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Small extracellular vesicles deliver miR-21 and miR-217 as pro-senescence effectors to endothelial cells

The role of epigenetics in endothelial cell senescence is a cutting-edge topic in ageing research. However, little is known of the relative contribution to pro-senescence signal propagation provided by microRNAs shuttled by extracellular vesicles (EVs) released from senescent cells. Analysis of micr...

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Autores principales: Mensà, Emanuela, Guescini, Michele, Giuliani, Angelica, Bacalini, Maria Giulia, Ramini, Deborah, Corleone, Giacomo, Ferracin, Manuela, Fulgenzi, Gianluca, Graciotti, Laura, Prattichizzo, Francesco, Sorci, Leonardo, Battistelli, Michela, Monsurrò, Vladia, Bonfigli, Anna Rita, Cardelli, Maurizio, Recchioni, Rina, Marcheselli, Fiorella, Latini, Silvia, Maggio, Serena, Fanelli, Mirco, Amatori, Stefano, Storci, Gianluca, Ceriello, Antonio, Stocchi, Vilberto, De Luca, Maria, Magnani, Luca, Rippo, Maria Rita, Procopio, Antonio Domenico, Sala, Claudia, Budimir, Iva, Bassi, Cristian, Negrini, Massimo, Garagnani, Paolo, Franceschi, Claudio, Sabbatinelli, Jacopo, Bonafè, Massimiliano, Olivieri, Fabiola
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Taylor & Francis 2020
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7048230/
https://www.ncbi.nlm.nih.gov/pubmed/32158519
http://dx.doi.org/10.1080/20013078.2020.1725285
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author Mensà, Emanuela
Guescini, Michele
Giuliani, Angelica
Bacalini, Maria Giulia
Ramini, Deborah
Corleone, Giacomo
Ferracin, Manuela
Fulgenzi, Gianluca
Graciotti, Laura
Prattichizzo, Francesco
Sorci, Leonardo
Battistelli, Michela
Monsurrò, Vladia
Bonfigli, Anna Rita
Cardelli, Maurizio
Recchioni, Rina
Marcheselli, Fiorella
Latini, Silvia
Maggio, Serena
Fanelli, Mirco
Amatori, Stefano
Storci, Gianluca
Ceriello, Antonio
Stocchi, Vilberto
De Luca, Maria
Magnani, Luca
Rippo, Maria Rita
Procopio, Antonio Domenico
Sala, Claudia
Budimir, Iva
Bassi, Cristian
Negrini, Massimo
Garagnani, Paolo
Franceschi, Claudio
Sabbatinelli, Jacopo
Bonafè, Massimiliano
Olivieri, Fabiola
author_facet Mensà, Emanuela
Guescini, Michele
Giuliani, Angelica
Bacalini, Maria Giulia
Ramini, Deborah
Corleone, Giacomo
Ferracin, Manuela
Fulgenzi, Gianluca
Graciotti, Laura
Prattichizzo, Francesco
Sorci, Leonardo
Battistelli, Michela
Monsurrò, Vladia
Bonfigli, Anna Rita
Cardelli, Maurizio
Recchioni, Rina
Marcheselli, Fiorella
Latini, Silvia
Maggio, Serena
Fanelli, Mirco
Amatori, Stefano
Storci, Gianluca
Ceriello, Antonio
Stocchi, Vilberto
De Luca, Maria
Magnani, Luca
Rippo, Maria Rita
Procopio, Antonio Domenico
Sala, Claudia
Budimir, Iva
Bassi, Cristian
Negrini, Massimo
Garagnani, Paolo
Franceschi, Claudio
Sabbatinelli, Jacopo
Bonafè, Massimiliano
Olivieri, Fabiola
author_sort Mensà, Emanuela
collection PubMed
description The role of epigenetics in endothelial cell senescence is a cutting-edge topic in ageing research. However, little is known of the relative contribution to pro-senescence signal propagation provided by microRNAs shuttled by extracellular vesicles (EVs) released from senescent cells. Analysis of microRNA and DNA methylation profiles in non-senescent (control) and senescent (SEN) human umbilical vein endothelial cells (HUVECs), and microRNA profiling of their cognate small EVs (sEVs) and large EVs demonstrated that SEN cells released a significantly greater sEV number than control cells. sEVs were enriched in miR-21-5p and miR-217, which target DNMT1 and SIRT1. Treatment of control cells with SEN sEVs induced a miR-21/miR-217-related impairment of DNMT1-SIRT1 expression, the reduction of proliferation markers, the acquisition of a senescent phenotype and a partial demethylation of the locus encoding for miR-21. MicroRNA profiling of sEVs from plasma of healthy subjects aged 40–100 years showed an inverse U-shaped age-related trend for miR-21-5p, consistent with senescence-associated biomarker profiles. Our findings suggest that miR-21-5p/miR-217 carried by SEN sEVs spread pro-senescence signals, affecting DNA methylation and cell replication.
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spelling pubmed-70482302020-03-10 Small extracellular vesicles deliver miR-21 and miR-217 as pro-senescence effectors to endothelial cells Mensà, Emanuela Guescini, Michele Giuliani, Angelica Bacalini, Maria Giulia Ramini, Deborah Corleone, Giacomo Ferracin, Manuela Fulgenzi, Gianluca Graciotti, Laura Prattichizzo, Francesco Sorci, Leonardo Battistelli, Michela Monsurrò, Vladia Bonfigli, Anna Rita Cardelli, Maurizio Recchioni, Rina Marcheselli, Fiorella Latini, Silvia Maggio, Serena Fanelli, Mirco Amatori, Stefano Storci, Gianluca Ceriello, Antonio Stocchi, Vilberto De Luca, Maria Magnani, Luca Rippo, Maria Rita Procopio, Antonio Domenico Sala, Claudia Budimir, Iva Bassi, Cristian Negrini, Massimo Garagnani, Paolo Franceschi, Claudio Sabbatinelli, Jacopo Bonafè, Massimiliano Olivieri, Fabiola J Extracell Vesicles Research Article The role of epigenetics in endothelial cell senescence is a cutting-edge topic in ageing research. However, little is known of the relative contribution to pro-senescence signal propagation provided by microRNAs shuttled by extracellular vesicles (EVs) released from senescent cells. Analysis of microRNA and DNA methylation profiles in non-senescent (control) and senescent (SEN) human umbilical vein endothelial cells (HUVECs), and microRNA profiling of their cognate small EVs (sEVs) and large EVs demonstrated that SEN cells released a significantly greater sEV number than control cells. sEVs were enriched in miR-21-5p and miR-217, which target DNMT1 and SIRT1. Treatment of control cells with SEN sEVs induced a miR-21/miR-217-related impairment of DNMT1-SIRT1 expression, the reduction of proliferation markers, the acquisition of a senescent phenotype and a partial demethylation of the locus encoding for miR-21. MicroRNA profiling of sEVs from plasma of healthy subjects aged 40–100 years showed an inverse U-shaped age-related trend for miR-21-5p, consistent with senescence-associated biomarker profiles. Our findings suggest that miR-21-5p/miR-217 carried by SEN sEVs spread pro-senescence signals, affecting DNA methylation and cell replication. Taylor & Francis 2020-02-18 /pmc/articles/PMC7048230/ /pubmed/32158519 http://dx.doi.org/10.1080/20013078.2020.1725285 Text en © 2020 The Author(s). Published by Informa UK Limited, trading as Taylor & Francis Group on behalf of The International Society for Extracellular Vesicles. http://creativecommons.org/licenses/by-nc/4.0/ http://creativecommons.org/licenses/by-nc/4.0/This is an Open Access article distributed under the terms of the Creative Commons Attribution-NonCommercial License (http://creativecommons.org/licenses/by-nc/4.0/), which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Research Article
Mensà, Emanuela
Guescini, Michele
Giuliani, Angelica
Bacalini, Maria Giulia
Ramini, Deborah
Corleone, Giacomo
Ferracin, Manuela
Fulgenzi, Gianluca
Graciotti, Laura
Prattichizzo, Francesco
Sorci, Leonardo
Battistelli, Michela
Monsurrò, Vladia
Bonfigli, Anna Rita
Cardelli, Maurizio
Recchioni, Rina
Marcheselli, Fiorella
Latini, Silvia
Maggio, Serena
Fanelli, Mirco
Amatori, Stefano
Storci, Gianluca
Ceriello, Antonio
Stocchi, Vilberto
De Luca, Maria
Magnani, Luca
Rippo, Maria Rita
Procopio, Antonio Domenico
Sala, Claudia
Budimir, Iva
Bassi, Cristian
Negrini, Massimo
Garagnani, Paolo
Franceschi, Claudio
Sabbatinelli, Jacopo
Bonafè, Massimiliano
Olivieri, Fabiola
Small extracellular vesicles deliver miR-21 and miR-217 as pro-senescence effectors to endothelial cells
title Small extracellular vesicles deliver miR-21 and miR-217 as pro-senescence effectors to endothelial cells
title_full Small extracellular vesicles deliver miR-21 and miR-217 as pro-senescence effectors to endothelial cells
title_fullStr Small extracellular vesicles deliver miR-21 and miR-217 as pro-senescence effectors to endothelial cells
title_full_unstemmed Small extracellular vesicles deliver miR-21 and miR-217 as pro-senescence effectors to endothelial cells
title_short Small extracellular vesicles deliver miR-21 and miR-217 as pro-senescence effectors to endothelial cells
title_sort small extracellular vesicles deliver mir-21 and mir-217 as pro-senescence effectors to endothelial cells
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7048230/
https://www.ncbi.nlm.nih.gov/pubmed/32158519
http://dx.doi.org/10.1080/20013078.2020.1725285
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