Cargando…

Probiotics modulate the microbiota–gut–brain axis and improve memory deficits in aged SAMP8 mice

ProBiotic-4 is a probiotic preparation composed of Bifidobacterium lactis, Lactobacillus casei, Bifidobacterium bifidum, and Lactobacillus acidophilus. This study aims to investigate the effects of ProBiotic-4 on the microbiota–gut–brain axis and cognitive deficits, and to explore the underlying mol...

Descripción completa

Detalles Bibliográficos
Autores principales: Yang, Xueqin, Yu, Dongke, Xue, Li, Li, Hui, Du, Junrong
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Elsevier 2020
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7049608/
https://www.ncbi.nlm.nih.gov/pubmed/32140393
http://dx.doi.org/10.1016/j.apsb.2019.07.001
_version_ 1783502472807972864
author Yang, Xueqin
Yu, Dongke
Xue, Li
Li, Hui
Du, Junrong
author_facet Yang, Xueqin
Yu, Dongke
Xue, Li
Li, Hui
Du, Junrong
author_sort Yang, Xueqin
collection PubMed
description ProBiotic-4 is a probiotic preparation composed of Bifidobacterium lactis, Lactobacillus casei, Bifidobacterium bifidum, and Lactobacillus acidophilus. This study aims to investigate the effects of ProBiotic-4 on the microbiota–gut–brain axis and cognitive deficits, and to explore the underlying molecular mechanism using senescence-accelerated mouse prone 8 (SAMP8) mice. ProBiotic-4 was orally administered to 9-month-old SAMP8 mice for 12 weeks. We observed that ProBiotic-4 significantly improved the memory deficits, cerebral neuronal and synaptic injuries, glial activation, and microbiota composition in the feces and brains of aged SAMP8 mice. ProBiotic-4 substantially attenuated aging-related disruption of the intestinal barrier and blood–brain barrier, decreased interleukin-6 and tumor necrosis factor-α at both mRNA and protein levels, reduced plasma and cerebral lipopolysaccharide (LPS) concentration, toll-like receptor 4 (TLR4) expression, and nuclear factor-κB (NF-κB) nuclear translocation in the brain. In addition, not only did ProBiotic-4 significantly decreased the levels of γ-H2AX, 8-hydroxydesoxyguanosine, and retinoic-acid-inducible gene-I (RIG-I), it also abrogated RIG-I multimerization in the brain. These findings suggest that targeting gut microbiota with probiotics may have a therapeutic potential for the deficits of the microbiota–gut–brain axis and cognitive function in aging, and that its mechanism is associated with inhibition of both TLR4-and RIG-I-mediated NF-κB signaling pathway and inflammatory responses.
format Online
Article
Text
id pubmed-7049608
institution National Center for Biotechnology Information
language English
publishDate 2020
publisher Elsevier
record_format MEDLINE/PubMed
spelling pubmed-70496082020-03-05 Probiotics modulate the microbiota–gut–brain axis and improve memory deficits in aged SAMP8 mice Yang, Xueqin Yu, Dongke Xue, Li Li, Hui Du, Junrong Acta Pharm Sin B Original article ProBiotic-4 is a probiotic preparation composed of Bifidobacterium lactis, Lactobacillus casei, Bifidobacterium bifidum, and Lactobacillus acidophilus. This study aims to investigate the effects of ProBiotic-4 on the microbiota–gut–brain axis and cognitive deficits, and to explore the underlying molecular mechanism using senescence-accelerated mouse prone 8 (SAMP8) mice. ProBiotic-4 was orally administered to 9-month-old SAMP8 mice for 12 weeks. We observed that ProBiotic-4 significantly improved the memory deficits, cerebral neuronal and synaptic injuries, glial activation, and microbiota composition in the feces and brains of aged SAMP8 mice. ProBiotic-4 substantially attenuated aging-related disruption of the intestinal barrier and blood–brain barrier, decreased interleukin-6 and tumor necrosis factor-α at both mRNA and protein levels, reduced plasma and cerebral lipopolysaccharide (LPS) concentration, toll-like receptor 4 (TLR4) expression, and nuclear factor-κB (NF-κB) nuclear translocation in the brain. In addition, not only did ProBiotic-4 significantly decreased the levels of γ-H2AX, 8-hydroxydesoxyguanosine, and retinoic-acid-inducible gene-I (RIG-I), it also abrogated RIG-I multimerization in the brain. These findings suggest that targeting gut microbiota with probiotics may have a therapeutic potential for the deficits of the microbiota–gut–brain axis and cognitive function in aging, and that its mechanism is associated with inhibition of both TLR4-and RIG-I-mediated NF-κB signaling pathway and inflammatory responses. Elsevier 2020-03 2019-07-07 /pmc/articles/PMC7049608/ /pubmed/32140393 http://dx.doi.org/10.1016/j.apsb.2019.07.001 Text en © 2020 Chinese Pharmaceutical Association and Institute of Materia Medica, Chinese Academy of Medical Sciences. Production and hosting by Elsevier B.V. http://creativecommons.org/licenses/by-nc-nd/4.0/ This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/).
spellingShingle Original article
Yang, Xueqin
Yu, Dongke
Xue, Li
Li, Hui
Du, Junrong
Probiotics modulate the microbiota–gut–brain axis and improve memory deficits in aged SAMP8 mice
title Probiotics modulate the microbiota–gut–brain axis and improve memory deficits in aged SAMP8 mice
title_full Probiotics modulate the microbiota–gut–brain axis and improve memory deficits in aged SAMP8 mice
title_fullStr Probiotics modulate the microbiota–gut–brain axis and improve memory deficits in aged SAMP8 mice
title_full_unstemmed Probiotics modulate the microbiota–gut–brain axis and improve memory deficits in aged SAMP8 mice
title_short Probiotics modulate the microbiota–gut–brain axis and improve memory deficits in aged SAMP8 mice
title_sort probiotics modulate the microbiota–gut–brain axis and improve memory deficits in aged samp8 mice
topic Original article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7049608/
https://www.ncbi.nlm.nih.gov/pubmed/32140393
http://dx.doi.org/10.1016/j.apsb.2019.07.001
work_keys_str_mv AT yangxueqin probioticsmodulatethemicrobiotagutbrainaxisandimprovememorydeficitsinagedsamp8mice
AT yudongke probioticsmodulatethemicrobiotagutbrainaxisandimprovememorydeficitsinagedsamp8mice
AT xueli probioticsmodulatethemicrobiotagutbrainaxisandimprovememorydeficitsinagedsamp8mice
AT lihui probioticsmodulatethemicrobiotagutbrainaxisandimprovememorydeficitsinagedsamp8mice
AT dujunrong probioticsmodulatethemicrobiotagutbrainaxisandimprovememorydeficitsinagedsamp8mice