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Validation and Characterization of a Seed Number Per Silique Quantitative Trait Locus qSN.A7 in Rapeseed (Brassica napus L.)

Seed number is a key character/trait tightly related to the plant fitness/evolution and crop domestication/improvement. The seed number per silique (SNPS) shows a huge variation from several to more than 30, however the underlying regulatory mechanisms are poorly known, which has hindered its improv...

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Autores principales: Zhu, Yaoyao, Ye, Jiang, Zhan, Jiepeng, Zheng, Xiaoxiao, Zhang, Jiangjiang, Shi, Jiaqin, Wang, Xinfa, Liu, Guihua, Wang, Hanzhong
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Frontiers Media S.A. 2020
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7047150/
https://www.ncbi.nlm.nih.gov/pubmed/32153604
http://dx.doi.org/10.3389/fpls.2020.00068
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author Zhu, Yaoyao
Ye, Jiang
Zhan, Jiepeng
Zheng, Xiaoxiao
Zhang, Jiangjiang
Shi, Jiaqin
Wang, Xinfa
Liu, Guihua
Wang, Hanzhong
author_facet Zhu, Yaoyao
Ye, Jiang
Zhan, Jiepeng
Zheng, Xiaoxiao
Zhang, Jiangjiang
Shi, Jiaqin
Wang, Xinfa
Liu, Guihua
Wang, Hanzhong
author_sort Zhu, Yaoyao
collection PubMed
description Seed number is a key character/trait tightly related to the plant fitness/evolution and crop domestication/improvement. The seed number per silique (SNPS) shows a huge variation from several to more than 30, however the underlying regulatory mechanisms are poorly known, which has hindered its improvement. To answer this question, several representative lines with extreme SNPS were previously subjected to systematic genetic and cytological analyses. The results showed that the natural variation of seed number per silique is mainly controlled by maternal and embryonic genotype, which are co-determined by ovule number per ovary, fertile ovule ratio, ovule fertilization rate, and fertilized ovule development rate. More importantly, we also mapped two repeatable quantitative trait loci (QTLs) for SNPS using the F(2:3) population derived from Zhongshuang11 and No. 73290, of which the major QTL qSN.A6 has been fine-mapped. In the current study, the near-isogenic lines (NILs) of qSN.A7 were successfully developed by the successive backcross of F(1) with Zhongshuang11. First, the effect of qSN.A7 was validated by evaluating the SNPS of two types of homozygous NILs from BC(3)F(2) population, which showed a significant difference of 2.23 on average. Then, qSN.A7 was successfully fine-mapped from the original 4.237 to 1.389 Mb, using a BC(4)F(2) segregating population of 2,551 individuals. To further clarify the regulatory mechanism of qSN.A7, the two types of homologous NILs were subjected to genetic and cytological analyses. The results showed that the difference in SNPS between the two homologous NILs was determined by the embryonic genotypic effect. Highly accordant with this, no significant difference was observed in ovule number per ovary, ovule fertility, fertilization rate, and pollen fertility between the two homologous NILs. Therefore, the regulatory mechanism of qSN.A7 is completely different from the cloned qSS.C9 and qSN.A6. These results will advance the understanding of SNPS and facilitate gene cloning and molecular breeding in Brassica napus.
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spelling pubmed-70471502020-03-09 Validation and Characterization of a Seed Number Per Silique Quantitative Trait Locus qSN.A7 in Rapeseed (Brassica napus L.) Zhu, Yaoyao Ye, Jiang Zhan, Jiepeng Zheng, Xiaoxiao Zhang, Jiangjiang Shi, Jiaqin Wang, Xinfa Liu, Guihua Wang, Hanzhong Front Plant Sci Plant Science Seed number is a key character/trait tightly related to the plant fitness/evolution and crop domestication/improvement. The seed number per silique (SNPS) shows a huge variation from several to more than 30, however the underlying regulatory mechanisms are poorly known, which has hindered its improvement. To answer this question, several representative lines with extreme SNPS were previously subjected to systematic genetic and cytological analyses. The results showed that the natural variation of seed number per silique is mainly controlled by maternal and embryonic genotype, which are co-determined by ovule number per ovary, fertile ovule ratio, ovule fertilization rate, and fertilized ovule development rate. More importantly, we also mapped two repeatable quantitative trait loci (QTLs) for SNPS using the F(2:3) population derived from Zhongshuang11 and No. 73290, of which the major QTL qSN.A6 has been fine-mapped. In the current study, the near-isogenic lines (NILs) of qSN.A7 were successfully developed by the successive backcross of F(1) with Zhongshuang11. First, the effect of qSN.A7 was validated by evaluating the SNPS of two types of homozygous NILs from BC(3)F(2) population, which showed a significant difference of 2.23 on average. Then, qSN.A7 was successfully fine-mapped from the original 4.237 to 1.389 Mb, using a BC(4)F(2) segregating population of 2,551 individuals. To further clarify the regulatory mechanism of qSN.A7, the two types of homologous NILs were subjected to genetic and cytological analyses. The results showed that the difference in SNPS between the two homologous NILs was determined by the embryonic genotypic effect. Highly accordant with this, no significant difference was observed in ovule number per ovary, ovule fertility, fertilization rate, and pollen fertility between the two homologous NILs. Therefore, the regulatory mechanism of qSN.A7 is completely different from the cloned qSS.C9 and qSN.A6. These results will advance the understanding of SNPS and facilitate gene cloning and molecular breeding in Brassica napus. Frontiers Media S.A. 2020-02-21 /pmc/articles/PMC7047150/ /pubmed/32153604 http://dx.doi.org/10.3389/fpls.2020.00068 Text en Copyright © 2020 Zhu, Ye, Zhan, Zheng, Zhang, Shi, Wang, Liu and Wang http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
spellingShingle Plant Science
Zhu, Yaoyao
Ye, Jiang
Zhan, Jiepeng
Zheng, Xiaoxiao
Zhang, Jiangjiang
Shi, Jiaqin
Wang, Xinfa
Liu, Guihua
Wang, Hanzhong
Validation and Characterization of a Seed Number Per Silique Quantitative Trait Locus qSN.A7 in Rapeseed (Brassica napus L.)
title Validation and Characterization of a Seed Number Per Silique Quantitative Trait Locus qSN.A7 in Rapeseed (Brassica napus L.)
title_full Validation and Characterization of a Seed Number Per Silique Quantitative Trait Locus qSN.A7 in Rapeseed (Brassica napus L.)
title_fullStr Validation and Characterization of a Seed Number Per Silique Quantitative Trait Locus qSN.A7 in Rapeseed (Brassica napus L.)
title_full_unstemmed Validation and Characterization of a Seed Number Per Silique Quantitative Trait Locus qSN.A7 in Rapeseed (Brassica napus L.)
title_short Validation and Characterization of a Seed Number Per Silique Quantitative Trait Locus qSN.A7 in Rapeseed (Brassica napus L.)
title_sort validation and characterization of a seed number per silique quantitative trait locus qsn.a7 in rapeseed (brassica napus l.)
topic Plant Science
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7047150/
https://www.ncbi.nlm.nih.gov/pubmed/32153604
http://dx.doi.org/10.3389/fpls.2020.00068
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