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Identification of Core Gene Expression Signature and Key Pathways in Colorectal Cancer
OBJECTIVE: Colorectal cancer (CRC) is considered the most prevalent malignant tumor that contributes to high cancer-related mortality. However, the signaling pathways involved in CRC and CRC-driven genes are largely unknown. We sought to discover a novel biomarker in CRC. MATERIALS AND METHODS: All...
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Formato: | Online Artículo Texto |
Lenguaje: | English |
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Frontiers Media S.A.
2020
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Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7046836/ https://www.ncbi.nlm.nih.gov/pubmed/32153633 http://dx.doi.org/10.3389/fgene.2020.00045 |
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author | Ding, Xiang Duan, Houyu Luo, Hesheng |
author_facet | Ding, Xiang Duan, Houyu Luo, Hesheng |
author_sort | Ding, Xiang |
collection | PubMed |
description | OBJECTIVE: Colorectal cancer (CRC) is considered the most prevalent malignant tumor that contributes to high cancer-related mortality. However, the signaling pathways involved in CRC and CRC-driven genes are largely unknown. We sought to discover a novel biomarker in CRC. MATERIALS AND METHODS: All clinical CRC samples (n = 20) were from Renmin Hospital of Wuhan University. We first selected MAD2L1 by integrated bioinformatics analysis of a GSE dataset. Next, the expression of MAD2L1 in tissues and cell lines was verified by quantitative real-time PCR. The effects of MAD2L1 on cell growth, proliferation, the cell cycle, and apoptosis were examined by in vitro assays. RESULTS: We identified 683 shared DEGs (420 upregulated and 263 downregulated), and the top twenty genes (CDK1, CCNA2, TOP2A, PLK1, MAD2L1, AURKA, BUB1B, UBE2C, TPX2, RRM2, KIF11, NCAPG, MELK, NUSAP1, MCM4, RFC4, PTTG1, CHEK1, CEP55, DTL) were selected by integrated analysis. These hub genes were significantly overexpressed in CRC samples and were positively correlated. Our data revealed that the expression of MAD2L1 in CRC tissues is higher than that in normal tissues. MAD2L1 knockdown significantly suppressed CRC cell growth by impairing cell cycle progression and inducing cell apoptosis. CONCLUSION: MAD2L1, as a novel oncogenic gene, plays a role in regulating cancer cell growth and apoptosis and could be used as a new biomarker for diagnosis and therapy in CRC. |
format | Online Article Text |
id | pubmed-7046836 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2020 |
publisher | Frontiers Media S.A. |
record_format | MEDLINE/PubMed |
spelling | pubmed-70468362020-03-09 Identification of Core Gene Expression Signature and Key Pathways in Colorectal Cancer Ding, Xiang Duan, Houyu Luo, Hesheng Front Genet Genetics OBJECTIVE: Colorectal cancer (CRC) is considered the most prevalent malignant tumor that contributes to high cancer-related mortality. However, the signaling pathways involved in CRC and CRC-driven genes are largely unknown. We sought to discover a novel biomarker in CRC. MATERIALS AND METHODS: All clinical CRC samples (n = 20) were from Renmin Hospital of Wuhan University. We first selected MAD2L1 by integrated bioinformatics analysis of a GSE dataset. Next, the expression of MAD2L1 in tissues and cell lines was verified by quantitative real-time PCR. The effects of MAD2L1 on cell growth, proliferation, the cell cycle, and apoptosis were examined by in vitro assays. RESULTS: We identified 683 shared DEGs (420 upregulated and 263 downregulated), and the top twenty genes (CDK1, CCNA2, TOP2A, PLK1, MAD2L1, AURKA, BUB1B, UBE2C, TPX2, RRM2, KIF11, NCAPG, MELK, NUSAP1, MCM4, RFC4, PTTG1, CHEK1, CEP55, DTL) were selected by integrated analysis. These hub genes were significantly overexpressed in CRC samples and were positively correlated. Our data revealed that the expression of MAD2L1 in CRC tissues is higher than that in normal tissues. MAD2L1 knockdown significantly suppressed CRC cell growth by impairing cell cycle progression and inducing cell apoptosis. CONCLUSION: MAD2L1, as a novel oncogenic gene, plays a role in regulating cancer cell growth and apoptosis and could be used as a new biomarker for diagnosis and therapy in CRC. Frontiers Media S.A. 2020-02-21 /pmc/articles/PMC7046836/ /pubmed/32153633 http://dx.doi.org/10.3389/fgene.2020.00045 Text en Copyright © 2020 Ding, Duan and Luo http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms. |
spellingShingle | Genetics Ding, Xiang Duan, Houyu Luo, Hesheng Identification of Core Gene Expression Signature and Key Pathways in Colorectal Cancer |
title | Identification of Core Gene Expression Signature and Key Pathways in Colorectal Cancer |
title_full | Identification of Core Gene Expression Signature and Key Pathways in Colorectal Cancer |
title_fullStr | Identification of Core Gene Expression Signature and Key Pathways in Colorectal Cancer |
title_full_unstemmed | Identification of Core Gene Expression Signature and Key Pathways in Colorectal Cancer |
title_short | Identification of Core Gene Expression Signature and Key Pathways in Colorectal Cancer |
title_sort | identification of core gene expression signature and key pathways in colorectal cancer |
topic | Genetics |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7046836/ https://www.ncbi.nlm.nih.gov/pubmed/32153633 http://dx.doi.org/10.3389/fgene.2020.00045 |
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