A protocol for metabolic characterization of human induced pluripotent stem cell-derived cardiomyocytes (iPS-CM)

Recent advances in human induced pluripotent stem cell-derived cardiomyocytes (iPSCM) field offer a novel platform for modeling cardiac metabolism, heart diseases drug candidates screening and cardiac toxicity assessments. These workflows require a fully functional characterization of iPSCMs. Here w...

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Detalles Bibliográficos
Autores principales: House, Alisha, Fatica, Erica, Shah, Rohan, Stergar, Jared, Pearce, Ryan, Sandlers, Yana
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Elsevier 2019
Materias:
Biochemistry, Genetics and Molecular Biology
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7046813/
https://www.ncbi.nlm.nih.gov/pubmed/32140435
http://dx.doi.org/10.1016/j.mex.2019.05.028
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author House, Alisha
Fatica, Erica
Shah, Rohan
Stergar, Jared
Pearce, Ryan
Sandlers, Yana
author_facet House, Alisha
Fatica, Erica
Shah, Rohan
Stergar, Jared
Pearce, Ryan
Sandlers, Yana
author_sort House, Alisha
collection PubMed
description Recent advances in human induced pluripotent stem cell-derived cardiomyocytes (iPSCM) field offer a novel platform for modeling cardiac metabolism, heart diseases drug candidates screening and cardiac toxicity assessments. These workflows require a fully functional characterization of iPSCMs. Here we report a step by step protocol for iPSCM metabolic characterization. The described assays cover analysis of small metabolites involved in a vital metabolic pathways.
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spelling pubmed-70468132020-03-05 A protocol for metabolic characterization of human induced pluripotent stem cell-derived cardiomyocytes (iPS-CM) House, Alisha Fatica, Erica Shah, Rohan Stergar, Jared Pearce, Ryan Sandlers, Yana MethodsX Biochemistry, Genetics and Molecular Biology Recent advances in human induced pluripotent stem cell-derived cardiomyocytes (iPSCM) field offer a novel platform for modeling cardiac metabolism, heart diseases drug candidates screening and cardiac toxicity assessments. These workflows require a fully functional characterization of iPSCMs. Here we report a step by step protocol for iPSCM metabolic characterization. The described assays cover analysis of small metabolites involved in a vital metabolic pathways. Elsevier 2019-05-29 /pmc/articles/PMC7046813/ /pubmed/32140435 http://dx.doi.org/10.1016/j.mex.2019.05.028 Text en © 2019 The Authors http://creativecommons.org/licenses/by/4.0/ This is an open access article under the CC BY license (http://creativecommons.org/licenses/by/4.0/).
spellingShingle Biochemistry, Genetics and Molecular Biology
House, Alisha
Fatica, Erica
Shah, Rohan
Stergar, Jared
Pearce, Ryan
Sandlers, Yana
A protocol for metabolic characterization of human induced pluripotent stem cell-derived cardiomyocytes (iPS-CM)
title A protocol for metabolic characterization of human induced pluripotent stem cell-derived cardiomyocytes (iPS-CM)
title_full A protocol for metabolic characterization of human induced pluripotent stem cell-derived cardiomyocytes (iPS-CM)
title_fullStr A protocol for metabolic characterization of human induced pluripotent stem cell-derived cardiomyocytes (iPS-CM)
title_full_unstemmed A protocol for metabolic characterization of human induced pluripotent stem cell-derived cardiomyocytes (iPS-CM)
title_short A protocol for metabolic characterization of human induced pluripotent stem cell-derived cardiomyocytes (iPS-CM)
title_sort protocol for metabolic characterization of human induced pluripotent stem cell-derived cardiomyocytes (ips-cm)
topic Biochemistry, Genetics and Molecular Biology
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7046813/
https://www.ncbi.nlm.nih.gov/pubmed/32140435
http://dx.doi.org/10.1016/j.mex.2019.05.028
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