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Quantum scale organic semiconductors for SERS detection of DNA methylation and gene expression
Cancer stem cells (CSC) can be identified by modifications in their genomic DNA. Here, we report a concept of precisely shrinking an organic semiconductor surface-enhanced Raman scattering (SERS) probe to quantum size, for investigating the epigenetic profile of CSC. The probe is used for tag-free g...
Autores principales: | , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Nature Publishing Group UK
2020
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7048788/ https://www.ncbi.nlm.nih.gov/pubmed/32111825 http://dx.doi.org/10.1038/s41467-020-14774-3 |
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author | Ganesh, Swarna Venkatakrishnan, Krishnan Tan, Bo |
author_facet | Ganesh, Swarna Venkatakrishnan, Krishnan Tan, Bo |
author_sort | Ganesh, Swarna |
collection | PubMed |
description | Cancer stem cells (CSC) can be identified by modifications in their genomic DNA. Here, we report a concept of precisely shrinking an organic semiconductor surface-enhanced Raman scattering (SERS) probe to quantum size, for investigating the epigenetic profile of CSC. The probe is used for tag-free genomic DNA detection, an approach towards the advancement of single-molecule DNA detection. The sensor detected structural, molecular and gene expression aberrations of genomic DNA in femtomolar concentration simultaneously in a single test. In addition to pointing out the divergences in genomic DNA of cancerous and non-cancerous cells, the quantum scale organic semiconductor was able to trace the expression of two genes which are frequently used as CSC markers. The quantum scale organic semiconductor holds the potential to be a new tool for label-free, ultra-sensitive multiplexed genomic analysis. |
format | Online Article Text |
id | pubmed-7048788 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2020 |
publisher | Nature Publishing Group UK |
record_format | MEDLINE/PubMed |
spelling | pubmed-70487882020-03-02 Quantum scale organic semiconductors for SERS detection of DNA methylation and gene expression Ganesh, Swarna Venkatakrishnan, Krishnan Tan, Bo Nat Commun Article Cancer stem cells (CSC) can be identified by modifications in their genomic DNA. Here, we report a concept of precisely shrinking an organic semiconductor surface-enhanced Raman scattering (SERS) probe to quantum size, for investigating the epigenetic profile of CSC. The probe is used for tag-free genomic DNA detection, an approach towards the advancement of single-molecule DNA detection. The sensor detected structural, molecular and gene expression aberrations of genomic DNA in femtomolar concentration simultaneously in a single test. In addition to pointing out the divergences in genomic DNA of cancerous and non-cancerous cells, the quantum scale organic semiconductor was able to trace the expression of two genes which are frequently used as CSC markers. The quantum scale organic semiconductor holds the potential to be a new tool for label-free, ultra-sensitive multiplexed genomic analysis. Nature Publishing Group UK 2020-02-28 /pmc/articles/PMC7048788/ /pubmed/32111825 http://dx.doi.org/10.1038/s41467-020-14774-3 Text en © The Author(s) 2020 Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/. |
spellingShingle | Article Ganesh, Swarna Venkatakrishnan, Krishnan Tan, Bo Quantum scale organic semiconductors for SERS detection of DNA methylation and gene expression |
title | Quantum scale organic semiconductors for SERS detection of DNA methylation and gene expression |
title_full | Quantum scale organic semiconductors for SERS detection of DNA methylation and gene expression |
title_fullStr | Quantum scale organic semiconductors for SERS detection of DNA methylation and gene expression |
title_full_unstemmed | Quantum scale organic semiconductors for SERS detection of DNA methylation and gene expression |
title_short | Quantum scale organic semiconductors for SERS detection of DNA methylation and gene expression |
title_sort | quantum scale organic semiconductors for sers detection of dna methylation and gene expression |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7048788/ https://www.ncbi.nlm.nih.gov/pubmed/32111825 http://dx.doi.org/10.1038/s41467-020-14774-3 |
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