Cargando…
Clinical Relevance and Antimicrobial Profiling of Methicillin-Resistant Staphylococcus aureus (MRSA) on Routine Antibiotics and Ethanol Extract of Mango Kernel (Mangifera indica L.)
Methicillin-resistant Staphylococcus aureus (MRSA) is known for serious health problems. Testing new inexpensive natural products such as mango kernel (Mangifera indica L., Anacardiaceae) may provide alternative and economically viable anti-MRSA drugs. In the current study, we screened clinical isol...
Autores principales: | , , , , , |
---|---|
Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Hindawi
2020
|
Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7049837/ https://www.ncbi.nlm.nih.gov/pubmed/32149104 http://dx.doi.org/10.1155/2020/4150678 |
_version_ | 1783502525930930176 |
---|---|
author | Al Bshabshe, Ali Joseph, Martin R. P. Awad El-Gied, Amgad A. Fadul, Abdalla N. Chandramoorthy, Harish C. Hamid, Mohamed E. |
author_facet | Al Bshabshe, Ali Joseph, Martin R. P. Awad El-Gied, Amgad A. Fadul, Abdalla N. Chandramoorthy, Harish C. Hamid, Mohamed E. |
author_sort | Al Bshabshe, Ali |
collection | PubMed |
description | Methicillin-resistant Staphylococcus aureus (MRSA) is known for serious health problems. Testing new inexpensive natural products such as mango kernel (Mangifera indica L., Anacardiaceae) may provide alternative and economically viable anti-MRSA drugs. In the current study, we screened clinical isolates from Aseer Central Hospital, Saudi Arabia, during 2012–2017 for MRSA and tested an ethanolic extract of mango kernel for anti-MRSA activity. Brief confirmation of MRSA was performed by the Vitek 2 system, while antibiotic sensitivity of strains was tested for their clinical relevance. The In vitro disc diffusion method was used to test the anti-MRSA activity of the ethanolic mango kernel extract. The antimicrobial activity of mango kernel was compared to that of standard drugs (oxacillin and vancomycin). Of the identified 132 S. aureus strains, 42 (31.8%) were found to be MRSA and their prevalence showed a clear increase during the last two years (2016-2017; p < 0.001). MRSA strains showed 100% sensitivity to vancomycin, teicoplanin, linezolid, tetracycline, daptomycin, tigecycline, and tobramycin and 100% resistance to ampicillin and 98% to penicillin. The ethanolic extracts of mango kernel were found active against both S. aureus and the MRSA strains. Inhibitory activities (mean ± SE) were achieved at concentrations of 50 mg/mL (20.77 ± 0.61), 5 mg/mL (16.18 ± 0.34), and 0.5 mg/mL (8.39 ± 0.33) exceeding that of vancomycin (p=0.0162). MRSA strains were sensitive to mango kernel extracts when compared to vancomycin. Therefore, ethanolic extracts of mango kernel can be escalated to animal model studies as a promising leading anti-MRSA drug candidate and can be an economic alternative to high-priced synthetic antibiotics. |
format | Online Article Text |
id | pubmed-7049837 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2020 |
publisher | Hindawi |
record_format | MEDLINE/PubMed |
spelling | pubmed-70498372020-03-08 Clinical Relevance and Antimicrobial Profiling of Methicillin-Resistant Staphylococcus aureus (MRSA) on Routine Antibiotics and Ethanol Extract of Mango Kernel (Mangifera indica L.) Al Bshabshe, Ali Joseph, Martin R. P. Awad El-Gied, Amgad A. Fadul, Abdalla N. Chandramoorthy, Harish C. Hamid, Mohamed E. Biomed Res Int Research Article Methicillin-resistant Staphylococcus aureus (MRSA) is known for serious health problems. Testing new inexpensive natural products such as mango kernel (Mangifera indica L., Anacardiaceae) may provide alternative and economically viable anti-MRSA drugs. In the current study, we screened clinical isolates from Aseer Central Hospital, Saudi Arabia, during 2012–2017 for MRSA and tested an ethanolic extract of mango kernel for anti-MRSA activity. Brief confirmation of MRSA was performed by the Vitek 2 system, while antibiotic sensitivity of strains was tested for their clinical relevance. The In vitro disc diffusion method was used to test the anti-MRSA activity of the ethanolic mango kernel extract. The antimicrobial activity of mango kernel was compared to that of standard drugs (oxacillin and vancomycin). Of the identified 132 S. aureus strains, 42 (31.8%) were found to be MRSA and their prevalence showed a clear increase during the last two years (2016-2017; p < 0.001). MRSA strains showed 100% sensitivity to vancomycin, teicoplanin, linezolid, tetracycline, daptomycin, tigecycline, and tobramycin and 100% resistance to ampicillin and 98% to penicillin. The ethanolic extracts of mango kernel were found active against both S. aureus and the MRSA strains. Inhibitory activities (mean ± SE) were achieved at concentrations of 50 mg/mL (20.77 ± 0.61), 5 mg/mL (16.18 ± 0.34), and 0.5 mg/mL (8.39 ± 0.33) exceeding that of vancomycin (p=0.0162). MRSA strains were sensitive to mango kernel extracts when compared to vancomycin. Therefore, ethanolic extracts of mango kernel can be escalated to animal model studies as a promising leading anti-MRSA drug candidate and can be an economic alternative to high-priced synthetic antibiotics. Hindawi 2020-02-18 /pmc/articles/PMC7049837/ /pubmed/32149104 http://dx.doi.org/10.1155/2020/4150678 Text en Copyright © 2020 Ali Al Bshabshe et al. http://creativecommons.org/licenses/by/4.0/ This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. |
spellingShingle | Research Article Al Bshabshe, Ali Joseph, Martin R. P. Awad El-Gied, Amgad A. Fadul, Abdalla N. Chandramoorthy, Harish C. Hamid, Mohamed E. Clinical Relevance and Antimicrobial Profiling of Methicillin-Resistant Staphylococcus aureus (MRSA) on Routine Antibiotics and Ethanol Extract of Mango Kernel (Mangifera indica L.) |
title | Clinical Relevance and Antimicrobial Profiling of Methicillin-Resistant Staphylococcus aureus (MRSA) on Routine Antibiotics and Ethanol Extract of Mango Kernel (Mangifera indica L.) |
title_full | Clinical Relevance and Antimicrobial Profiling of Methicillin-Resistant Staphylococcus aureus (MRSA) on Routine Antibiotics and Ethanol Extract of Mango Kernel (Mangifera indica L.) |
title_fullStr | Clinical Relevance and Antimicrobial Profiling of Methicillin-Resistant Staphylococcus aureus (MRSA) on Routine Antibiotics and Ethanol Extract of Mango Kernel (Mangifera indica L.) |
title_full_unstemmed | Clinical Relevance and Antimicrobial Profiling of Methicillin-Resistant Staphylococcus aureus (MRSA) on Routine Antibiotics and Ethanol Extract of Mango Kernel (Mangifera indica L.) |
title_short | Clinical Relevance and Antimicrobial Profiling of Methicillin-Resistant Staphylococcus aureus (MRSA) on Routine Antibiotics and Ethanol Extract of Mango Kernel (Mangifera indica L.) |
title_sort | clinical relevance and antimicrobial profiling of methicillin-resistant staphylococcus aureus (mrsa) on routine antibiotics and ethanol extract of mango kernel (mangifera indica l.) |
topic | Research Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7049837/ https://www.ncbi.nlm.nih.gov/pubmed/32149104 http://dx.doi.org/10.1155/2020/4150678 |
work_keys_str_mv | AT albshabsheali clinicalrelevanceandantimicrobialprofilingofmethicillinresistantstaphylococcusaureusmrsaonroutineantibioticsandethanolextractofmangokernelmangiferaindical AT josephmartinrp clinicalrelevanceandantimicrobialprofilingofmethicillinresistantstaphylococcusaureusmrsaonroutineantibioticsandethanolextractofmangokernelmangiferaindical AT awadelgiedamgada clinicalrelevanceandantimicrobialprofilingofmethicillinresistantstaphylococcusaureusmrsaonroutineantibioticsandethanolextractofmangokernelmangiferaindical AT fadulabdallan clinicalrelevanceandantimicrobialprofilingofmethicillinresistantstaphylococcusaureusmrsaonroutineantibioticsandethanolextractofmangokernelmangiferaindical AT chandramoorthyharishc clinicalrelevanceandantimicrobialprofilingofmethicillinresistantstaphylococcusaureusmrsaonroutineantibioticsandethanolextractofmangokernelmangiferaindical AT hamidmohamede clinicalrelevanceandantimicrobialprofilingofmethicillinresistantstaphylococcusaureusmrsaonroutineantibioticsandethanolextractofmangokernelmangiferaindical |