Stem rust resistance in wheat is suppressed by a subunit of the mediator complex

Stem rust is an important disease of wheat that can be controlled using resistance genes. The gene SuSr-D1 identified in cultivar ‘Canthatch’ suppresses stem rust resistance. SuSr-D1 mutants are resistant to several races of stem rust that are virulent on wild-type plants. Here we identify SuSr-D1 b...

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Autores principales: Hiebert, Colin W., Moscou, Matthew J., Hewitt, Tim, Steuernagel, Burkhard, Hernández-Pinzón, Inma, Green, Phon, Pujol, Vincent, Zhang, Peng, Rouse, Matthew N., Jin, Yue, McIntosh, Robert A., Upadhyaya, Narayana, Zhang, Jianping, Bhavani, Sridhar, Vrána, Jan, Karafiátová, Miroslava, Huang, Li, Fetch, Tom, Doležel, Jaroslav, Wulff, Brande B. H., Lagudah, Evans, Spielmeyer, Wolfgang
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Nature Publishing Group UK 2020
Materias:
Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7048732/
https://www.ncbi.nlm.nih.gov/pubmed/32111840
http://dx.doi.org/10.1038/s41467-020-14937-2
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author Hiebert, Colin W.
Moscou, Matthew J.
Hewitt, Tim
Steuernagel, Burkhard
Hernández-Pinzón, Inma
Green, Phon
Pujol, Vincent
Zhang, Peng
Rouse, Matthew N.
Jin, Yue
McIntosh, Robert A.
Upadhyaya, Narayana
Zhang, Jianping
Bhavani, Sridhar
Vrána, Jan
Karafiátová, Miroslava
Huang, Li
Fetch, Tom
Doležel, Jaroslav
Wulff, Brande B. H.
Lagudah, Evans
Spielmeyer, Wolfgang
author_facet Hiebert, Colin W.
Moscou, Matthew J.
Hewitt, Tim
Steuernagel, Burkhard
Hernández-Pinzón, Inma
Green, Phon
Pujol, Vincent
Zhang, Peng
Rouse, Matthew N.
Jin, Yue
McIntosh, Robert A.
Upadhyaya, Narayana
Zhang, Jianping
Bhavani, Sridhar
Vrána, Jan
Karafiátová, Miroslava
Huang, Li
Fetch, Tom
Doležel, Jaroslav
Wulff, Brande B. H.
Lagudah, Evans
Spielmeyer, Wolfgang
author_sort Hiebert, Colin W.
collection PubMed
description Stem rust is an important disease of wheat that can be controlled using resistance genes. The gene SuSr-D1 identified in cultivar ‘Canthatch’ suppresses stem rust resistance. SuSr-D1 mutants are resistant to several races of stem rust that are virulent on wild-type plants. Here we identify SuSr-D1 by sequencing flow-sorted chromosomes, mutagenesis, and map-based cloning. The gene encodes Med15, a subunit of the Mediator Complex, a conserved protein complex in eukaryotes that regulates expression of protein-coding genes. Nonsense mutations in Med15b.D result in expression of stem rust resistance. Time-course RNAseq analysis show a significant reduction or complete loss of differential gene expression at 24 h post inoculation in med15b.D mutants, suggesting that transcriptional reprogramming at this time point is not required for immunity to stem rust. Suppression is a common phenomenon and this study provides novel insight into suppression of rust resistance in wheat.
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spelling pubmed-70487322020-03-02 Stem rust resistance in wheat is suppressed by a subunit of the mediator complex Hiebert, Colin W. Moscou, Matthew J. Hewitt, Tim Steuernagel, Burkhard Hernández-Pinzón, Inma Green, Phon Pujol, Vincent Zhang, Peng Rouse, Matthew N. Jin, Yue McIntosh, Robert A. Upadhyaya, Narayana Zhang, Jianping Bhavani, Sridhar Vrána, Jan Karafiátová, Miroslava Huang, Li Fetch, Tom Doležel, Jaroslav Wulff, Brande B. H. Lagudah, Evans Spielmeyer, Wolfgang Nat Commun Article Stem rust is an important disease of wheat that can be controlled using resistance genes. The gene SuSr-D1 identified in cultivar ‘Canthatch’ suppresses stem rust resistance. SuSr-D1 mutants are resistant to several races of stem rust that are virulent on wild-type plants. Here we identify SuSr-D1 by sequencing flow-sorted chromosomes, mutagenesis, and map-based cloning. The gene encodes Med15, a subunit of the Mediator Complex, a conserved protein complex in eukaryotes that regulates expression of protein-coding genes. Nonsense mutations in Med15b.D result in expression of stem rust resistance. Time-course RNAseq analysis show a significant reduction or complete loss of differential gene expression at 24 h post inoculation in med15b.D mutants, suggesting that transcriptional reprogramming at this time point is not required for immunity to stem rust. Suppression is a common phenomenon and this study provides novel insight into suppression of rust resistance in wheat. Nature Publishing Group UK 2020-02-28 /pmc/articles/PMC7048732/ /pubmed/32111840 http://dx.doi.org/10.1038/s41467-020-14937-2 Text en © Crown 2020 Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.
spellingShingle Article
Hiebert, Colin W.
Moscou, Matthew J.
Hewitt, Tim
Steuernagel, Burkhard
Hernández-Pinzón, Inma
Green, Phon
Pujol, Vincent
Zhang, Peng
Rouse, Matthew N.
Jin, Yue
McIntosh, Robert A.
Upadhyaya, Narayana
Zhang, Jianping
Bhavani, Sridhar
Vrána, Jan
Karafiátová, Miroslava
Huang, Li
Fetch, Tom
Doležel, Jaroslav
Wulff, Brande B. H.
Lagudah, Evans
Spielmeyer, Wolfgang
Stem rust resistance in wheat is suppressed by a subunit of the mediator complex
title Stem rust resistance in wheat is suppressed by a subunit of the mediator complex
title_full Stem rust resistance in wheat is suppressed by a subunit of the mediator complex
title_fullStr Stem rust resistance in wheat is suppressed by a subunit of the mediator complex
title_full_unstemmed Stem rust resistance in wheat is suppressed by a subunit of the mediator complex
title_short Stem rust resistance in wheat is suppressed by a subunit of the mediator complex
title_sort stem rust resistance in wheat is suppressed by a subunit of the mediator complex
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7048732/
https://www.ncbi.nlm.nih.gov/pubmed/32111840
http://dx.doi.org/10.1038/s41467-020-14937-2
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