Cargando…

Clinical Relevance and Antimicrobial Profiling of Methicillin-Resistant Staphylococcus aureus (MRSA) on Routine Antibiotics and Ethanol Extract of Mango Kernel (Mangifera indica L.)

Methicillin-resistant Staphylococcus aureus (MRSA) is known for serious health problems. Testing new inexpensive natural products such as mango kernel (Mangifera indica L., Anacardiaceae) may provide alternative and economically viable anti-MRSA drugs. In the current study, we screened clinical isol...

Descripción completa

Detalles Bibliográficos
Autores principales: Al Bshabshe, Ali, Joseph, Martin R. P., Awad El-Gied, Amgad A., Fadul, Abdalla N., Chandramoorthy, Harish C., Hamid, Mohamed E.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Hindawi 2020
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7049837/
https://www.ncbi.nlm.nih.gov/pubmed/32149104
http://dx.doi.org/10.1155/2020/4150678
_version_ 1783502525930930176
author Al Bshabshe, Ali
Joseph, Martin R. P.
Awad El-Gied, Amgad A.
Fadul, Abdalla N.
Chandramoorthy, Harish C.
Hamid, Mohamed E.
author_facet Al Bshabshe, Ali
Joseph, Martin R. P.
Awad El-Gied, Amgad A.
Fadul, Abdalla N.
Chandramoorthy, Harish C.
Hamid, Mohamed E.
author_sort Al Bshabshe, Ali
collection PubMed
description Methicillin-resistant Staphylococcus aureus (MRSA) is known for serious health problems. Testing new inexpensive natural products such as mango kernel (Mangifera indica L., Anacardiaceae) may provide alternative and economically viable anti-MRSA drugs. In the current study, we screened clinical isolates from Aseer Central Hospital, Saudi Arabia, during 2012–2017 for MRSA and tested an ethanolic extract of mango kernel for anti-MRSA activity. Brief confirmation of MRSA was performed by the Vitek 2 system, while antibiotic sensitivity of strains was tested for their clinical relevance. The In vitro disc diffusion method was used to test the anti-MRSA activity of the ethanolic mango kernel extract. The antimicrobial activity of mango kernel was compared to that of standard drugs (oxacillin and vancomycin). Of the identified 132 S. aureus strains, 42 (31.8%) were found to be MRSA and their prevalence showed a clear increase during the last two years (2016-2017; p < 0.001). MRSA strains showed 100% sensitivity to vancomycin, teicoplanin, linezolid, tetracycline, daptomycin, tigecycline, and tobramycin and 100% resistance to ampicillin and 98% to penicillin. The ethanolic extracts of mango kernel were found active against both S. aureus and the MRSA strains. Inhibitory activities (mean ± SE) were achieved at concentrations of 50 mg/mL (20.77 ± 0.61), 5 mg/mL (16.18 ± 0.34), and 0.5 mg/mL (8.39 ± 0.33) exceeding that of vancomycin (p=0.0162). MRSA strains were sensitive to mango kernel extracts when compared to vancomycin. Therefore, ethanolic extracts of mango kernel can be escalated to animal model studies as a promising leading anti-MRSA drug candidate and can be an economic alternative to high-priced synthetic antibiotics.
format Online
Article
Text
id pubmed-7049837
institution National Center for Biotechnology Information
language English
publishDate 2020
publisher Hindawi
record_format MEDLINE/PubMed
spelling pubmed-70498372020-03-08 Clinical Relevance and Antimicrobial Profiling of Methicillin-Resistant Staphylococcus aureus (MRSA) on Routine Antibiotics and Ethanol Extract of Mango Kernel (Mangifera indica L.) Al Bshabshe, Ali Joseph, Martin R. P. Awad El-Gied, Amgad A. Fadul, Abdalla N. Chandramoorthy, Harish C. Hamid, Mohamed E. Biomed Res Int Research Article Methicillin-resistant Staphylococcus aureus (MRSA) is known for serious health problems. Testing new inexpensive natural products such as mango kernel (Mangifera indica L., Anacardiaceae) may provide alternative and economically viable anti-MRSA drugs. In the current study, we screened clinical isolates from Aseer Central Hospital, Saudi Arabia, during 2012–2017 for MRSA and tested an ethanolic extract of mango kernel for anti-MRSA activity. Brief confirmation of MRSA was performed by the Vitek 2 system, while antibiotic sensitivity of strains was tested for their clinical relevance. The In vitro disc diffusion method was used to test the anti-MRSA activity of the ethanolic mango kernel extract. The antimicrobial activity of mango kernel was compared to that of standard drugs (oxacillin and vancomycin). Of the identified 132 S. aureus strains, 42 (31.8%) were found to be MRSA and their prevalence showed a clear increase during the last two years (2016-2017; p < 0.001). MRSA strains showed 100% sensitivity to vancomycin, teicoplanin, linezolid, tetracycline, daptomycin, tigecycline, and tobramycin and 100% resistance to ampicillin and 98% to penicillin. The ethanolic extracts of mango kernel were found active against both S. aureus and the MRSA strains. Inhibitory activities (mean ± SE) were achieved at concentrations of 50 mg/mL (20.77 ± 0.61), 5 mg/mL (16.18 ± 0.34), and 0.5 mg/mL (8.39 ± 0.33) exceeding that of vancomycin (p=0.0162). MRSA strains were sensitive to mango kernel extracts when compared to vancomycin. Therefore, ethanolic extracts of mango kernel can be escalated to animal model studies as a promising leading anti-MRSA drug candidate and can be an economic alternative to high-priced synthetic antibiotics. Hindawi 2020-02-18 /pmc/articles/PMC7049837/ /pubmed/32149104 http://dx.doi.org/10.1155/2020/4150678 Text en Copyright © 2020 Ali Al Bshabshe et al. http://creativecommons.org/licenses/by/4.0/ This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Research Article
Al Bshabshe, Ali
Joseph, Martin R. P.
Awad El-Gied, Amgad A.
Fadul, Abdalla N.
Chandramoorthy, Harish C.
Hamid, Mohamed E.
Clinical Relevance and Antimicrobial Profiling of Methicillin-Resistant Staphylococcus aureus (MRSA) on Routine Antibiotics and Ethanol Extract of Mango Kernel (Mangifera indica L.)
title Clinical Relevance and Antimicrobial Profiling of Methicillin-Resistant Staphylococcus aureus (MRSA) on Routine Antibiotics and Ethanol Extract of Mango Kernel (Mangifera indica L.)
title_full Clinical Relevance and Antimicrobial Profiling of Methicillin-Resistant Staphylococcus aureus (MRSA) on Routine Antibiotics and Ethanol Extract of Mango Kernel (Mangifera indica L.)
title_fullStr Clinical Relevance and Antimicrobial Profiling of Methicillin-Resistant Staphylococcus aureus (MRSA) on Routine Antibiotics and Ethanol Extract of Mango Kernel (Mangifera indica L.)
title_full_unstemmed Clinical Relevance and Antimicrobial Profiling of Methicillin-Resistant Staphylococcus aureus (MRSA) on Routine Antibiotics and Ethanol Extract of Mango Kernel (Mangifera indica L.)
title_short Clinical Relevance and Antimicrobial Profiling of Methicillin-Resistant Staphylococcus aureus (MRSA) on Routine Antibiotics and Ethanol Extract of Mango Kernel (Mangifera indica L.)
title_sort clinical relevance and antimicrobial profiling of methicillin-resistant staphylococcus aureus (mrsa) on routine antibiotics and ethanol extract of mango kernel (mangifera indica l.)
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7049837/
https://www.ncbi.nlm.nih.gov/pubmed/32149104
http://dx.doi.org/10.1155/2020/4150678
work_keys_str_mv AT albshabsheali clinicalrelevanceandantimicrobialprofilingofmethicillinresistantstaphylococcusaureusmrsaonroutineantibioticsandethanolextractofmangokernelmangiferaindical
AT josephmartinrp clinicalrelevanceandantimicrobialprofilingofmethicillinresistantstaphylococcusaureusmrsaonroutineantibioticsandethanolextractofmangokernelmangiferaindical
AT awadelgiedamgada clinicalrelevanceandantimicrobialprofilingofmethicillinresistantstaphylococcusaureusmrsaonroutineantibioticsandethanolextractofmangokernelmangiferaindical
AT fadulabdallan clinicalrelevanceandantimicrobialprofilingofmethicillinresistantstaphylococcusaureusmrsaonroutineantibioticsandethanolextractofmangokernelmangiferaindical
AT chandramoorthyharishc clinicalrelevanceandantimicrobialprofilingofmethicillinresistantstaphylococcusaureusmrsaonroutineantibioticsandethanolextractofmangokernelmangiferaindical
AT hamidmohamede clinicalrelevanceandantimicrobialprofilingofmethicillinresistantstaphylococcusaureusmrsaonroutineantibioticsandethanolextractofmangokernelmangiferaindical