Cargando…
Inhalation of lung spheroid cell secretome and exosomes promotes lung repair in pulmonary fibrosis
Idiopathic pulmonary fibrosis (IPF) is a fatal and incurable form of interstitial lung disease in which persistent injury results in scar tissue formation. As fibrosis thickens, the lung tissue loses the ability to facilitate gas exchange and provide cells with needed oxygen. Currently, IPF has few...
Autores principales: | , , , , , , , , , , , , , , , , , , , , , |
---|---|
Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Nature Publishing Group UK
2020
|
Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7048814/ https://www.ncbi.nlm.nih.gov/pubmed/32111836 http://dx.doi.org/10.1038/s41467-020-14344-7 |
_version_ | 1783502340623433728 |
---|---|
author | Dinh, Phuong-Uyen C. Paudel, Dipti Brochu, Hayden Popowski, Kristen D. Gracieux, M. Cyndell Cores, Jhon Huang, Ke Hensley, M. Taylor Harrell, Erin Vandergriff, Adam C. George, Arianna K. Barrio, Raina T. Hu, Shiqi Allen, Tyler A. Blackburn, Kevin Caranasos, Thomas G. Peng, Xinxia Schnabel, Lauren V. Adler, Kenneth B. Lobo, Leonard J. Goshe, Michael B. Cheng, Ke |
author_facet | Dinh, Phuong-Uyen C. Paudel, Dipti Brochu, Hayden Popowski, Kristen D. Gracieux, M. Cyndell Cores, Jhon Huang, Ke Hensley, M. Taylor Harrell, Erin Vandergriff, Adam C. George, Arianna K. Barrio, Raina T. Hu, Shiqi Allen, Tyler A. Blackburn, Kevin Caranasos, Thomas G. Peng, Xinxia Schnabel, Lauren V. Adler, Kenneth B. Lobo, Leonard J. Goshe, Michael B. Cheng, Ke |
author_sort | Dinh, Phuong-Uyen C. |
collection | PubMed |
description | Idiopathic pulmonary fibrosis (IPF) is a fatal and incurable form of interstitial lung disease in which persistent injury results in scar tissue formation. As fibrosis thickens, the lung tissue loses the ability to facilitate gas exchange and provide cells with needed oxygen. Currently, IPF has few treatment options and no effective therapies, aside from lung transplant. Here we present a series of studies utilizing lung spheroid cell-secretome (LSC-Sec) and exosomes (LSC-Exo) by inhalation to treat different models of lung injury and fibrosis. Analysis reveals that LSC-Sec and LSC-Exo treatments could attenuate and resolve bleomycin- and silica-induced fibrosis by reestablishing normal alveolar structure and decreasing both collagen accumulation and myofibroblast proliferation. Additionally, LSC-Sec and LSC-Exo exhibit superior therapeutic benefits than their counterparts derived from mesenchymal stem cells in some measures. We showed that an inhalation treatment of secretome and exosome exhibited therapeutic potential for lung regeneration in two experimental models of pulmonary fibrosis. |
format | Online Article Text |
id | pubmed-7048814 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2020 |
publisher | Nature Publishing Group UK |
record_format | MEDLINE/PubMed |
spelling | pubmed-70488142020-03-02 Inhalation of lung spheroid cell secretome and exosomes promotes lung repair in pulmonary fibrosis Dinh, Phuong-Uyen C. Paudel, Dipti Brochu, Hayden Popowski, Kristen D. Gracieux, M. Cyndell Cores, Jhon Huang, Ke Hensley, M. Taylor Harrell, Erin Vandergriff, Adam C. George, Arianna K. Barrio, Raina T. Hu, Shiqi Allen, Tyler A. Blackburn, Kevin Caranasos, Thomas G. Peng, Xinxia Schnabel, Lauren V. Adler, Kenneth B. Lobo, Leonard J. Goshe, Michael B. Cheng, Ke Nat Commun Article Idiopathic pulmonary fibrosis (IPF) is a fatal and incurable form of interstitial lung disease in which persistent injury results in scar tissue formation. As fibrosis thickens, the lung tissue loses the ability to facilitate gas exchange and provide cells with needed oxygen. Currently, IPF has few treatment options and no effective therapies, aside from lung transplant. Here we present a series of studies utilizing lung spheroid cell-secretome (LSC-Sec) and exosomes (LSC-Exo) by inhalation to treat different models of lung injury and fibrosis. Analysis reveals that LSC-Sec and LSC-Exo treatments could attenuate and resolve bleomycin- and silica-induced fibrosis by reestablishing normal alveolar structure and decreasing both collagen accumulation and myofibroblast proliferation. Additionally, LSC-Sec and LSC-Exo exhibit superior therapeutic benefits than their counterparts derived from mesenchymal stem cells in some measures. We showed that an inhalation treatment of secretome and exosome exhibited therapeutic potential for lung regeneration in two experimental models of pulmonary fibrosis. Nature Publishing Group UK 2020-02-28 /pmc/articles/PMC7048814/ /pubmed/32111836 http://dx.doi.org/10.1038/s41467-020-14344-7 Text en © The Author(s) 2020 Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/. |
spellingShingle | Article Dinh, Phuong-Uyen C. Paudel, Dipti Brochu, Hayden Popowski, Kristen D. Gracieux, M. Cyndell Cores, Jhon Huang, Ke Hensley, M. Taylor Harrell, Erin Vandergriff, Adam C. George, Arianna K. Barrio, Raina T. Hu, Shiqi Allen, Tyler A. Blackburn, Kevin Caranasos, Thomas G. Peng, Xinxia Schnabel, Lauren V. Adler, Kenneth B. Lobo, Leonard J. Goshe, Michael B. Cheng, Ke Inhalation of lung spheroid cell secretome and exosomes promotes lung repair in pulmonary fibrosis |
title | Inhalation of lung spheroid cell secretome and exosomes promotes lung repair in pulmonary fibrosis |
title_full | Inhalation of lung spheroid cell secretome and exosomes promotes lung repair in pulmonary fibrosis |
title_fullStr | Inhalation of lung spheroid cell secretome and exosomes promotes lung repair in pulmonary fibrosis |
title_full_unstemmed | Inhalation of lung spheroid cell secretome and exosomes promotes lung repair in pulmonary fibrosis |
title_short | Inhalation of lung spheroid cell secretome and exosomes promotes lung repair in pulmonary fibrosis |
title_sort | inhalation of lung spheroid cell secretome and exosomes promotes lung repair in pulmonary fibrosis |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7048814/ https://www.ncbi.nlm.nih.gov/pubmed/32111836 http://dx.doi.org/10.1038/s41467-020-14344-7 |
work_keys_str_mv | AT dinhphuonguyenc inhalationoflungspheroidcellsecretomeandexosomespromoteslungrepairinpulmonaryfibrosis AT paudeldipti inhalationoflungspheroidcellsecretomeandexosomespromoteslungrepairinpulmonaryfibrosis AT brochuhayden inhalationoflungspheroidcellsecretomeandexosomespromoteslungrepairinpulmonaryfibrosis AT popowskikristend inhalationoflungspheroidcellsecretomeandexosomespromoteslungrepairinpulmonaryfibrosis AT gracieuxmcyndell inhalationoflungspheroidcellsecretomeandexosomespromoteslungrepairinpulmonaryfibrosis AT coresjhon inhalationoflungspheroidcellsecretomeandexosomespromoteslungrepairinpulmonaryfibrosis AT huangke inhalationoflungspheroidcellsecretomeandexosomespromoteslungrepairinpulmonaryfibrosis AT hensleymtaylor inhalationoflungspheroidcellsecretomeandexosomespromoteslungrepairinpulmonaryfibrosis AT harrellerin inhalationoflungspheroidcellsecretomeandexosomespromoteslungrepairinpulmonaryfibrosis AT vandergriffadamc inhalationoflungspheroidcellsecretomeandexosomespromoteslungrepairinpulmonaryfibrosis AT georgeariannak inhalationoflungspheroidcellsecretomeandexosomespromoteslungrepairinpulmonaryfibrosis AT barriorainat inhalationoflungspheroidcellsecretomeandexosomespromoteslungrepairinpulmonaryfibrosis AT hushiqi inhalationoflungspheroidcellsecretomeandexosomespromoteslungrepairinpulmonaryfibrosis AT allentylera inhalationoflungspheroidcellsecretomeandexosomespromoteslungrepairinpulmonaryfibrosis AT blackburnkevin inhalationoflungspheroidcellsecretomeandexosomespromoteslungrepairinpulmonaryfibrosis AT caranasosthomasg inhalationoflungspheroidcellsecretomeandexosomespromoteslungrepairinpulmonaryfibrosis AT pengxinxia inhalationoflungspheroidcellsecretomeandexosomespromoteslungrepairinpulmonaryfibrosis AT schnabellaurenv inhalationoflungspheroidcellsecretomeandexosomespromoteslungrepairinpulmonaryfibrosis AT adlerkennethb inhalationoflungspheroidcellsecretomeandexosomespromoteslungrepairinpulmonaryfibrosis AT loboleonardj inhalationoflungspheroidcellsecretomeandexosomespromoteslungrepairinpulmonaryfibrosis AT goshemichaelb inhalationoflungspheroidcellsecretomeandexosomespromoteslungrepairinpulmonaryfibrosis AT chengke inhalationoflungspheroidcellsecretomeandexosomespromoteslungrepairinpulmonaryfibrosis |