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Comparative Genomics of Actinobacillus pleuropneumoniae Serotype 8 Reveals the Importance of Prophages in the Genetic Variability of the Species

Actinobacillus pleuropneumoniae is the etiologic agent of porcine pleuropneumonia. Currently, there are 18 different serotypes; the serotype 8 is the most widely distributed in the United States, Canada, United Kingdom, and southeastern Brazil. In this study, genomes of seven A. pleuropneumoniae ser...

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Autores principales: Prado, Isabelle Gonçalves de Oliveira, da Silva, Giarlã Cunha, Crispim, Josicelli Souza, Vidigal, Pedro Marcus Pereira, Nascimento, Moysés, Santana, Mateus Ferreira, Bazzolli, Denise Mara Soares
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Hindawi 2020
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7049842/
https://www.ncbi.nlm.nih.gov/pubmed/32149072
http://dx.doi.org/10.1155/2020/9354204
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author Prado, Isabelle Gonçalves de Oliveira
da Silva, Giarlã Cunha
Crispim, Josicelli Souza
Vidigal, Pedro Marcus Pereira
Nascimento, Moysés
Santana, Mateus Ferreira
Bazzolli, Denise Mara Soares
author_facet Prado, Isabelle Gonçalves de Oliveira
da Silva, Giarlã Cunha
Crispim, Josicelli Souza
Vidigal, Pedro Marcus Pereira
Nascimento, Moysés
Santana, Mateus Ferreira
Bazzolli, Denise Mara Soares
author_sort Prado, Isabelle Gonçalves de Oliveira
collection PubMed
description Actinobacillus pleuropneumoniae is the etiologic agent of porcine pleuropneumonia. Currently, there are 18 different serotypes; the serotype 8 is the most widely distributed in the United States, Canada, United Kingdom, and southeastern Brazil. In this study, genomes of seven A. pleuropneumoniae serotype 8 clinical isolates were compared to the other genomes of twelve serotypes. The analyses of serotype 8 genomes resulted in a set of 2352 protein-coding sequences. Of these sequences, 76.6% are present in all serotypes, 18.5% are shared with some serotypes, and 4.9% were differential. This differential portion was characterized as a series of hypothetical and regulatory protein sequences: mobile element sequence. Synteny analysis demonstrated possible events of gene recombination and acquisition by horizontal gene transfer (HGT) in this species. A total of 30 sequences related to prophages were identified in the genomes. These sequences represented 0.3 to 3.5% of the genome of the strains analyzed, and 16 of them contained complete prophages. Similarity analysis between complete prophage sequences evidenced a possible HGT with species belonging to the family Pasteurellaceae. Thus, mobile genetic elements, such as prophages, are important components of the differential portion of the A. pleuropneumoniae genome and demonstrate a central role in the evolution of the species. This study represents the first study done to understand the genome of A. pleuropneumoniae serotype 8.
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spelling pubmed-70498422020-03-07 Comparative Genomics of Actinobacillus pleuropneumoniae Serotype 8 Reveals the Importance of Prophages in the Genetic Variability of the Species Prado, Isabelle Gonçalves de Oliveira da Silva, Giarlã Cunha Crispim, Josicelli Souza Vidigal, Pedro Marcus Pereira Nascimento, Moysés Santana, Mateus Ferreira Bazzolli, Denise Mara Soares Int J Genomics Research Article Actinobacillus pleuropneumoniae is the etiologic agent of porcine pleuropneumonia. Currently, there are 18 different serotypes; the serotype 8 is the most widely distributed in the United States, Canada, United Kingdom, and southeastern Brazil. In this study, genomes of seven A. pleuropneumoniae serotype 8 clinical isolates were compared to the other genomes of twelve serotypes. The analyses of serotype 8 genomes resulted in a set of 2352 protein-coding sequences. Of these sequences, 76.6% are present in all serotypes, 18.5% are shared with some serotypes, and 4.9% were differential. This differential portion was characterized as a series of hypothetical and regulatory protein sequences: mobile element sequence. Synteny analysis demonstrated possible events of gene recombination and acquisition by horizontal gene transfer (HGT) in this species. A total of 30 sequences related to prophages were identified in the genomes. These sequences represented 0.3 to 3.5% of the genome of the strains analyzed, and 16 of them contained complete prophages. Similarity analysis between complete prophage sequences evidenced a possible HGT with species belonging to the family Pasteurellaceae. Thus, mobile genetic elements, such as prophages, are important components of the differential portion of the A. pleuropneumoniae genome and demonstrate a central role in the evolution of the species. This study represents the first study done to understand the genome of A. pleuropneumoniae serotype 8. Hindawi 2020-02-18 /pmc/articles/PMC7049842/ /pubmed/32149072 http://dx.doi.org/10.1155/2020/9354204 Text en Copyright © 2020 Isabelle Gonçalves de Oliveira Prado et al. http://creativecommons.org/licenses/by/4.0/ This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Research Article
Prado, Isabelle Gonçalves de Oliveira
da Silva, Giarlã Cunha
Crispim, Josicelli Souza
Vidigal, Pedro Marcus Pereira
Nascimento, Moysés
Santana, Mateus Ferreira
Bazzolli, Denise Mara Soares
Comparative Genomics of Actinobacillus pleuropneumoniae Serotype 8 Reveals the Importance of Prophages in the Genetic Variability of the Species
title Comparative Genomics of Actinobacillus pleuropneumoniae Serotype 8 Reveals the Importance of Prophages in the Genetic Variability of the Species
title_full Comparative Genomics of Actinobacillus pleuropneumoniae Serotype 8 Reveals the Importance of Prophages in the Genetic Variability of the Species
title_fullStr Comparative Genomics of Actinobacillus pleuropneumoniae Serotype 8 Reveals the Importance of Prophages in the Genetic Variability of the Species
title_full_unstemmed Comparative Genomics of Actinobacillus pleuropneumoniae Serotype 8 Reveals the Importance of Prophages in the Genetic Variability of the Species
title_short Comparative Genomics of Actinobacillus pleuropneumoniae Serotype 8 Reveals the Importance of Prophages in the Genetic Variability of the Species
title_sort comparative genomics of actinobacillus pleuropneumoniae serotype 8 reveals the importance of prophages in the genetic variability of the species
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7049842/
https://www.ncbi.nlm.nih.gov/pubmed/32149072
http://dx.doi.org/10.1155/2020/9354204
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