Metagenomic sequencing with spiked primer enrichment for viral diagnostics and genomic surveillance

Metagenomic next-generation sequencing (mNGS), the shotgun sequencing of RNA and DNA from clinical samples, has proved useful for broad-spectrum pathogen detection and the genomic surveillance of viral outbreaks. An additional target enrichment step is generally needed for high-sensitivity pathogen...

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Detalles Bibliográficos
Autores principales: Deng, Xianding, Achari, Asmeeta, Federman, Scot, Yu, Guixia, Somasekar, Sneha, Bártolo, Inês, Yagi, Shigeo, Mbala-Kingebeni, Placide, Kapetshi, Jimmy, Ahuka-Mundeke, Steve, Muyembe-Tamfum, Jean-Jacques, Ahmed, Asim A., Ganesh, Vijay, Tamhankar, Manasi, Patterson, Jean L., Ndembi, Nicaise, Mbanya, Dora, Kaptue, Lazare, McArthur, Carole, Muñoz-Medina, José E., Gonzalez-Bonilla, Cesar R., López, Susana, Arias, Carlos F., Arevalo, Shaun, Miller, Steve, Stone, Mars, Busch, Michael, Hsieh, Kristina, Messenger, Sharon, Wadford, Debra A., Rodgers, Mary, Cloherty, Gavin, Faria, Nuno R., Thézé, Julien, Pybus, Oliver G., Neto, Zoraima, Morais, Joana, Taveira, Nuno, R. Hackett, John, Chiu, Charles Y.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Nature Publishing Group UK 2020
Materias:
Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7047537/
https://www.ncbi.nlm.nih.gov/pubmed/31932713
http://dx.doi.org/10.1038/s41564-019-0637-9

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