackingechocardiographycombinedwithimagingmassspectrometryassessesregiondependentalterations AT kueblerwolfgangm speckletrackingechocardiographycombinedwithimagingmassspectrometryassessesregiondependentalterations AT kintscherulrich speckletrackingechocardiographycombinedwithimagingmassspectrometryassessesregiondependentalterations AT tschopecarsten speckletrackingechocardiographycombinedwithimagingmassspectrometryassessesregiondependentalterations AT vanlinthoutsophie speckletrackingechocardiographycombinedwithimagingmassspectrometryassessesregiondependentalterations |