Cargando…

Sedimentation Yields Long-Term Stable Protein Samples as Shown by Solid-State NMR

Today, the sedimentation of proteins into a magic-angle spinning (MAS) rotor gives access to fast and reliable sample preparation for solid-state Nuclear Magnetic Resonance (NMR), and this has allowed for the investigation of a variety of non-crystalline protein samples. High protein concentrations...

Descripción completa

Detalles Bibliográficos
Autores principales:	Wiegand, Thomas, Lacabanne, Denis, Torosyan, Anahit, Boudet, Julien, Cadalbert, Riccardo, Allain, Frédéric H.-T., Meier, Beat H., Böckmann, Anja
Formato:	Online Artículo Texto
Lenguaje:	English
Publicado:	Frontiers Media S.A. 2020
Materias:	Molecular Biosciences
Acceso en línea:	https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7047159/ https://www.ncbi.nlm.nih.gov/pubmed/32154263 http://dx.doi.org/10.3389/fmolb.2020.00017

Ejemplares similares

Including Protons in Solid-State NMR Resonance Assignment and Secondary Structure Analysis: The Example of RNA Polymerase II Subunits Rpo4/7
por: Torosyan, Anahit, et al.
Publicado: (2019)

Asparagine and Glutamine Side-Chains and Ladders in HET-s(218–289) Amyloid Fibrils Studied by Fast Magic-Angle Spinning NMR
por: Wiegand, Thomas, et al.
Publicado: (2020)

Protein Side-Chain–DNA Contacts Probed by Fast Magic-Angle Spinning NMR
por: Lacabanne, Denis, et al.
Publicado: (2020)

Correction of field instabilities in biomolecular solid-state NMR by simultaneous acquisition of a frequency reference
por: Římal, Václav, et al.
Publicado: (2022)

Efficient and stable reconstitution of the ABC transporter BmrA for solid-state NMR studies
por: Kunert, Britta, et al.
Publicado: (2014)

The conformational changes coupling ATP hydrolysis and translocation in a bacterial DnaB helicase
por: Wiegand, Thomas, et al.
Publicado: (2019)

Paramagnetic Solid‐State NMR to Localize the Metal‐Ion Cofactor in an Oligomeric DnaB Helicase
por: Zehnder, Johannes, et al.
Publicado: (2021)

Protein NMR Spectroscopy at 150 kHz Magic‐Angle Spinning Continues To Improve Resolution and Mass Sensitivity
por: Schledorn, Maarten, et al.
Publicado: (2020)

Temperature-Dependent Solid-State NMR Proton Chemical-Shift Values and Hydrogen Bonding
por: Malär, Alexander A., et al.
Publicado: (2021)

ATP Analogues for Structural Investigations: Case Studies of a DnaB Helicase and an ABC Transporter
por: Lacabanne, Denis, et al.
Publicado: (2020)

Nucleotide Binding Modes in a Motor Protein Revealed by (31)P‐ and (1)H‐Detected MAS Solid‐State NMR Spectroscopy
por: Wiegand, Thomas, et al.
Publicado: (2019)

Solid-State NMR Reveals Asymmetric ATP Hydrolysis in the Multidrug ABC Transporter BmrA
por: Lacabanne, Denis, et al.
Publicado: (2022)

Biomolecular solid-state NMR spectroscopy at 1200 MHz: the gain in resolution
por: Callon, Morgane, et al.
Publicado: (2021)

100 kHz MAS Proton-Detected NMR Spectroscopy of Hepatitis B Virus Capsids
por: Lecoq, Lauriane, et al.
Publicado: (2019)

Experimental Characterization of the Hepatitis B Virus Capsid Dynamics by Solid-State NMR
por: Malär, Alexander A., et al.
Publicado: (2022)

Alternative salt bridge formation in Aβ—a hallmark of early-onset Alzheimer's disease?
por: Schledorn, Maarten, et al.
Publicado: (2015)

Combining Cell-Free Protein Synthesis and NMR Into a Tool to Study Capsid Assembly Modulation
por: Wang, Shishan, et al.
Publicado: (2019)

Hommage to Richard R. Ernst
por: Böckmann, Anja, et al.
Publicado: (2021)

Prion Amyloid Polymorphs – The Tag Might Change It All
por: Bousset, Luc, et al.
Publicado: (2020)

Flexible-to-rigid transition is central for substrate transport in the ABC transporter BmrA from Bacillus subtilis
por: Lacabanne, Denis, et al.
Publicado: (2019)

Solid-State NMR for Studying the Structure and Dynamics of Viral Assemblies
por: Lecoq, Lauriane, et al.
Publicado: (2020)

High and fast: NMR protein–proton side-chain assignments at 160 kHz and 1.2 GHz
por: Callon, Morgane, et al.
Publicado: (2023)

Editorial: Structural dynamics of membrane proteins, Volume II
por: Raghuraman, H., et al.
Publicado: (2022)

Unlike Twins: An NMR Comparison of Two α-Synuclein Polymorphs Featuring Different Toxicity
por: Gath, Julia, et al.
Publicado: (2014)

Spinning faster: protein NMR at MAS frequencies up to 126 kHz
por: Penzel, Susanne, et al.
Publicado: (2019)

Atomic-Resolution Three-Dimensional Structure of Amyloid β Fibrils Bearing the Osaka Mutation**
por: Schütz, Anne K, et al.
Publicado: (2015)

Spectroscopic glimpses of the transition state of ATP hydrolysis trapped in a bacterial DnaB helicase
por: Malär, Alexander A., et al.
Publicado: (2021)

Reassessment of MxiH subunit orientation and fold within native Shigella T3SS needles using surface labelling and solid-state NMR
por: Verasdonck, Joeri, et al.
Publicado: (2015)

Continuous in vivo Metabolism by NMR
por: Judge, Michael T., et al.
Publicado: (2019)

Residual dipolar line width in magic-angle spinning proton solid-state NMR
por: Chávez, Matías, et al.
Publicado: (2021)

Proton‐Detected Solid‐State NMR of the Cell‐Free Synthesized α‐Helical Transmembrane Protein NS4B from Hepatitis C Virus
por: Jirasko, Vlastimil, et al.
Publicado: (2020)

Easy Synthesis of Complex Biomolecular Assemblies: Wheat Germ Cell-Free Protein Expression in Structural Biology
por: Fogeron, Marie-Laure, et al.
Publicado: (2021)

NMR Studies of Tau Protein in Tauopathies
por: Kitoka, Kristine, et al.
Publicado: (2021)

Studying Metabolism by NMR-Based Metabolomics
por: Moco, Sofia
Publicado: (2022)

Molecular elucidation of drug-induced abnormal assemblies of the hepatitis B virus capsid protein by solid-state NMR
por: Lecoq, Lauriane, et al.
Publicado: (2023)

NMR Metabolite Profiles of the Bivalve Mollusc Mytilus galloprovincialis Before and After Immune Stimulation With Vibrio splendidus
por: Frizzo, Riccardo, et al.
Publicado: (2021)

NMR Characterization of the Interactions Between Glycosaminoglycans and Proteins
por: Bu, Changkai, et al.
Publicado: (2021)

Multiplatform Metabolomics Studies of Human Cancers With NMR and Mass Spectrometry Imaging
por: Zhong, Anya B., et al.
Publicado: (2022)

The Use of NMR to Study Transient Carbohydrate—Protein Interactions
por: Nieto, Pedro M.
Publicado: (2018)

NMR Methods for Structural Characterization of Protein-Protein Complexes
por: Purslow, Jeffrey A., et al.
Publicado: (2020)

Cannot write session to /tmp/vufind_sessions/sess_rdm001gcnct2rdjep9342r5a3u