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CRISPR-Cas12a based internal negative control for nonspecific products of exponential rolling circle amplification
False-positive results cause a major problem in nucleic acid amplification, and require external blank/negative controls for every test. However, external controls usually have a simpler and lower background compared to the test sample, resulting in underestimation of false-positive risks. Internal...
Autores principales: | , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Oxford University Press
2020
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7049689/ https://www.ncbi.nlm.nih.gov/pubmed/31956898 http://dx.doi.org/10.1093/nar/gkaa017 |