Purification, Structural Characterization, and Biological Activity of Polysaccharides from Lentinus velutinus

A polysaccharide (LVP) was purified from fruiting body of Lentinus velutinus by ethanol precipitation fractionation and DEAE and Sephadex G-100 column chromatography. The yield of purified polysaccharide was 0.025%. Molecular characteristics of LVP were determined by gel permeation chromatography, F...

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Detalles Bibliográficos
Autores principales: Udchumpisai, Wascharin, Bangyeekhun, Eakaphun
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Taylor & Francis 2019
Materias:
Research Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7048199/
https://www.ncbi.nlm.nih.gov/pubmed/32158606
http://dx.doi.org/10.1080/12298093.2019.1693482
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author Udchumpisai, Wascharin
Bangyeekhun, Eakaphun
author_facet Udchumpisai, Wascharin
Bangyeekhun, Eakaphun
author_sort Udchumpisai, Wascharin
collection PubMed
description A polysaccharide (LVP) was purified from fruiting body of Lentinus velutinus by ethanol precipitation fractionation and DEAE and Sephadex G-100 column chromatography. The yield of purified polysaccharide was 0.025%. Molecular characteristics of LVP were determined by gel permeation chromatography, FT-IR spectroscopy, and thin-layer chromatography. Our results revealed that LVP is a polysaccharide composed of only glucose units, and has a molecular weight of 336 kDa. Biological activity assays indicated that LVP exhibits both cytotoxic and antioxidant activity. LVP showed specific cytotoxicity against cancer cells (HeLa and HepG2 cells), and alterations in cancer cell morphology were found after LVP treatment.
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spelling pubmed-70481992020-03-10 Purification, Structural Characterization, and Biological Activity of Polysaccharides from Lentinus velutinus Udchumpisai, Wascharin Bangyeekhun, Eakaphun Mycobiology Research Article A polysaccharide (LVP) was purified from fruiting body of Lentinus velutinus by ethanol precipitation fractionation and DEAE and Sephadex G-100 column chromatography. The yield of purified polysaccharide was 0.025%. Molecular characteristics of LVP were determined by gel permeation chromatography, FT-IR spectroscopy, and thin-layer chromatography. Our results revealed that LVP is a polysaccharide composed of only glucose units, and has a molecular weight of 336 kDa. Biological activity assays indicated that LVP exhibits both cytotoxic and antioxidant activity. LVP showed specific cytotoxicity against cancer cells (HeLa and HepG2 cells), and alterations in cancer cell morphology were found after LVP treatment. Taylor & Francis 2019-11-29 /pmc/articles/PMC7048199/ /pubmed/32158606 http://dx.doi.org/10.1080/12298093.2019.1693482 Text en © 2019 The Author(s). Published by Informa UK Limited, trading as Taylor & Francis Group on behalf of the Korean Society of Mycology. http://creativecommons.org/licenses/by-nc/4.0/ This is an Open Access article distributed under the terms of the Creative Commons Attribution-NonCommercial License (http://creativecommons.org/licenses/by-nc/4.0/), which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Research Article
Udchumpisai, Wascharin
Bangyeekhun, Eakaphun
Purification, Structural Characterization, and Biological Activity of Polysaccharides from Lentinus velutinus
title Purification, Structural Characterization, and Biological Activity of Polysaccharides from Lentinus velutinus
title_full Purification, Structural Characterization, and Biological Activity of Polysaccharides from Lentinus velutinus
title_fullStr Purification, Structural Characterization, and Biological Activity of Polysaccharides from Lentinus velutinus
title_full_unstemmed Purification, Structural Characterization, and Biological Activity of Polysaccharides from Lentinus velutinus
title_short Purification, Structural Characterization, and Biological Activity of Polysaccharides from Lentinus velutinus
title_sort purification, structural characterization, and biological activity of polysaccharides from lentinus velutinus
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7048199/
https://www.ncbi.nlm.nih.gov/pubmed/32158606
http://dx.doi.org/10.1080/12298093.2019.1693482
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