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Distribution and genetic diversity of adeno-associated viruses in bats from coastal areas of Southeast China
Bats are associated with several important zoonotic viruses from different families. One example includes adeno-associated viruses (AAVs), that are extensively detected in several animals, especially primates. To understand AAVs distribution and genetic diversity in the coastal areas of Southeast Ch...
Autores principales: | , , , , , , , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Nature Publishing Group UK
2020
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7048818/ https://www.ncbi.nlm.nih.gov/pubmed/32111911 http://dx.doi.org/10.1038/s41598-020-60721-z |
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author | Zhu, Changqiang Wang, Chunhui Wu, Jiahong Ye, Fuqiang Lv, Ruichen Hu, Dan Ai, Lele Yang, Lu Wu, Ting Li, Bo Ding, Chenxi Zhang, Bin Lv, Heng Wang, Changjun Tan, Weilong |
author_facet | Zhu, Changqiang Wang, Chunhui Wu, Jiahong Ye, Fuqiang Lv, Ruichen Hu, Dan Ai, Lele Yang, Lu Wu, Ting Li, Bo Ding, Chenxi Zhang, Bin Lv, Heng Wang, Changjun Tan, Weilong |
author_sort | Zhu, Changqiang |
collection | PubMed |
description | Bats are associated with several important zoonotic viruses from different families. One example includes adeno-associated viruses (AAVs), that are extensively detected in several animals, especially primates. To understand AAVs distribution and genetic diversity in the coastal areas of Southeast China, a total of 415 intestine samples were mostly collected from two provinces of southeast China, i.e., Zhejiang and Fujian province. Intestine samples from five bat species were collected for AAVs detection. The average prevalence rate for AAV detection among these samples was 18.6% (77 positives out of 415 samples) and ranged from 11.8 to 28.9% between the five bat species. This suggests that AAVs are widely distributed in diverse bat populations in southeast coastal areas of China. Based on the genome sequence of bat adeno-associated virus-CXC1(BtAAV-CXC1) from one AAV-positive sample, the genetic diversity of the detected AAVs were assessed and analyzed. Phylogenetic analysis revealed that BtAAV-CXC1 was comparatively distant to other major AAVs from mammals and non-mammals, with only a 52.9~64.7% nucleotide identity. However, they were phylogenetically closer to Rhinolophus sinicus bat adeno-associated virus (Rs-BtAAV1), with a 74.5% nt similarity. Partial analysis of the rep and cap overlapping open reading frame (ORF) sequences from bat AAV samples revealed 48 partial rep sequences and 23 partial cap sequences from positive samples shared 86.9 to 100% and 72.3 to 98.8% nucleotide identities among themselves, respectively. This suggests that the detected AAVs had a distinctly high genetic diversity. These findings led us to conclude that diverse AAVs may be widely distributed in bat populations from the southeast regions of China. |
format | Online Article Text |
id | pubmed-7048818 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2020 |
publisher | Nature Publishing Group UK |
record_format | MEDLINE/PubMed |
spelling | pubmed-70488182020-03-06 Distribution and genetic diversity of adeno-associated viruses in bats from coastal areas of Southeast China Zhu, Changqiang Wang, Chunhui Wu, Jiahong Ye, Fuqiang Lv, Ruichen Hu, Dan Ai, Lele Yang, Lu Wu, Ting Li, Bo Ding, Chenxi Zhang, Bin Lv, Heng Wang, Changjun Tan, Weilong Sci Rep Article Bats are associated with several important zoonotic viruses from different families. One example includes adeno-associated viruses (AAVs), that are extensively detected in several animals, especially primates. To understand AAVs distribution and genetic diversity in the coastal areas of Southeast China, a total of 415 intestine samples were mostly collected from two provinces of southeast China, i.e., Zhejiang and Fujian province. Intestine samples from five bat species were collected for AAVs detection. The average prevalence rate for AAV detection among these samples was 18.6% (77 positives out of 415 samples) and ranged from 11.8 to 28.9% between the five bat species. This suggests that AAVs are widely distributed in diverse bat populations in southeast coastal areas of China. Based on the genome sequence of bat adeno-associated virus-CXC1(BtAAV-CXC1) from one AAV-positive sample, the genetic diversity of the detected AAVs were assessed and analyzed. Phylogenetic analysis revealed that BtAAV-CXC1 was comparatively distant to other major AAVs from mammals and non-mammals, with only a 52.9~64.7% nucleotide identity. However, they were phylogenetically closer to Rhinolophus sinicus bat adeno-associated virus (Rs-BtAAV1), with a 74.5% nt similarity. Partial analysis of the rep and cap overlapping open reading frame (ORF) sequences from bat AAV samples revealed 48 partial rep sequences and 23 partial cap sequences from positive samples shared 86.9 to 100% and 72.3 to 98.8% nucleotide identities among themselves, respectively. This suggests that the detected AAVs had a distinctly high genetic diversity. These findings led us to conclude that diverse AAVs may be widely distributed in bat populations from the southeast regions of China. Nature Publishing Group UK 2020-02-28 /pmc/articles/PMC7048818/ /pubmed/32111911 http://dx.doi.org/10.1038/s41598-020-60721-z Text en © The Author(s) 2020 Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/. |
spellingShingle | Article Zhu, Changqiang Wang, Chunhui Wu, Jiahong Ye, Fuqiang Lv, Ruichen Hu, Dan Ai, Lele Yang, Lu Wu, Ting Li, Bo Ding, Chenxi Zhang, Bin Lv, Heng Wang, Changjun Tan, Weilong Distribution and genetic diversity of adeno-associated viruses in bats from coastal areas of Southeast China |
title | Distribution and genetic diversity of adeno-associated viruses in bats from coastal areas of Southeast China |
title_full | Distribution and genetic diversity of adeno-associated viruses in bats from coastal areas of Southeast China |
title_fullStr | Distribution and genetic diversity of adeno-associated viruses in bats from coastal areas of Southeast China |
title_full_unstemmed | Distribution and genetic diversity of adeno-associated viruses in bats from coastal areas of Southeast China |
title_short | Distribution and genetic diversity of adeno-associated viruses in bats from coastal areas of Southeast China |
title_sort | distribution and genetic diversity of adeno-associated viruses in bats from coastal areas of southeast china |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7048818/ https://www.ncbi.nlm.nih.gov/pubmed/32111911 http://dx.doi.org/10.1038/s41598-020-60721-z |
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