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Circular RNA-0001283 Suppresses Breast Cancer Proliferation and Invasion via MiR-187/HIPK3 Axis

BACKGROUND: Circular RNAs (circRNAs) are key regulators that take part in the carcinogenesis and development of breast cancer. The current study aimed to identify the expression of and explored the function of circRNA-0001283 in breast cancer. MATERIAL/METHODS: Breast cancer tissue samples were test...

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Autores principales: Hu, Ying, Guo, Feixiang, Zhu, Hongquan, Tan, Xiaobin, Zhu, Xiansen, Liu, Xiaofeng, Zhang, Wei, Yang, Qiong, Jiang, Yaling
Formato: Online Artículo Texto
Lenguaje:English
Publicado: International Scientific Literature, Inc. 2020
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7047918/
https://www.ncbi.nlm.nih.gov/pubmed/32066649
http://dx.doi.org/10.12659/MSM.921502
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author Hu, Ying
Guo, Feixiang
Zhu, Hongquan
Tan, Xiaobin
Zhu, Xiansen
Liu, Xiaofeng
Zhang, Wei
Yang, Qiong
Jiang, Yaling
author_facet Hu, Ying
Guo, Feixiang
Zhu, Hongquan
Tan, Xiaobin
Zhu, Xiansen
Liu, Xiaofeng
Zhang, Wei
Yang, Qiong
Jiang, Yaling
author_sort Hu, Ying
collection PubMed
description BACKGROUND: Circular RNAs (circRNAs) are key regulators that take part in the carcinogenesis and development of breast cancer. The current study aimed to identify the expression of and explored the function of circRNA-0001283 in breast cancer. MATERIAL/METHODS: Breast cancer tissue samples were tested using high-throughput sequencing to identify the levels of relative genes; and proteins were addressed by using quantitative real-time polymerase chain reaction (qRT-PCR) and western-blot. Cell ability and cell apoptosis were investigated by Cell Counting Kit-8 (CCK-8) and flow cytometry. Invasion was detected by Transwell invasion assay. The identification of target genes was analyzed by dual-luciferase reporter assay. RESULT: Downregulation of circRNA-0001283 expression was observed in breast cancer tissue samples. Ectopic expression of circRNA-0001283 remarkably suppressed cell viability and invasion, and induced apoptosis in breast cancer cells. Furthermore, circRNA-0001283 bound to miR-187 and decreased the expression of miR-187, which resulted in inhibition in cell growth and invasion. Finally, we showed that circRNA-0001283 positively regulated HIPK3 expression by sponging miR-187. CONCLUSIONS: The results reveal a new functional circRNA-0001283 in breast cancer and may provide targets for developing novel therapeutic strategies for breast cancer.
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spelling pubmed-70479182020-03-12 Circular RNA-0001283 Suppresses Breast Cancer Proliferation and Invasion via MiR-187/HIPK3 Axis Hu, Ying Guo, Feixiang Zhu, Hongquan Tan, Xiaobin Zhu, Xiansen Liu, Xiaofeng Zhang, Wei Yang, Qiong Jiang, Yaling Med Sci Monit Lab/In Vitro Research BACKGROUND: Circular RNAs (circRNAs) are key regulators that take part in the carcinogenesis and development of breast cancer. The current study aimed to identify the expression of and explored the function of circRNA-0001283 in breast cancer. MATERIAL/METHODS: Breast cancer tissue samples were tested using high-throughput sequencing to identify the levels of relative genes; and proteins were addressed by using quantitative real-time polymerase chain reaction (qRT-PCR) and western-blot. Cell ability and cell apoptosis were investigated by Cell Counting Kit-8 (CCK-8) and flow cytometry. Invasion was detected by Transwell invasion assay. The identification of target genes was analyzed by dual-luciferase reporter assay. RESULT: Downregulation of circRNA-0001283 expression was observed in breast cancer tissue samples. Ectopic expression of circRNA-0001283 remarkably suppressed cell viability and invasion, and induced apoptosis in breast cancer cells. Furthermore, circRNA-0001283 bound to miR-187 and decreased the expression of miR-187, which resulted in inhibition in cell growth and invasion. Finally, we showed that circRNA-0001283 positively regulated HIPK3 expression by sponging miR-187. CONCLUSIONS: The results reveal a new functional circRNA-0001283 in breast cancer and may provide targets for developing novel therapeutic strategies for breast cancer. International Scientific Literature, Inc. 2020-02-18 /pmc/articles/PMC7047918/ /pubmed/32066649 http://dx.doi.org/10.12659/MSM.921502 Text en © Med Sci Monit, 2020 This work is licensed under Creative Common Attribution-NonCommercial-NoDerivatives 4.0 International (CC BY-NC-ND 4.0 (https://creativecommons.org/licenses/by-nc-nd/4.0/) )
spellingShingle Lab/In Vitro Research
Hu, Ying
Guo, Feixiang
Zhu, Hongquan
Tan, Xiaobin
Zhu, Xiansen
Liu, Xiaofeng
Zhang, Wei
Yang, Qiong
Jiang, Yaling
Circular RNA-0001283 Suppresses Breast Cancer Proliferation and Invasion via MiR-187/HIPK3 Axis
title Circular RNA-0001283 Suppresses Breast Cancer Proliferation and Invasion via MiR-187/HIPK3 Axis
title_full Circular RNA-0001283 Suppresses Breast Cancer Proliferation and Invasion via MiR-187/HIPK3 Axis
title_fullStr Circular RNA-0001283 Suppresses Breast Cancer Proliferation and Invasion via MiR-187/HIPK3 Axis
title_full_unstemmed Circular RNA-0001283 Suppresses Breast Cancer Proliferation and Invasion via MiR-187/HIPK3 Axis
title_short Circular RNA-0001283 Suppresses Breast Cancer Proliferation and Invasion via MiR-187/HIPK3 Axis
title_sort circular rna-0001283 suppresses breast cancer proliferation and invasion via mir-187/hipk3 axis
topic Lab/In Vitro Research
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7047918/
https://www.ncbi.nlm.nih.gov/pubmed/32066649
http://dx.doi.org/10.12659/MSM.921502
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