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I-Block: a simple Escherichia coli-based assay for studying sequence-specific DNA binding of proteins

We have developed a simple method called I-Block assay, which can detect sequence-specific binding of proteins to DNA in Escherichia coli. The method works by detecting competition between the protein of interest and RNA polymerase for binding to overlapping target sites in a plasmid-borne lacI prom...

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Detalles Bibliográficos
Autores principales:	Szentes, Sarolta, Zsibrita, Nikolett, Koncz, Mihály, Zsigmond, Eszter, Salamon, Pál, Pletl, Zita, Kiss, Antal
Formato:	Online Artículo Texto
Lenguaje:	English
Publicado:	Oxford University Press 2020
Materias:	Methods Online
Acceso en línea:	https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7049694/ https://www.ncbi.nlm.nih.gov/pubmed/31980824 http://dx.doi.org/10.1093/nar/gkaa014

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