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CRISPR-Cas12a based internal negative control for nonspecific products of exponential rolling circle amplification

False-positive results cause a major problem in nucleic acid amplification, and require external blank/negative controls for every test. However, external controls usually have a simpler and lower background compared to the test sample, resulting in underestimation of false-positive risks. Internal...

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Detalles Bibliográficos
Autores principales: Tian, Bo, Minero, Gabriel Antonio S, Fock, Jeppe, Dufva, Martin, Hansen, Mikkel Fougt
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Oxford University Press 2020
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7049689/
https://www.ncbi.nlm.nih.gov/pubmed/31956898
http://dx.doi.org/10.1093/nar/gkaa017

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